Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system

Autores
Salinas, Eloy; Rivero Osimani, Valeria Leticia; Torriero, Angel Alberto Jesus; Benuzzi, Delia Aurora; Sanz Ferramola, Maria Isabel; Raba, Julio
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fabrication of an amperometric-rotating biosensor for the enzymatic determination of cholesterol is reported. The assay utilizes a combination of three enzymes: cholesterol esterase (ChE), cholesterol oxidase (ChOx) and peroxidase (HRP); which were co-immobilizing on a rotatory disk. The method is developed by the use of a glassy carbon electrode as detector versus Ag/AgCl/3 M NaCl in conjunction with a soluble-redox mediator 4-tert-butylcatechol (TBC). ChE converts esterified cholesterol to free cholesterol, which is then oxidized by ChOx with hydrogen peroxide as product. TBC is converted to 4-tert-butylbenzoquinone (TBB) by hydrogen peroxide, catalyzed by HRP, and the glassy carbon electrode responds to the TBB concentration. The system has integrated a micro packed-column with immobilized ascorbate oxidase (AAOx) that works as prereactor to eliminate l-ascorbic acid (AA) interference. This method could be used to determine total cholesterol concentration in the range 1.2 μM-1 mM (r = 0.999). A fast response time of 2 min has been observed with this amperometric-rotating biosensor. Lifetime is up to 25 days of use. The calculated detection limits was 11.9 nM. Reproducibility assays were made using repetitive standards solutions (n = 5) and the percentage standard error was less than 4%.
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Rivero Osimani, Valeria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Benuzzi, Delia Aurora. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
CHOLESTEROL
CHOLESTEROL ESTERASE
CHOLESTEROL OXIDASE
FIA
GLASSY CARBON ELECTRODE
HORSERADISH PEROXIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/93230
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/93230
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Multienzymatic-rotating biosensor for total cholesterol determination in a FIA systemSalinas, EloyRivero Osimani, Valeria LeticiaTorriero, Angel Alberto JesusBenuzzi, Delia AuroraSanz Ferramola, Maria IsabelRaba, JulioCHOLESTEROLCHOLESTEROL ESTERASECHOLESTEROL OXIDASEFIAGLASSY CARBON ELECTRODEHORSERADISH PEROXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Fabrication of an amperometric-rotating biosensor for the enzymatic determination of cholesterol is reported. The assay utilizes a combination of three enzymes: cholesterol esterase (ChE), cholesterol oxidase (ChOx) and peroxidase (HRP); which were co-immobilizing on a rotatory disk. The method is developed by the use of a glassy carbon electrode as detector versus Ag/AgCl/3 M NaCl in conjunction with a soluble-redox mediator 4-tert-butylcatechol (TBC). ChE converts esterified cholesterol to free cholesterol, which is then oxidized by ChOx with hydrogen peroxide as product. TBC is converted to 4-tert-butylbenzoquinone (TBB) by hydrogen peroxide, catalyzed by HRP, and the glassy carbon electrode responds to the TBB concentration. The system has integrated a micro packed-column with immobilized ascorbate oxidase (AAOx) that works as prereactor to eliminate l-ascorbic acid (AA) interference. This method could be used to determine total cholesterol concentration in the range 1.2 μM-1 mM (r = 0.999). A fast response time of 2 min has been observed with this amperometric-rotating biosensor. Lifetime is up to 25 days of use. The calculated detection limits was 11.9 nM. Reproducibility assays were made using repetitive standards solutions (n = 5) and the percentage standard error was less than 4%.Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; ArgentinaFil: Rivero Osimani, Valeria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Benuzzi, Delia Aurora. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; ArgentinaFil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaElsevier Science2006-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/93230Salinas, Eloy; Rivero Osimani, Valeria Leticia; Torriero, Angel Alberto Jesus; Benuzzi, Delia Aurora; Sanz Ferramola, Maria Isabel; et al.; Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system; Elsevier Science; Talanta; 70; 2; 9-2006; 244-2500039-9140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2006.02.043info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0039914006001639info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:04:28Zoai:ri.conicet.gov.ar:11336/93230instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:04:28.4CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
title Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
