Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress

Autores
Alza, Natalia Paola; Benzi Juncos, Oriana Nicole; Murray, Ana Paula; Salvador, Gabriela Alejandra
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The development of neuroprotective agents constitutes one of the most promising strategies to treat neurodegenerative disorders, such as Parkinson’s disease (PD). Oxidative stress (OS) is a major contributor to the death of dopaminergic neurons in PD. In the present work, we investigated the effect of aqueous extracts of three Argentinian medicinal plants, Agalinis genistifolia (Cham. & Schltdl.) D’Arcy, Cyclolepis genistoides Gillies ex D. Don and Margyricarpus pinnatus (Lam.) Kuntze, on cellular models of metal-induced OS. These species have been traditionally used to treat PD-related symptoms, such as paralysis for A. genistifolia and inflammation for C. genistoides and M. pinnatus. To evaluate the potential neuroprotective activity of the aqueous extracts, we used the human neuroblastoma cell line IMR-32 exposed to ferric ammonium citrate (FAC) as an OS inducer. Whereas cells exposed to FAC exhibited increased levels of reactive oxygen species (ROS) after the treatment with A. genistifolia and M. pinnatus (50 µg/mL extract), the exposure to C. genistoides extract at 20 µg/mL showed a reduction in ROS levels. In line with this finding, we found that C. genistoides treatment decreased lipid peroxidation under the same experimental conditions (20 µg/mL). Furthermore, the induction of ROS production by manganese in IMR-32 cells and by FAC in N27 rat dopaminergic cells was attenuated by the exposure to C. genistoides extract. Our results suggest that the aqueous extract of C. genistoides has potential as a source of neuroprotective agents that can target OS, a hallmark of neuronal death in PD
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
The 24th International Electronic Conference on Synthetic Organic Chemistry
España
Electronic Conference on Synthetic Organic Chemistry
Materia
NEUROPROTECTIVE AGENTS
OXIDATIVE STRESS
MEDICINAL PLANTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/201890
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stressAlza, Natalia PaolaBenzi Juncos, Oriana NicoleMurray, Ana PaulaSalvador, Gabriela AlejandraNEUROPROTECTIVE AGENTSOXIDATIVE STRESSMEDICINAL PLANTShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The development of neuroprotective agents constitutes one of the most promising strategies to treat neurodegenerative disorders, such as Parkinson’s disease (PD). Oxidative stress (OS) is a major contributor to the death of dopaminergic neurons in PD. In the present work, we investigated the effect of aqueous extracts of three Argentinian medicinal plants, Agalinis genistifolia (Cham. & Schltdl.) D’Arcy, Cyclolepis genistoides Gillies ex D. Don and Margyricarpus pinnatus (Lam.) Kuntze, on cellular models of metal-induced OS. These species have been traditionally used to treat PD-related symptoms, such as paralysis for A. genistifolia and inflammation for C. genistoides and M. pinnatus. To evaluate the potential neuroprotective activity of the aqueous extracts, we used the human neuroblastoma cell line IMR-32 exposed to ferric ammonium citrate (FAC) as an OS inducer. Whereas cells exposed to FAC exhibited increased levels of reactive oxygen species (ROS) after the treatment with A. genistifolia and M. pinnatus (50 µg/mL extract), the exposure to C. genistoides extract at 20 µg/mL showed a reduction in ROS levels. In line with this finding, we found that C. genistoides treatment decreased lipid peroxidation under the same experimental conditions (20 µg/mL). Furthermore, the induction of ROS production by manganese in IMR-32 cells and by FAC in N27 rat dopaminergic cells was attenuated by the exposure to C. genistoides extract. Our results suggest that the aqueous extract of C. genistoides has potential as a source of neuroprotective agents that can target OS, a hallmark of neuronal death in PDFil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; ArgentinaFil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaThe 24th International Electronic Conference on Synthetic Organic ChemistryEspañaElectronic Conference on Synthetic Organic ChemistryMultidisciplinary Digital Publishing InstituteSeijas Vázquez, Julio A.2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/201890Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress; The 24th International Electronic Conference on Synthetic Organic Chemistry ; España; 2020; 1-62504-3900CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ecsoc-24.sciforum.net/info:eu-repo/semantics/altIdentifier/doi/10.3390/ecsoc-24-08344Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:12:40Zoai:ri.conicet.gov.ar:11336/201890instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:12:40.68CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
title Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
spellingShingle Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
Alza, Natalia Paola
NEUROPROTECTIVE AGENTS
OXIDATIVE STRESS
MEDICINAL PLANTS
title_short Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
title_full Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
title_fullStr Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
title_full_unstemmed Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
title_sort Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress
dc.creator.none.fl_str_mv Alza, Natalia Paola
Benzi Juncos, Oriana Nicole
Murray, Ana Paula
Salvador, Gabriela Alejandra
author Alza, Natalia Paola
author_facet Alza, Natalia Paola
Benzi Juncos, Oriana Nicole
Murray, Ana Paula
Salvador, Gabriela Alejandra
author_role author
author2 Benzi Juncos, Oriana Nicole
Murray, Ana Paula
Salvador, Gabriela Alejandra
author2_role author
author
author
dc.contributor.none.fl_str_mv Seijas Vázquez, Julio A.
