Cyclolepis genistoides aqueous extract as source of neuroprotective agents

Autores
Alza, Natalia Paola; Cavallaro, Valeria; Benzi Juncos, Oriana Nicole; Murray, Ana Paula; Salvador, Gabriela Alejandra
Año de publicación
2021
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Since oxidative stress (OS) is a main component in neurodegenerative diseases, targeting this causative agent constitutes an important approach in drug discovery. In previous studies, we have selected the aqueous extract of Cyclolepis genistoides from a set of medicinal plants based on its ability to diminish metal-induced OS cellular markers. The aim of this work was to characterize the mechanism by which C. genistoides extract protected cells against OS. For this purpose, human neuroblastoma cells (IMR-32) were exposed to C. genistoides extract under conditions favoring OS (ferric ammonium citrate -FAC- exposure). C. genistoides extract (20 μg/mL) diminished the generation of superoxide anion in the presence of FAC. Moreover, the extract increased catalase activity when cells were exposed to FAC. Additionally, C. genistoides extract triggered the nuclear translocation of Nrf2, a cytoprotective transcription factor involved in antioxidant enzyme expression. The determination of flavonoids in C. genistoides extract revealed 16.1 ± 0.1 mg quercetin equivalents/g. To identify the bioactive components, 9 fractions (A-I) were obtained after a bio-guided fractionation. The evaluation of the main fractions F, G and H (20 μg/mL) showed that fractions F and H exposure reduced reactive oxygen species (ROS) production induced by FAC; however, fraction G treatment increased ROS levels. Until now, a sesquiterpene lactone was identified in fraction F. Our findings suggest that C. genistoides extract exerts the protective effect via the activation of cellular antioxidant defenses. Further studies are necessary to identify which compounds are responsible for the neuroprotective effect through Nrf2 modulation
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cavallaro, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
7th International Electronic Conference on Medicinal Chemistry
Basel
Bélgica
Sciforum
Materia
Cyclolepis genistoides
cellular oxidative stress
neuroprotective agents
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/175767
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Cyclolepis genistoides aqueous extract as source of neuroprotective agentsAlza, Natalia PaolaCavallaro, ValeriaBenzi Juncos, Oriana NicoleMurray, Ana PaulaSalvador, Gabriela AlejandraCyclolepis genistoidescellular oxidative stressneuroprotective agentshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Since oxidative stress (OS) is a main component in neurodegenerative diseases, targeting this causative agent constitutes an important approach in drug discovery. In previous studies, we have selected the aqueous extract of Cyclolepis genistoides from a set of medicinal plants based on its ability to diminish metal-induced OS cellular markers. The aim of this work was to characterize the mechanism by which C. genistoides extract protected cells against OS. For this purpose, human neuroblastoma cells (IMR-32) were exposed to C. genistoides extract under conditions favoring OS (ferric ammonium citrate -FAC- exposure). C. genistoides extract (20 μg/mL) diminished the generation of superoxide anion in the presence of FAC. Moreover, the extract increased catalase activity when cells were exposed to FAC. Additionally, C. genistoides extract triggered the nuclear translocation of Nrf2, a cytoprotective transcription factor involved in antioxidant enzyme expression. The determination of flavonoids in C. genistoides extract revealed 16.1 ± 0.1 mg quercetin equivalents/g. To identify the bioactive components, 9 fractions (A-I) were obtained after a bio-guided fractionation. The evaluation of the main fractions F, G and H (20 μg/mL) showed that fractions F and H exposure reduced reactive oxygen species (ROS) production induced by FAC; however, fraction G treatment increased ROS levels. Until now, a sesquiterpene lactone was identified in fraction F. Our findings suggest that C. genistoides extract exerts the protective effect via the activation of cellular antioxidant defenses. Further studies are necessary to identify which compounds are responsible for the neuroprotective effect through Nrf2 modulationFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Cavallaro, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina7th International Electronic Conference on Medicinal ChemistryBaselBélgicaSciforumMultidisciplinary Digital Publishing Institute2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciatext/plainapplication/pdfhttp://hdl.handle.net/11336/175767Cyclolepis genistoides aqueous extract as source of neuroprotective agents; 7th International Electronic Conference on Medicinal Chemistry; Basel; Bélgica; 2021; 1-9CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://ecmc2021.sciforum.netinfo:eu-repo/semantics/altIdentifier/url/https://sciforum.net/paper/view/11536info:eu-repo/semantics/altIdentifier/doi/10.3390/ECMC2021-11536Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:36:23Zoai:ri.conicet.gov.ar:11336/175767instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:36:23.616CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cyclolepis genistoides aqueous extract as source of neuroprotective agents
title Cyclolepis genistoides aqueous extract as source of neuroprotective agents
spellingShingle Cyclolepis genistoides aqueous extract as source of neuroprotective agents
Alza, Natalia Paola
Cyclolepis genistoides
cellular oxidative stress
