Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice

Autores
Trinchero, Mariela Fernanda; Koehl, Muriel; Bechakra, Malik; Delage, Pauline; Charrier, Vanessa; Grosjean, Noelle; Lavedeze, Elodie; Schinder, Alejandro Fabian; Abrous, D. Nora
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
New dentate granule cells (GCs) are generated in the hippocampus throughout life. These adult-born neurons are required for spatial learning in the Morris water maze (MWM). In rats, spatial learning shapes the network by regulating their number and dendritic development. Here, we explored whether such modulatory effects exist in mice. New GCs were tagged using thymidine analogs or a GFP-expressing retrovirus. Animals were exposed to a reference memory protocol for 10-14 days (spaced training) at different times after newborn cells labeling. Cell proliferation, cell survival, cell death, neuronal phenotype, and dendritic and spine development were examined using immunohistochemistry. Surprisingly, spatial learning did not modify any of the parameters under scrutiny including cell number and dendritic morphology. These results suggest that although new GCs are required in mice for spatial learning in the MWM, they are, at least for the developmental intervals analyzed here, refractory to behavioral stimuli generated in the course of learning in the MWM.
Fil: Trinchero, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Koehl, Muriel. Inserm; Francia
Fil: Bechakra, Malik. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Delage, Pauline. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Charrier, Vanessa. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Grosjean, Noelle. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Lavedeze, Elodie. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Schinder, Alejandro Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Abrous, D. Nora. Inserm; Francia. Universite de Bordeaux; Francia
Materia
Spatial learning
Adult neurogenesis
Morris Water Maze
Adult-born Neurons
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9758
Acceder
id CONICETDig_10479a784ae2e24e38c3678a1f277144
oai_identifier_str oai:ri.conicet.gov.ar:11336/9758
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of spaced learning in the water maze on development of dentate granule cells generated in adult miceTrinchero, Mariela FernandaKoehl, MurielBechakra, MalikDelage, PaulineCharrier, VanessaGrosjean, NoelleLavedeze, ElodieSchinder, Alejandro FabianAbrous, D. NoraSpatial learningAdult neurogenesisMorris Water MazeAdult-born Neuronshttps://purl.org/becyt/ford/3.5https://purl.org/becyt/ford/3New dentate granule cells (GCs) are generated in the hippocampus throughout life. These adult-born neurons are required for spatial learning in the Morris water maze (MWM). In rats, spatial learning shapes the network by regulating their number and dendritic development. Here, we explored whether such modulatory effects exist in mice. New GCs were tagged using thymidine analogs or a GFP-expressing retrovirus. Animals were exposed to a reference memory protocol for 10-14 days (spaced training) at different times after newborn cells labeling. Cell proliferation, cell survival, cell death, neuronal phenotype, and dendritic and spine development were examined using immunohistochemistry. Surprisingly, spatial learning did not modify any of the parameters under scrutiny including cell number and dendritic morphology. These results suggest that although new GCs are required in mice for spatial learning in the MWM, they are, at least for the developmental intervals analyzed here, refractory to behavioral stimuli generated in the course of learning in the MWM.Fil: Trinchero, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Koehl, Muriel. Inserm; FranciaFil: Bechakra, Malik. Inserm; Francia. Universite de Bordeaux; FranciaFil: Delage, Pauline. Inserm; Francia. Universite de Bordeaux; FranciaFil: Charrier, Vanessa. Inserm; Francia. Universite de Bordeaux; FranciaFil: Grosjean, Noelle. Inserm; Francia. Universite de Bordeaux; FranciaFil: Lavedeze, Elodie. Inserm; Francia. Universite de Bordeaux; FranciaFil: Schinder, Alejandro Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; ArgentinaFil: Abrous, D. Nora. Inserm; Francia. Universite de Bordeaux; FranciaWiley2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9758Trinchero, Mariela Fernanda; Koehl, Muriel; Bechakra, Malik; Delage, Pauline; Charrier, Vanessa; et al.; Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice; Wiley; Hippocampus; 25; 11; 11-2015; 1314-13261050-9631enginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22438/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.22438info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:13:45Zoai:ri.conicet.gov.ar:11336/9758instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:13:46.248CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
title Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
