Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells

Autores
Alza, Natalia Paola; Benzi Juncos, Oriana Nicole; Cavallaro, Valeria; Pferschy-Wenzig, Eva-Maria; Pirker, Teresa; Bauer, Rudolf; Murray, Ana Paula; Salvador, Gabriela Alejandra
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ethnopharmacological relevance: Cyclolepis genistoides has been traditionally used in Argentina, Chile and Paraguay in chronic diseases to intervene pain and inflammation-related conditions, suggesting a strong anti-inflammatory potential, which may also be useful to target neurodegenerative diseases (ND). However, the anti-(neuro)in- flammatory and antioxidant effects of C. genistoides has not been assessed in neurodegeneration models. Aim of the study: To evaluate the antioxidant and anti-inflammatory potential of C. genistoides aqueous extract and its main constituent deacylcynaropicrin (DAC) using neuronal and glial cultures under iron-induced oxidative stress, a common condition in ND. Methods: The metabolite profile of C. genistoides extract was analyzed by UHPLC-DAD-HRMS, and DAC was isolated. The effects of C. genistoides and DAC on iron-induced oxidative stress were assessed using H2DCFDA, NBT, TBARS, and BODIPY581/591 C11 assays, along with NRF2 status. DAC anti-inflammatory activity was evaluated by NO production, and the expression of NFκB, COX-2, IL-1β and GFAP in glial cells. Results: Pretreatment with C. genistoides extract (20 μg/mL) reduced reactive oxygen species and lipid peroxi- dation in iron-treated IMR-32 cells. The extract promoted NRF2 nuclear translocation and upregulated GCLc and catalase. DAC exhibited similar antioxidant effects. Furthermore, DAC (10 μM) demonstrated anti-inflammatory activity by inhibiting NO production, NFκB nuclear localization, and COX-2 and IL-1β expression in macrophages and glial cells, and it reduced reactivity and lipid peroxidation in astrocytes. Conclusions: Our work shows for the first time that C. genistoides and DAC display pronounced antioxidant and anti-inflammatory effects in cellular ND models, corroborating the scientific basis of the traditional use
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Cavallaro, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pferschy-Wenzig, Eva-Maria. University of Graz; Austria
Fil: Pirker, Teresa. University of Graz; Austria
Fil: Bauer, Rudolf. University of Graz; Austria
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Materia
Deacylcynaropicrin
Cyclolepis genistoides
Iron-induced oxidative stress
Anti-Neuroinflammation
Neurodegenerative diseases
Nivel de accesibilidad
acceso embargado
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271558

id CONICETDig_59ecfb75a59754917839399f50771b3a
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network_name_str CONICET Digital (CONICET)
spelling Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cellsAlza, Natalia PaolaBenzi Juncos, Oriana NicoleCavallaro, ValeriaPferschy-Wenzig, Eva-MariaPirker, TeresaBauer, RudolfMurray, Ana PaulaSalvador, Gabriela AlejandraDeacylcynaropicrinCyclolepis genistoidesIron-induced oxidative stressAnti-NeuroinflammationNeurodegenerative diseaseshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Ethnopharmacological relevance: Cyclolepis genistoides has been traditionally used in Argentina, Chile and Paraguay in chronic diseases to intervene pain and inflammation-related conditions, suggesting a strong anti-inflammatory potential, which may also be useful to target neurodegenerative diseases (ND). However, the anti-(neuro)in- flammatory and antioxidant effects of C. genistoides has not been assessed in neurodegeneration models. Aim of the study: To evaluate the antioxidant and anti-inflammatory potential of C. genistoides aqueous extract and its main constituent deacylcynaropicrin (DAC) using neuronal and glial cultures under iron-induced oxidative stress, a common condition in ND. Methods: The metabolite profile of C. genistoides extract was analyzed by UHPLC-DAD-HRMS, and DAC was isolated. The effects of C. genistoides and DAC on iron-induced oxidative stress were assessed using H2DCFDA, NBT, TBARS, and BODIPY581/591 C11 assays, along with NRF2 status. DAC anti-inflammatory