neuroprotective agents
title_short Cyclolepis genistoides aqueous extract as source of neuroprotective agents
title_full Cyclolepis genistoides aqueous extract as source of neuroprotective agents
title_fullStr Cyclolepis genistoides aqueous extract as source of neuroprotective agents
title_full_unstemmed Cyclolepis genistoides aqueous extract as source of neuroprotective agents
title_sort Cyclolepis genistoides aqueous extract as source of neuroprotective agents
dc.creator.none.fl_str_mv Alza, Natalia Paola
Cavallaro, Valeria
Benzi Juncos, Oriana Nicole
Murray, Ana Paula
Salvador, Gabriela Alejandra
author Alza, Natalia Paola
author_facet Alza, Natalia Paola
Cavallaro, Valeria
Benzi Juncos, Oriana Nicole
Murray, Ana Paula
Salvador, Gabriela Alejandra
author_role author
author2 Cavallaro, Valeria
Benzi Juncos, Oriana Nicole
Murray, Ana Paula
Salvador, Gabriela Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Cyclolepis genistoides
cellular oxidative stress
neuroprotective agents
topic Cyclolepis genistoides
cellular oxidative stress
neuroprotective agents
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Since oxidative stress (OS) is a main component in neurodegenerative diseases, targeting this causative agent constitutes an important approach in drug discovery. In previous studies, we have selected the aqueous extract of Cyclolepis genistoides from a set of medicinal plants based on its ability to diminish metal-induced OS cellular markers. The aim of this work was to characterize the mechanism by which C. genistoides extract protected cells against OS. For this purpose, human neuroblastoma cells (IMR-32) were exposed to C. genistoides extract under conditions favoring OS (ferric ammonium citrate -FAC- exposure). C. genistoides extract (20 μg/mL) diminished the generation of superoxide anion in the presence of FAC. Moreover, the extract increased catalase activity when cells were exposed to FAC. Additionally, C. genistoides extract triggered the nuclear translocation of Nrf2, a cytoprotective transcription factor involved in antioxidant enzyme expression. The determination of flavonoids in C. genistoides extract revealed 16.1 ± 0.1 mg quercetin equivalents/g. To identify the bioactive components, 9 fractions (A-I) were obtained after a bio-guided fractionation. The evaluation of the main fractions F, G and H (20 μg/mL) showed that fractions F and H exposure reduced reactive oxygen species (ROS) production induced by FAC; however, fraction G treatment increased ROS levels. Until now, a sesquiterpene lactone was identified in fraction F. Our findings suggest that C. genistoides extract exerts the protective effect via the activation of cellular antioxidant defenses. Further studies are necessary to identify which compounds are responsible for the neuroprotective effect through Nrf2 modulation
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Cavallaro, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
7th International Electronic Conference on Medicinal Chemistry
Basel
Bélgica
Sciforum
description Since oxidative stress (OS) is a main component in neurodegenerative diseases, targeting this causative agent constitutes an important approach in drug discovery. In previous studies, we have selected the aqueous extract of Cyclolepis genistoides from a set of medicinal plants based on its ability to diminish metal-induced OS cellular markers. The aim of this work was to characterize the mechanism by which C. genistoides extract protected cells against OS. For this purpose, human neuroblastoma cells (IMR-32) were exposed to C. genistoides extract under conditions favoring OS (ferric ammonium citrate -FAC- exposure). C. genistoides extract (20 μg/mL) diminished the generation of superoxide anion in the presence of FAC. Moreover, the extract increased catalase activity when cells were exposed to FAC. Additionally, C. genistoides extract triggered the nuclear translocation of Nrf2, a cytoprotective transcription factor involved in antioxidant enzyme expression. The determination of flavonoids in C. genistoides extract revealed 16.1 ± 0.1 mg quercetin equivalents/g. To identify the bioactive components, 9 fractions (A-I) were obtained after a bio-guided fractionation. The evaluation of the main fractions F, G and H (20 μg/mL) showed that fractions F and H exposure reduced reactive oxygen species (ROS) production induced by FAC; however, fraction G treatment increased ROS levels. Until now, a sesquiterpene lactone was identified in fraction F. Our findings suggest that C. genistoides extract exerts the protective effect via the activation of cellular antioxidant defenses. Further studies are necessary to identify which compounds are responsible for the neuroprotective effect through Nrf2 modulation
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/175767
Cyclolepis genistoides aqueous extract as source of neuroprotective agents; 7th International Electronic Conference on Medicinal Chemistry; Basel; Bélgica; 2021; 1-9
CONICET Digital
CONICET
url http://hdl.handle.net/11336/175767
identifier_str_mv Cyclolepis genistoides aqueous extract as source of neuroprotective agents; 7th International Electronic Conference on Medicinal Chemistry; Basel; Bélgica; 2021; 1-9
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
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info:eu-repo/semantics/altIdentifier/url/https://sciforum.net/paper/view/11536
info:eu-repo/semantics/altIdentifier/doi/10.3390/ECMC2021-11536
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv text/plain
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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