Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor

Autores
Torriero, Angel Alberto Jesus; Salinas, Eloy; Raba, Julio; Silber, Juana
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The high sensitivity that can be attained using an enzymatic system and mediated by catechol has been verified by on-line interfacing of a rotating biosensor and continuous-flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP, [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide catalyzed the oxidation of catechol, whose back electrochemical reduction was detected on glassy carbon electrode surface at −200mV. Thus, when ciprofloxacin (CF) or norfloxacin (NF) was added to the solution, these piperazine-containing compounds participate in Michael addition reactions with catechol to form the corresponding aminoquinone derivative, decreasing the peak current obtained in proportion with the increase of its concentration. CFwas used as the model piperazine-containing compound for the study. The influenceof indicator composition on the nature of the analytical response has been assessed through examining the electrochemical properties of three derivatives. Interference by electroactive species (ascorbate, urate, and tyrosine) and other physiological constituents (cysteine, glutathione) has also been assessed.
Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química Analítica; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química Analítica; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silber, Juana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
Materia
BIOSENSOR
CATECHOL
CIPROFLOXACIN
FIA
GLASSY CARBON
NORFLOXACIN
PEROXIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94295
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensorTorriero, Angel Alberto JesusSalinas, EloyRaba, JulioSilber, JuanaBIOSENSORCATECHOLCIPROFLOXACINFIAGLASSY CARBONNORFLOXACINPEROXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The high sensitivity that can be attained using an enzymatic system and mediated by catechol has been verified by on-line interfacing of a rotating biosensor and continuous-flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP, [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide catalyzed the oxidation of catechol, whose back electrochemical reduction was detected on glassy carbon electrode surface at −200mV. Thus, when ciprofloxacin (CF) or norfloxacin (NF) was added to the solution, these piperazine-containing compounds participate in Michael addition reactions with catechol to form the corresponding aminoquinone derivative, decreasing the peak current obtained in proportion with the increase of its concentration. CFwas used as the model piperazine-containing compound for the study. The influenceof indicator composition on the nature of the analytical response has been assessed through examining the electrochemical properties of three derivatives. Interference by electroactive species (ascorbate, urate, and tyrosine) and other physiological constituents (cysteine, glutathione) has also been assessed.Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química Analítica; ArgentinaFil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química Analítica; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Silber, Juana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; ArgentinaElsevier Advanced Technology2006-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94295Torriero, Angel Alberto Jesus; Salinas, Eloy; Raba, Julio; Silber, Juana; Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor; Elsevier Advanced Technology; Biosensors & Bioelectronics; 22; 1; 7-2006; 109-1150956-5663CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566305003994info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2005.12.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:23Zoai:ri.conicet.gov.ar:11336/94295instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:23.444CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
title Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
spellingShingle Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
Torriero, Angel Alberto Jesus
BIOSENSOR
CATECHOL
CIPROFLOXACIN
FIA
GLASSY CARBON
NORFLOXACIN
PEROXIDASE
title_short Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
title_full Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
title_fullStr Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
title_full_unstemmed Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
title_sort Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor
dc.creator.none.fl_str_mv Torriero, Angel Alberto Jesus
Salinas, Eloy
Raba, Julio
Silber, Juana
author Torriero, Angel Alberto Jesus
author_facet Torriero, Angel Alberto Jesus
Salinas, Eloy
Raba, Julio
Silber, Juana
author_role author
author2 Salinas, Eloy
Raba, Julio
Silber, Juana
author2_role author
author
author
dc.subject.none.fl_str_mv BIOSENSOR
CATECHOL
CIPROFLOXACIN
FIA
GLASSY CARBON
NORFLOXACIN
PEROXIDASE
topic BIOSENSOR
CATECHOL
CIPROFLOXACIN
FIA
GLASSY CARBON
NORFLOXACIN
PEROXIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The high sensitivity that can be attained using an enzymatic system and mediated by catechol has been verified by on-line interfacing of a rotating biosensor and continuous-flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP, [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide catalyzed the oxidation of catechol, whose back electrochemical reduction was detected on glassy carbon electrode surface at −200mV. Thus, when ciprofloxacin (CF) or norfloxacin (NF) was added to the solution, these piperazine-containing compounds participate in Michael addition reactions with catechol to form the corresponding aminoquinone derivative, decreasing the peak current obtained in proportion with the increase of its concentration. CFwas used as the model piperazine-containing compound for the study. The influenceof indicator composition on the nature of the analytical response has been assessed through examining the electrochemical properties of three derivatives. Interference by electroactive species (ascorbate, urate, and tyrosine) and other physiological constituents (cysteine, glutathione) has also been assessed.
Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química Analítica; Argentina
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química Analítica; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silber, Juana. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina
description The high sensitivity that can be attained using an enzymatic system and mediated by catechol has been verified by on-line interfacing of a rotating biosensor and continuous-flow/stopped-flow/continuous-flow processing. Horseradish peroxidase, HRP, [EC 1.11.1.7], immobilized on a rotating disk, in the presence of hydrogen peroxide catalyzed the oxidation of catechol, whose back electrochemical reduction was detected on glassy carbon electrode surface at −200mV. Thus, when ciprofloxacin (CF) or norfloxacin (NF) was added to the solution, these piperazine-containing compounds participate in Michael addition reactions with catechol to form the corresponding aminoquinone derivative, decreasing the peak current obtained in proportion with the increase of its concentration. CFwas used as the model piperazine-containing compound for the study. The influenceof indicator composition on the nature of the analytical response has been assessed through examining the electrochemical properties of three derivatives. Interference by electroactive species (ascorbate, urate, and tyrosine) and other physiological constituents (cysteine, glutathione) has also been assessed.
publishDate 2006
dc.date.none.fl_str_mv 2006-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94295
Torriero, Angel Alberto Jesus; Salinas, Eloy; Raba, Julio; Silber, Juana; Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor; Elsevier Advanced Technology; Biosensors & Bioelectronics; 22; 1; 7-2006; 109-115
0956-5663
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94295
identifier_str_mv Torriero, Angel Alberto Jesus; Salinas, Eloy; Raba, Julio; Silber, Juana; Sensitive determination of ciprofloxacin and norfloxacin in biological fluids using an enzymatic rotating biosensor; Elsevier Advanced Technology; Biosensors & Bioelectronics; 22; 1; 7-2006; 109-115
0956-5663
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0956566305003994
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bios.2005.12.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Advanced Technology
publisher.none.fl_str_mv Elsevier Advanced Technology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083553941520384
score 13.22299

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