activity was evaluated by NO production, and the expression of NFκB, COX-2, IL-1β and GFAP in glial cells. Results: Pretreatment with C. genistoides extract (20 μg/mL) reduced reactive oxygen species and lipid peroxi- dation in iron-treated IMR-32 cells. The extract promoted NRF2 nuclear translocation and upregulated GCLc and catalase. DAC exhibited similar antioxidant effects. Furthermore, DAC (10 μM) demonstrated anti-inflammatory activity by inhibiting NO production, NFκB nuclear localization, and COX-2 and IL-1β expression in macrophages and glial cells, and it reduced reactivity and lipid peroxidation in astrocytes. Conclusions: Our work shows for the first time that C. genistoides and DAC display pronounced antioxidant and anti-inflammatory effects in cellular ND models, corroborating the scientific basis of the traditional useFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Cavallaro, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Pferschy-Wenzig, Eva-Maria. University of Graz; AustriaFil: Pirker, Teresa. University of Graz; AustriaFil: Bauer, Rudolf. University of Graz; AustriaFil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaElsevier2025-08-05info:eu-repo/date/embargoEnd/2026-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271558Alza, Natalia Paola; Benzi Juncos, Oriana Nicole; Cavallaro, Valeria; Pferschy-Wenzig, Eva-Maria; Pirker, Teresa; et al.; Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells; Elsevier ; Journal of Ethnopharmacology; 353; A; 5-8-2025; 1-140378-87411872-7573CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0378874125010402info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2025.120348info:eu-repo/semantics/embargoedAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:48:00Zoai:ri.conicet.gov.ar:11336/271558instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:48:01.268CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
title Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
spellingShingle Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
Alza, Natalia Paola
Deacylcynaropicrin
Cyclolepis genistoides
Iron-induced oxidative stress
Anti-Neuroinflammation
Neurodegenerative diseases
title_short Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
title_full Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
title_fullStr Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
title_full_unstemmed Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
title_sort Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells
dc.creator.none.fl_str_mv Alza, Natalia Paola
Benzi Juncos, Oriana Nicole
Cavallaro, Valeria
Pferschy-Wenzig, Eva-Maria
Pirker, Teresa
Bauer, Rudolf
Murray, Ana Paula
Salvador, Gabriela Alejandra
author Alza, Natalia Paola
author_facet Alza, Natalia Paola
Benzi Juncos, Oriana Nicole
Cavallaro, Valeria
Pferschy-Wenzig, Eva-Maria
Pirker, Teresa
Bauer, Rudolf
Murray, Ana Paula
Salvador, Gabriela Alejandra
author_role author
author2 Benzi Juncos, Oriana Nicole
Cavallaro, Valeria
Pferschy-Wenzig, Eva-Maria
Pirker, Teresa
Bauer, Rudolf
Murray, Ana Paula
Salvador, Gabriela Alejandra
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Deacylcynaropicrin
Cyclolepis genistoides
Iron-induced oxidative stress
Anti-Neuroinflammation
Neurodegenerative diseases
topic Deacylcynaropicrin
Cyclolepis genistoides
Iron-induced oxidative stress
Anti-Neuroinflammation
Neurodegenerative diseases
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ethnopharmacological relevance: Cyclolepis genistoides has been traditionally used in Argentina, Chile and Paraguay in chronic diseases to intervene pain and inflammation-related conditions, suggesting a strong anti-inflammatory potential, which may also be useful to target neurodegenerative diseases (ND). However, the anti-(neuro)in- flammatory and antioxidant effects of C. genistoides has not been assessed in neurodegeneration models. Aim of the study: To evaluate the antioxidant and anti-inflammatory potential of C. genistoides aqueous extract and its main constituent deacylcynaropicrin (DAC) using neuronal and glial cultures under iron-induced oxidative stress, a common condition in ND. Methods: The metabolite profile of C. genistoides extract was