Penicillamine determination using a tyrosinase micro-rotating biosensor

Autores
Torriero, Angel Alberto Jesus; Salinas, Eloy; Marchevsky, Eduardo Jorge; Raba, Julio; Silber, Juana J.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on glassy carbon electrode surface at -150 mV versus Ag/AgCl/NaCl 3 M. Thus, when penicillamine (PA) was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used for sensitive determination of PA in drug and human synthetic serum samples. A linear range of 0.02-80 μM (r = 0.999) was obtained for amperometric determination of PA in buffered pH 7.0 solutions (0.1 M phosphate buffer). The biosensor has a reasonable reproducibility (R.S.D. < 4.0%) and a very stable amperometric response toward this compound (more than 1 month).
Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silber, Juana J.. Universidad Nacional de Río Cuarto; Argentina
Materia
4-TERT-BUTYLCATECHOL
BIOSENSOR
GLASSY CARBON
PENICILLAMINE
TYROSINASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93547
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Penicillamine determination using a tyrosinase micro-rotating biosensorTorriero, Angel Alberto JesusSalinas, EloyMarchevsky, Eduardo JorgeRaba, JulioSilber, Juana J.4-TERT-BUTYLCATECHOLBIOSENSORGLASSY CARBONPENICILLAMINETYROSINASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on glassy carbon electrode surface at -150 mV versus Ag/AgCl/NaCl 3 M. Thus, when penicillamine (PA) was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used for sensitive determination of PA in drug and human synthetic serum samples. A linear range of 0.02-80 μM (r = 0.999) was obtained for amperometric determination of PA in buffered pH 7.0 solutions (0.1 M phosphate buffer). The biosensor has a reasonable reproducibility (R.S.D. < 4.0%) and a very stable amperometric response toward this compound (more than 1 month).Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; ArgentinaFil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Silber, Juana J.. Universidad Nacional de Río Cuarto; ArgentinaElsevier Science2006-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93547Torriero, Angel Alberto Jesus; Salinas, Eloy; Marchevsky, Eduardo Jorge; Raba, Julio; Silber, Juana J.; Penicillamine determination using a tyrosinase micro-rotating biosensor; Elsevier Science; Analytica Chimica Acta; 580; 2; 11-2006; 136-1420003-2670CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2006.07.067info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267006016448info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:10:26Zoai:ri.conicet.gov.ar:11336/93547instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:10:27.16CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Penicillamine determination using a tyrosinase micro-rotating biosensor
title Penicillamine determination using a tyrosinase micro-rotating biosensor
spellingShingle Penicillamine determination using a tyrosinase micro-rotating biosensor
Torriero, Angel Alberto Jesus
4-TERT-BUTYLCATECHOL
BIOSENSOR
GLASSY CARBON
PENICILLAMINE
TYROSINASE
title_short Penicillamine determination using a tyrosinase micro-rotating biosensor
title_full Penicillamine determination using a tyrosinase micro-rotating biosensor
title_fullStr Penicillamine determination using a tyrosinase micro-rotating biosensor
title_full_unstemmed Penicillamine determination using a tyrosinase micro-rotating biosensor
title_sort Penicillamine determination using a tyrosinase micro-rotating biosensor
dc.creator.none.fl_str_mv Torriero, Angel Alberto Jesus
Salinas, Eloy
Marchevsky, Eduardo Jorge
Raba, Julio
Silber, Juana J.
author Torriero, Angel Alberto Jesus
author_facet Torriero, Angel Alberto Jesus
Salinas, Eloy
Marchevsky, Eduardo Jorge
Raba, Julio
Silber, Juana J.
author_role author
author2 Salinas, Eloy
Marchevsky, Eduardo Jorge
Raba, Julio
Silber, Juana J.
author2_role author
author
author
author
dc.subject.none.fl_str_mv 4-TERT-BUTYLCATECHOL
BIOSENSOR
GLASSY CARBON
PENICILLAMINE
TYROSINASE
topic 4-TERT-BUTYLCATECHOL
BIOSENSOR
GLASSY CARBON
PENICILLAMINE
TYROSINASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on glassy carbon electrode surface at -150 mV versus Ag/AgCl/NaCl 3 M. Thus, when penicillamine (PA) was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used for sensitive determination of PA in drug and human synthetic serum samples. A linear range of 0.02-80 μM (r = 0.999) was obtained for amperometric determination of PA in buffered pH 7.0 solutions (0.1 M phosphate buffer). The biosensor has a reasonable reproducibility (R.S.D. < 4.0%) and a very stable amperometric response toward this compound (more than 1 month).
Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Marchevsky, Eduardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silber, Juana J.. Universidad Nacional de Río Cuarto; Argentina
description Tyrosinase [EC 1.14.18.1], immobilized on a rotating disk, catalyzed the oxidation of catechols to o-benzoquinone, whose back electrochemical reduction was detected on glassy carbon electrode surface at -150 mV versus Ag/AgCl/NaCl 3 M. Thus, when penicillamine (PA) was added to the solution, this thiol-containing compound participate in Michael type addition reactions with o-benzoquinone to form the corresponding thioquinone derivatives, decreasing the reduction current obtained proportionally to the increase of its concentration. This method could be used for sensitive determination of PA in drug and human synthetic serum samples. A linear range of 0.02-80 μM (r = 0.999) was obtained for amperometric determination of PA in buffered pH 7.0 solutions (0.1 M phosphate buffer). The biosensor has a reasonable reproducibility (R.S.D. < 4.0%) and a very stable amperometric response toward this compound (more than 1 month).
publishDate 2006
dc.date.none.fl_str_mv 2006-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93547
Torriero, Angel Alberto Jesus; Salinas, Eloy; Marchevsky, Eduardo Jorge; Raba, Julio; Silber, Juana J.; Penicillamine determination using a tyrosinase micro-rotating biosensor; Elsevier Science; Analytica Chimica Acta; 580; 2; 11-2006; 136-142
0003-2670
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93547
identifier_str_mv Torriero, Angel Alberto Jesus; Salinas, Eloy; Marchevsky, Eduardo Jorge; Raba, Julio; Silber, Juana J.; Penicillamine determination using a tyrosinase micro-rotating biosensor; Elsevier Science; Analytica Chimica Acta; 580; 2; 11-2006; 136-142
0003-2670
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.aca.2006.07.067
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0003267006016448
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980526126792704
score 12.993085

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