Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples

Autores
Varmira, Kambiz; Mohammadi, Ghobad; Mahmoudi, Majid; Khodarahmi, Reza; Rashidi, Khodabakhsh; Hedayati, Mehdi; Goicoechea, Hector Casimiro; Jalalvand, Ali R.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this work, fabrication of a novel and ultrasensitive electrochemical biosensor based on immobilization oftyrosine hydroxylase onto palladium-platinum bimetallic alloy nanoparticles/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide/graphene-multiwalled carbon nanotubes-IL/glassycarbon electrode for determination of L-tyrosine in some high tyrosine foods including cheese, egg and yogurtwas reported. Immobilization of tyrosine hydroxylase onto the surface of the biosensor was performed bycross-linking tyrosine hydroxylase and chitosan through the addition of glutaraldehyde. Enzymatic biosensorsemploy the affinity and selectivity of catalytically active proteins towards their target molecules and here, thetyrosine hydroxylase selectively catalyzes the conversion of tyrosine to levodopa which can be oxidized atlower potentials than tyrosine. The modifications were characterized by electrochemical impedancespectroscopy, cyclic voltammetry, energy dispersive X-ray spectroscopic and scanning electron microscopy.Under optimal conditions, the biosensor detected tyrosine in concentration ranges of 0.01×10-9 to 8.0×10-9 molL-1 and 8.0×10-9 to 160.0×10-9 mol L-1 with a limit of detection of 0.009×10-9 mol L-1. The biosensor was ableto selective determination of tyrosine even in the presence of common interferents therefore, the biosensor washighly selective. The biosensor also showed good operational stability, antifouling properties, sensitivity,repeatability and reproducibility.
Fil: Varmira, Kambiz. Kermanshah University Of Medical Sciences; Irán
Fil: Mohammadi, Ghobad. Kermanshah University Of Medical Sciences; Irán
Fil: Mahmoudi, Majid. Kermanshah University Of Medical Sciences; Irán
Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán
Fil: Rashidi, Khodabakhsh. Kermanshah University Of Medical Sciences; Irán
Fil: Hedayati, Mehdi. The Cellular And Molecular Endocrine Research Center; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
Materia
Tyrosine
High tyrosine foods
Levodopa
Biosensor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/89243
Acceder
id CONICETDig_6aa1aa29ecea46892abb60e072806a7c
oai_identifier_str oai:ri.conicet.gov.ar:11336/89243
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samplesVarmira, KambizMohammadi, GhobadMahmoudi, MajidKhodarahmi, RezaRashidi, KhodabakhshHedayati, MehdiGoicoechea, Hector CasimiroJalalvand, Ali R.TyrosineHigh tyrosine foodsLevodopaBiosensorhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1In this work, fabrication of a novel and ultrasensitive electrochemical biosensor based on immobilization oftyrosine hydroxylase onto palladium-platinum bimetallic alloy nanoparticles/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide/graphene-multiwalled carbon nanotubes-IL/glassycarbon electrode for determination of L-tyrosine in some high tyrosine foods including cheese, egg and yogurtwas reported. Immobilization of tyrosine hydroxylase onto the surface of the biosensor was performed bycross-linking tyrosine hydroxylase and chitosan through the addition of glutaraldehyde. Enzymatic biosensorsemploy the affinity and selectivity of catalytically active proteins towards their target molecules and here, thetyrosine hydroxylase selectively catalyzes the conversion of tyrosine to levodopa which can be oxidized atlower potentials than tyrosine. The modifications were characterized by electrochemical impedancespectroscopy, cyclic voltammetry, energy dispersive X-ray spectroscopic and scanning electron microscopy.Under optimal conditions, the biosensor detected tyrosine in concentration ranges of 0.01×10-9 to 8.0×10-9 molL-1 and 8.0×10-9 to 160.0×10-9 mol L-1 with a limit of detection of 0.009×10-9 mol L-1. The biosensor was ableto selective determination of tyrosine even in the presence of common interferents therefore, the biosensor washighly selective. The biosensor also showed good operational stability, antifouling properties, sensitivity,repeatability and reproducibility.Fil: Varmira, Kambiz. Kermanshah University Of Medical Sciences; IránFil: Mohammadi, Ghobad. Kermanshah University Of Medical Sciences; IránFil: Mahmoudi, Majid. Kermanshah University Of Medical Sciences; IránFil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; IránFil: Rashidi, Khodabakhsh. Kermanshah University Of Medical Sciences; IránFil: Hedayati, Mehdi. The Cellular And Molecular Endocrine Research Center; IránFil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; ArgentinaFil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; IránElsevier Science2018-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/89243Varmira, Kambiz; Mohammadi, Ghobad; Mahmoudi, Majid; Khodarahmi, Reza; Rashidi, Khodabakhsh; et al.; Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples; Elsevier Science; Talanta; 183; 6-2018; 1-100039-9140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0039914018301619info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2018.02.053info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:22:19Zoai:ri.conicet.gov.ar:11336/89243instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:22:20.001CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
title Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
spellingShingle Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
Varmira, Kambiz
Tyrosine