spellingShingle Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
Trinchero, Mariela Fernanda
Spatial learning
Adult neurogenesis
Morris Water Maze
Adult-born Neurons
title_short Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
title_full Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
title_fullStr Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
title_full_unstemmed Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
title_sort Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice
dc.creator.none.fl_str_mv Trinchero, Mariela Fernanda
Koehl, Muriel
Bechakra, Malik
Delage, Pauline
Charrier, Vanessa
Grosjean, Noelle
Lavedeze, Elodie
Schinder, Alejandro Fabian
Abrous, D. Nora
author Trinchero, Mariela Fernanda
author_facet Trinchero, Mariela Fernanda
Koehl, Muriel
Bechakra, Malik
Delage, Pauline
Charrier, Vanessa
Grosjean, Noelle
Lavedeze, Elodie
Schinder, Alejandro Fabian
Abrous, D. Nora
author_role author
author2 Koehl, Muriel
Bechakra, Malik
Delage, Pauline
Charrier, Vanessa
Grosjean, Noelle
Lavedeze, Elodie
Schinder, Alejandro Fabian
Abrous, D. Nora
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Spatial learning
Adult neurogenesis
Morris Water Maze
Adult-born Neurons
topic Spatial learning
Adult neurogenesis
Morris Water Maze
Adult-born Neurons
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.5
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv New dentate granule cells (GCs) are generated in the hippocampus throughout life. These adult-born neurons are required for spatial learning in the Morris water maze (MWM). In rats, spatial learning shapes the network by regulating their number and dendritic development. Here, we explored whether such modulatory effects exist in mice. New GCs were tagged using thymidine analogs or a GFP-expressing retrovirus. Animals were exposed to a reference memory protocol for 10-14 days (spaced training) at different times after newborn cells labeling. Cell proliferation, cell survival, cell death, neuronal phenotype, and dendritic and spine development were examined using immunohistochemistry. Surprisingly, spatial learning did not modify any of the parameters under scrutiny including cell number and dendritic morphology. These results suggest that although new GCs are required in mice for spatial learning in the MWM, they are, at least for the developmental intervals analyzed here, refractory to behavioral stimuli generated in the course of learning in the MWM.
Fil: Trinchero, Mariela Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Koehl, Muriel. Inserm; Francia
Fil: Bechakra, Malik. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Delage, Pauline. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Charrier, Vanessa. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Grosjean, Noelle. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Lavedeze, Elodie. Inserm; Francia. Universite de Bordeaux; Francia
Fil: Schinder, Alejandro Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquimicas de Buenos Aires; Argentina. Fundación Instituto Leloir; Argentina
Fil: Abrous, D. Nora. Inserm; Francia. Universite de Bordeaux; Francia
description New dentate granule cells (GCs) are generated in the hippocampus throughout life. These adult-born neurons are required for spatial learning in the Morris water maze (MWM). In rats, spatial learning shapes the network by regulating their number and dendritic development. Here, we explored whether such modulatory effects exist in mice. New GCs were tagged using thymidine analogs or a GFP-expressing retrovirus. Animals were exposed to a reference memory protocol for 10-14 days (spaced training) at different times after newborn cells labeling. Cell proliferation, cell survival, cell death, neuronal phenotype, and dendritic and spine development were examined using immunohistochemistry. Surprisingly, spatial learning did not modify any of the parameters under scrutiny including cell number and dendritic morphology. These results suggest that although new GCs are required in mice for spatial learning in the MWM, they are, at least for the developmental intervals analyzed here, refractory to behavioral stimuli generated in the course of learning in the MWM.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9758
Trinchero, Mariela Fernanda; Koehl, Muriel; Bechakra, Malik; Delage, Pauline; Charrier, Vanessa; et al.; Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice; Wiley; Hippocampus; 25; 11; 11-2015; 1314-1326
1050-9631
url http://hdl.handle.net/11336/9758
identifier_str_mv Trinchero, Mariela Fernanda; Koehl, Muriel; Bechakra, Malik; Delage, Pauline; Charrier, Vanessa; et al.; Effects of spaced learning in the water maze on development of dentate granule cells generated in adult mice; Wiley; Hippocampus; 25; 11; 11-2015; 1314-1326
1050-9631
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hipo.22438/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.22438
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980730304462848
score 12.993085

Publicaciones similares