Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent

Autores
Elia Dazat, Ricardo Ariel; Mammana, Sabrina Belén; Canizo, Brenda Vanina; Silva, María Fernanda; Gomez, Federico Jose Vicente
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ergosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescent natural deep eutectic solvents (HPB-FLUODES) to determine ERG by fluorescence detection in edible mushrooms. The proposed approach involves a green sample preparation and a low cost instrumental technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis (2.4 samples per minute). The experimental variables, such as the type of HPB-FLUODES, sample/solvent mass/volume ratio, time, and temperature of extraction were studied and optimized. The selected variables were HPB-FLUODES thymol:lactic acid 1:2, 2.5 mg of sample, 1 mL of HPB-FLUODES, and 45 min of extraction at 40°C. The LOD and LOQ were 0.09 µg g−1 and 0.29 µg g−1, respectively. The results show an enhancement of ERG fluorescence signal of 1825% against the ERG signal in methanol media. The present approach shows outstanding green performance using AGREE®, AGREEprep® and WAC principles. Finally, the analysis of edible mushrooms demonstrates the feasibility of the proposed green methodology.
Fil: Elia Dazat, Ricardo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Mammana, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Canizo, Brenda Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Materia
ENHANCED FLUORESCENCE
ERGOSTEROL
FOOD
GREEN CHEMISTRY
HYDROPHOBIC FLUORESCENT NATURAL DEEP EUTECTIC SOLVENTS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/197933
Acceder
id CONICETDig_12c3d6560e6e96e7d35558857b52f2c2
oai_identifier_str oai:ri.conicet.gov.ar:11336/197933
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solventElia Dazat, Ricardo ArielMammana, Sabrina BelénCanizo, Brenda VaninaSilva, María FernandaGomez, Federico Jose VicenteENHANCED FLUORESCENCEERGOSTEROLFOODGREEN CHEMISTRYHYDROPHOBIC FLUORESCENT NATURAL DEEP EUTECTIC SOLVENTShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Ergosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescent natural deep eutectic solvents (HPB-FLUODES) to determine ERG by fluorescence detection in edible mushrooms. The proposed approach involves a green sample preparation and a low cost instrumental technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis (2.4 samples per minute). The experimental variables, such as the type of HPB-FLUODES, sample/solvent mass/volume ratio, time, and temperature of extraction were studied and optimized. The selected variables were HPB-FLUODES thymol:lactic acid 1:2, 2.5 mg of sample, 1 mL of HPB-FLUODES, and 45 min of extraction at 40°C. The LOD and LOQ were 0.09 µg g−1 and 0.29 µg g−1, respectively. The results show an enhancement of ERG fluorescence signal of 1825% against the ERG signal in methanol media. The present approach shows outstanding green performance using AGREE®, AGREEprep® and WAC principles. Finally, the analysis of edible mushrooms demonstrates the feasibility of the proposed green methodology.Fil: Elia Dazat, Ricardo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Mammana, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Canizo, Brenda Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; ArgentinaElsevier Science2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/197933Elia Dazat, Ricardo Ariel; Mammana, Sabrina Belén; Canizo, Brenda Vanina; Silva, María Fernanda; Gomez, Federico Jose Vicente; Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent; Elsevier Science; Green Analytical Chemistry; 3; 12-2022; 1-62772-5774CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2772577422000258info:eu-repo/semantics/altIdentifier/doi/10.1016/j.greeac.2022.100026info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:46:59Zoai:ri.conicet.gov.ar:11336/197933instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:46:59.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
title Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
spellingShingle Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
Elia Dazat, Ricardo Ariel
ENHANCED FLUORESCENCE
ERGOSTEROL
FOOD
GREEN CHEMISTRY
HYDROPHOBIC FLUORESCENT NATURAL DEEP EUTECTIC SOLVENTS
title_short Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
title_full Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
title_fullStr Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