High tyrosine foods
Levodopa
Biosensor
title_short Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
title_full Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
title_fullStr Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
title_full_unstemmed Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
title_sort Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples
dc.creator.none.fl_str_mv Varmira, Kambiz
Mohammadi, Ghobad
Mahmoudi, Majid
Khodarahmi, Reza
Rashidi, Khodabakhsh
Hedayati, Mehdi
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author Varmira, Kambiz
author_facet Varmira, Kambiz
Mohammadi, Ghobad
Mahmoudi, Majid
Khodarahmi, Reza
Rashidi, Khodabakhsh
Hedayati, Mehdi
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author_role author
author2 Mohammadi, Ghobad
Mahmoudi, Majid
Khodarahmi, Reza
Rashidi, Khodabakhsh
Hedayati, Mehdi
Goicoechea, Hector Casimiro
Jalalvand, Ali R.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tyrosine
High tyrosine foods
Levodopa
Biosensor
topic Tyrosine
High tyrosine foods
Levodopa
Biosensor
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this work, fabrication of a novel and ultrasensitive electrochemical biosensor based on immobilization oftyrosine hydroxylase onto palladium-platinum bimetallic alloy nanoparticles/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide/graphene-multiwalled carbon nanotubes-IL/glassycarbon electrode for determination of L-tyrosine in some high tyrosine foods including cheese, egg and yogurtwas reported. Immobilization of tyrosine hydroxylase onto the surface of the biosensor was performed bycross-linking tyrosine hydroxylase and chitosan through the addition of glutaraldehyde. Enzymatic biosensorsemploy the affinity and selectivity of catalytically active proteins towards their target molecules and here, thetyrosine hydroxylase selectively catalyzes the conversion of tyrosine to levodopa which can be oxidized atlower potentials than tyrosine. The modifications were characterized by electrochemical impedancespectroscopy, cyclic voltammetry, energy dispersive X-ray spectroscopic and scanning electron microscopy.Under optimal conditions, the biosensor detected tyrosine in concentration ranges of 0.01×10-9 to 8.0×10-9 molL-1 and 8.0×10-9 to 160.0×10-9 mol L-1 with a limit of detection of 0.009×10-9 mol L-1. The biosensor was ableto selective determination of tyrosine even in the presence of common interferents therefore, the biosensor washighly selective. The biosensor also showed good operational stability, antifouling properties, sensitivity,repeatability and reproducibility.
Fil: Varmira, Kambiz. Kermanshah University Of Medical Sciences; Irán
Fil: Mohammadi, Ghobad. Kermanshah University Of Medical Sciences; Irán
Fil: Mahmoudi, Majid. Kermanshah University Of Medical Sciences; Irán
Fil: Khodarahmi, Reza. Kermanshah University Of Medical Sciences; Irán
Fil: Rashidi, Khodabakhsh. Kermanshah University Of Medical Sciences; Irán
Fil: Hedayati, Mehdi. The Cellular And Molecular Endocrine Research Center; Irán
Fil: Goicoechea, Hector Casimiro. Universidad Nacional del Litoral; Argentina
Fil: Jalalvand, Ali R.. Kermanshah University Of Medical Sciences; Irán
description In this work, fabrication of a novel and ultrasensitive electrochemical biosensor based on immobilization oftyrosine hydroxylase onto palladium-platinum bimetallic alloy nanoparticles/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide/graphene-multiwalled carbon nanotubes-IL/glassycarbon electrode for determination of L-tyrosine in some high tyrosine foods including cheese, egg and yogurtwas reported. Immobilization of tyrosine hydroxylase onto the surface of the biosensor was performed bycross-linking tyrosine hydroxylase and chitosan through the addition of glutaraldehyde. Enzymatic biosensorsemploy the affinity and selectivity of catalytically active proteins towards their target molecules and here, thetyrosine hydroxylase selectively catalyzes the conversion of tyrosine to levodopa which can be oxidized atlower potentials than tyrosine. The modifications were characterized by electrochemical impedancespectroscopy, cyclic voltammetry, energy dispersive X-ray spectroscopic and scanning electron microscopy.Under optimal conditions, the biosensor detected tyrosine in concentration ranges of 0.01×10-9 to 8.0×10-9 molL-1 and 8.0×10-9 to 160.0×10-9 mol L-1 with a limit of detection of 0.009×10-9 mol L-1. The biosensor was ableto selective determination of tyrosine even in the presence of common interferents therefore, the biosensor washighly selective. The biosensor also showed good operational stability, antifouling properties, sensitivity,repeatability and reproducibility.
publishDate 2018
dc.date.none.fl_str_mv 2018-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/89243
Varmira, Kambiz; Mohammadi, Ghobad; Mahmoudi, Majid; Khodarahmi, Reza; Rashidi, Khodabakhsh; et al.; Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples; Elsevier Science; Talanta; 183; 6-2018; 1-10
0039-9140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/89243
identifier_str_mv Varmira, Kambiz; Mohammadi, Ghobad; Mahmoudi, Majid; Khodarahmi, Reza; Rashidi, Khodabakhsh; et al.; Fabrication of a novel enzymatic electrochemical biosensor for determination of tyrosine in some food samples; Elsevier Science; Talanta; 183; 6-2018; 1-10
0039-9140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0039914018301619
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2018.02.053
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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