dc.subject.none.fl_str_mv NEUROPROTECTIVE AGENTS
OXIDATIVE STRESS
MEDICINAL PLANTS
topic NEUROPROTECTIVE AGENTS
OXIDATIVE STRESS
MEDICINAL PLANTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of neuroprotective agents constitutes one of the most promising strategies to treat neurodegenerative disorders, such as Parkinson’s disease (PD). Oxidative stress (OS) is a major contributor to the death of dopaminergic neurons in PD. In the present work, we investigated the effect of aqueous extracts of three Argentinian medicinal plants, Agalinis genistifolia (Cham. & Schltdl.) D’Arcy, Cyclolepis genistoides Gillies ex D. Don and Margyricarpus pinnatus (Lam.) Kuntze, on cellular models of metal-induced OS. These species have been traditionally used to treat PD-related symptoms, such as paralysis for A. genistifolia and inflammation for C. genistoides and M. pinnatus. To evaluate the potential neuroprotective activity of the aqueous extracts, we used the human neuroblastoma cell line IMR-32 exposed to ferric ammonium citrate (FAC) as an OS inducer. Whereas cells exposed to FAC exhibited increased levels of reactive oxygen species (ROS) after the treatment with A. genistifolia and M. pinnatus (50 µg/mL extract), the exposure to C. genistoides extract at 20 µg/mL showed a reduction in ROS levels. In line with this finding, we found that C. genistoides treatment decreased lipid peroxidation under the same experimental conditions (20 µg/mL). Furthermore, the induction of ROS production by manganese in IMR-32 cells and by FAC in N27 rat dopaminergic cells was attenuated by the exposure to C. genistoides extract. Our results suggest that the aqueous extract of C. genistoides has potential as a source of neuroprotective agents that can target OS, a hallmark of neuronal death in PD
Fil: Alza, Natalia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Benzi Juncos, Oriana Nicole. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Murray, Ana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Salvador, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
The 24th International Electronic Conference on Synthetic Organic Chemistry
España
Electronic Conference on Synthetic Organic Chemistry
description The development of neuroprotective agents constitutes one of the most promising strategies to treat neurodegenerative disorders, such as Parkinson’s disease (PD). Oxidative stress (OS) is a major contributor to the death of dopaminergic neurons in PD. In the present work, we investigated the effect of aqueous extracts of three Argentinian medicinal plants, Agalinis genistifolia (Cham. & Schltdl.) D’Arcy, Cyclolepis genistoides Gillies ex D. Don and Margyricarpus pinnatus (Lam.) Kuntze, on cellular models of metal-induced OS. These species have been traditionally used to treat PD-related symptoms, such as paralysis for A. genistifolia and inflammation for C. genistoides and M. pinnatus. To evaluate the potential neuroprotective activity of the aqueous extracts, we used the human neuroblastoma cell line IMR-32 exposed to ferric ammonium citrate (FAC) as an OS inducer. Whereas cells exposed to FAC exhibited increased levels of reactive oxygen species (ROS) after the treatment with A. genistifolia and M. pinnatus (50 µg/mL extract), the exposure to C. genistoides extract at 20 µg/mL showed a reduction in ROS levels. In line with this finding, we found that C. genistoides treatment decreased lipid peroxidation under the same experimental conditions (20 µg/mL). Furthermore, the induction of ROS production by manganese in IMR-32 cells and by FAC in N27 rat dopaminergic cells was attenuated by the exposure to C. genistoides extract. Our results suggest that the aqueous extract of C. genistoides has potential as a source of neuroprotective agents that can target OS, a hallmark of neuronal death in PD
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/201890
Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress; The 24th International Electronic Conference on Synthetic Organic Chemistry ; España; 2020; 1-6
2504-3900
CONICET Digital
CONICET
url http://hdl.handle.net/11336/201890
identifier_str_mv Protective effect of Cyclolepis genistoides aqueous extract against cellular oxidative stress; The 24th International Electronic Conference on Synthetic Organic Chemistry ; España; 2020; 1-6
2504-3900
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.3390/ecsoc-24-08344
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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