analyzed by UHPLC-DAD-HRMS, and DAC was isolated. The effects of C. genistoides and DAC on iron-induced oxidative stress were assessed using H2DCFDA, NBT, TBARS, and BODIPY581/591 C11 assays, along with NRF2 status. DAC anti-inflammatory activity was evaluated by NO production, and the expression of NFκB, COX-2, IL-1β and GFAP in glial cells. Results: Pretreatment with C. genistoides extract (20 μg/mL) reduced reactive oxygen species and lipid peroxi- dation in iron-treated IMR-32 cells. The extract promoted NRF2 nuclear translocation and upregulated GCLc and catalase. DAC exhibited similar antioxidant effects. Furthermore, DAC (10 μM) demonstrated anti-inflammatory activity by inhibiting NO production, NFκB nuclear localization, and COX-2 and IL-1β expression in macrophages and glial cells, and it reduced reactivity and lipid peroxidation in astrocytes. Conclusions: Our work shows for the first time that C. genistoides and DAC display pronounced antioxidant and anti-inflammatory effects in cellular ND models, corroborating the scientific basis of the traditional use
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Cavallaro, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pferschy-Wenzig, Eva-Maria. University of Graz; Austria
Fil: Pirker, Teresa. University of Graz; Austria
Fil: Bauer, Rudolf. University of Graz; Austria
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description Ethnopharmacological relevance: Cyclolepis genistoides has been traditionally used in Argentina, Chile and Paraguay in chronic diseases to intervene pain and inflammation-related conditions, suggesting a strong anti-inflammatory potential, which may also be useful to target neurodegenerative diseases (ND). However, the anti-(neuro)in- flammatory and antioxidant effects of C. genistoides has not been assessed in neurodegeneration models. Aim of the study: To evaluate the antioxidant and anti-inflammatory potential of C. genistoides aqueous extract and its main constituent deacylcynaropicrin (DAC) using neuronal and glial cultures under iron-induced oxidative stress, a common condition in ND. Methods: The metabolite profile of C. genistoides extract was analyzed by UHPLC-DAD-HRMS, and DAC was isolated. The effects of C. genistoides and DAC on iron-induced oxidative stress were assessed using H2DCFDA, NBT, TBARS, and BODIPY581/591 C11 assays, along with NRF2 status. DAC anti-inflammatory activity was evaluated by NO production, and the expression of NFκB, COX-2, IL-1β and GFAP in glial cells. Results: Pretreatment with C. genistoides extract (20 μg/mL) reduced reactive oxygen species and lipid peroxi- dation in iron-treated IMR-32 cells. The extract promoted NRF2 nuclear translocation and upregulated GCLc and catalase. DAC exhibited similar antioxidant effects. Furthermore, DAC (10 μM) demonstrated anti-inflammatory activity by inhibiting NO production, NFκB nuclear localization, and COX-2 and IL-1β expression in macrophages and glial cells, and it reduced reactivity and lipid peroxidation in astrocytes. Conclusions: Our work shows for the first time that C. genistoides and DAC display pronounced antioxidant and anti-inflammatory effects in cellular ND models, corroborating the scientific basis of the traditional use
publishDate 2025
dc.date.none.fl_str_mv 2025-08-05
info:eu-repo/date/embargoEnd/2026-09-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271558
Alza, Natalia Paola; Benzi Juncos, Oriana Nicole; Cavallaro, Valeria; Pferschy-Wenzig, Eva-Maria; Pirker, Teresa; et al.; Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells; Elsevier ; Journal of Ethnopharmacology; 353; A; 5-8-2025; 1-14
0378-8741
1872-7573
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271558
identifier_str_mv Alza, Natalia Paola; Benzi Juncos, Oriana Nicole; Cavallaro, Valeria; Pferschy-Wenzig, Eva-Maria; Pirker, Teresa; et al.; Deacylcynaropicrin from Cyclolepis genistoides attenuates iron-induced oxidative stress and inflammatory signaling in neuron and glial cells; Elsevier ; Journal of Ethnopharmacology; 353; A; 5-8-2025; 1-14
0378-8741
1872-7573
CONICET Digital
CONICET
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2025.120348
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