title_full_unstemmed Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
title_sort Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent
dc.creator.none.fl_str_mv Elia Dazat, Ricardo Ariel
Mammana, Sabrina Belén
Canizo, Brenda Vanina
Silva, María Fernanda
Gomez, Federico Jose Vicente
author Elia Dazat, Ricardo Ariel
author_facet Elia Dazat, Ricardo Ariel
Mammana, Sabrina Belén
Canizo, Brenda Vanina
Silva, María Fernanda
Gomez, Federico Jose Vicente
author_role author
author2 Mammana, Sabrina Belén
Canizo, Brenda Vanina
Silva, María Fernanda
Gomez, Federico Jose Vicente
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENHANCED FLUORESCENCE
ERGOSTEROL
FOOD
GREEN CHEMISTRY
HYDROPHOBIC FLUORESCENT NATURAL DEEP EUTECTIC SOLVENTS
topic ENHANCED FLUORESCENCE
ERGOSTEROL
FOOD
GREEN CHEMISTRY
HYDROPHOBIC FLUORESCENT NATURAL DEEP EUTECTIC SOLVENTS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ergosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescent natural deep eutectic solvents (HPB-FLUODES) to determine ERG by fluorescence detection in edible mushrooms. The proposed approach involves a green sample preparation and a low cost instrumental technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis (2.4 samples per minute). The experimental variables, such as the type of HPB-FLUODES, sample/solvent mass/volume ratio, time, and temperature of extraction were studied and optimized. The selected variables were HPB-FLUODES thymol:lactic acid 1:2, 2.5 mg of sample, 1 mL of HPB-FLUODES, and 45 min of extraction at 40°C. The LOD and LOQ were 0.09 µg g−1 and 0.29 µg g−1, respectively. The results show an enhancement of ERG fluorescence signal of 1825% against the ERG signal in methanol media. The present approach shows outstanding green performance using AGREE®, AGREEprep® and WAC principles. Finally, the analysis of edible mushrooms demonstrates the feasibility of the proposed green methodology.
Fil: Elia Dazat, Ricardo Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Mammana, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Canizo, Brenda Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Silva, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Gomez, Federico Jose Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina
description Ergosterol (ERG) is a sterol found at high levels in cell membranes of fungi and is a smart option to be used as proof of fungal contamination in food samples. Therefore, the objective of this work was to develop a fast, sensitive and ecological methodology based on the use of hydrophobic fluorescent natural deep eutectic solvents (HPB-FLUODES) to determine ERG by fluorescence detection in edible mushrooms. The proposed approach involves a green sample preparation and a low cost instrumental technique leading to an efficient and simple analytical methodology suitable for high-throughput analysis (2.4 samples per minute). The experimental variables, such as the type of HPB-FLUODES, sample/solvent mass/volume ratio, time, and temperature of extraction were studied and optimized. The selected variables were HPB-FLUODES thymol:lactic acid 1:2, 2.5 mg of sample, 1 mL of HPB-FLUODES, and 45 min of extraction at 40°C. The LOD and LOQ were 0.09 µg g−1 and 0.29 µg g−1, respectively. The results show an enhancement of ERG fluorescence signal of 1825% against the ERG signal in methanol media. The present approach shows outstanding green performance using AGREE®, AGREEprep® and WAC principles. Finally, the analysis of edible mushrooms demonstrates the feasibility of the proposed green methodology.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/197933
Elia Dazat, Ricardo Ariel; Mammana, Sabrina Belén; Canizo, Brenda Vanina; Silva, María Fernanda; Gomez, Federico Jose Vicente; Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent; Elsevier Science; Green Analytical Chemistry; 3; 12-2022; 1-6
2772-5774
CONICET Digital
CONICET
url http://hdl.handle.net/11336/197933
identifier_str_mv Elia Dazat, Ricardo Ariel; Mammana, Sabrina Belén; Canizo, Brenda Vanina; Silva, María Fernanda; Gomez, Federico Jose Vicente; Enhanced fluorescence detection of ergosterol by hydrophobic fluorescent natural deep eutectic solvent; Elsevier Science; Green Analytical Chemistry; 3; 12-2022; 1-6
2772-5774
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2772577422000258
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.greeac.2022.100026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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