spellingShingle Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
Salinas, Eloy
CHOLESTEROL
CHOLESTEROL ESTERASE
CHOLESTEROL OXIDASE
FIA
GLASSY CARBON ELECTRODE
HORSERADISH PEROXIDASE
title_short Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
title_full Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
title_fullStr Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
title_full_unstemmed Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
title_sort Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system
dc.creator.none.fl_str_mv Salinas, Eloy
Rivero Osimani, Valeria Leticia
Torriero, Angel Alberto Jesus
Benuzzi, Delia Aurora
Sanz Ferramola, Maria Isabel
Raba, Julio
author Salinas, Eloy
author_facet Salinas, Eloy
Rivero Osimani, Valeria Leticia
Torriero, Angel Alberto Jesus
Benuzzi, Delia Aurora
Sanz Ferramola, Maria Isabel
Raba, Julio
author_role author
author2 Rivero Osimani, Valeria Leticia
Torriero, Angel Alberto Jesus
Benuzzi, Delia Aurora
Sanz Ferramola, Maria Isabel
Raba, Julio
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CHOLESTEROL
CHOLESTEROL ESTERASE
CHOLESTEROL OXIDASE
FIA
GLASSY CARBON ELECTRODE
HORSERADISH PEROXIDASE
topic CHOLESTEROL
CHOLESTEROL ESTERASE
CHOLESTEROL OXIDASE
FIA
GLASSY CARBON ELECTRODE
HORSERADISH PEROXIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fabrication of an amperometric-rotating biosensor for the enzymatic determination of cholesterol is reported. The assay utilizes a combination of three enzymes: cholesterol esterase (ChE), cholesterol oxidase (ChOx) and peroxidase (HRP); which were co-immobilizing on a rotatory disk. The method is developed by the use of a glassy carbon electrode as detector versus Ag/AgCl/3 M NaCl in conjunction with a soluble-redox mediator 4-tert-butylcatechol (TBC). ChE converts esterified cholesterol to free cholesterol, which is then oxidized by ChOx with hydrogen peroxide as product. TBC is converted to 4-tert-butylbenzoquinone (TBB) by hydrogen peroxide, catalyzed by HRP, and the glassy carbon electrode responds to the TBB concentration. The system has integrated a micro packed-column with immobilized ascorbate oxidase (AAOx) that works as prereactor to eliminate l-ascorbic acid (AA) interference. This method could be used to determine total cholesterol concentration in the range 1.2 μM-1 mM (r = 0.999). A fast response time of 2 min has been observed with this amperometric-rotating biosensor. Lifetime is up to 25 days of use. The calculated detection limits was 11.9 nM. Reproducibility assays were made using repetitive standards solutions (n = 5) and the percentage standard error was less than 4%.
Fil: Salinas, Eloy. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Rivero Osimani, Valeria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Torriero, Angel Alberto Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Benuzzi, Delia Aurora. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description Fabrication of an amperometric-rotating biosensor for the enzymatic determination of cholesterol is reported. The assay utilizes a combination of three enzymes: cholesterol esterase (ChE), cholesterol oxidase (ChOx) and peroxidase (HRP); which were co-immobilizing on a rotatory disk. The method is developed by the use of a glassy carbon electrode as detector versus Ag/AgCl/3 M NaCl in conjunction with a soluble-redox mediator 4-tert-butylcatechol (TBC). ChE converts esterified cholesterol to free cholesterol, which is then oxidized by ChOx with hydrogen peroxide as product. TBC is converted to 4-tert-butylbenzoquinone (TBB) by hydrogen peroxide, catalyzed by HRP, and the glassy carbon electrode responds to the TBB concentration. The system has integrated a micro packed-column with immobilized ascorbate oxidase (AAOx) that works as prereactor to eliminate l-ascorbic acid (AA) interference. This method could be used to determine total cholesterol concentration in the range 1.2 μM-1 mM (r = 0.999). A fast response time of 2 min has been observed with this amperometric-rotating biosensor. Lifetime is up to 25 days of use. The calculated detection limits was 11.9 nM. Reproducibility assays were made using repetitive standards solutions (n = 5) and the percentage standard error was less than 4%.
publishDate 2006
dc.date.none.fl_str_mv 2006-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/93230
Salinas, Eloy; Rivero Osimani, Valeria Leticia; Torriero, Angel Alberto Jesus; Benuzzi, Delia Aurora; Sanz Ferramola, Maria Isabel; et al.; Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system; Elsevier Science; Talanta; 70; 2; 9-2006; 244-250
0039-9140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/93230
identifier_str_mv Salinas, Eloy; Rivero Osimani, Valeria Leticia; Torriero, Angel Alberto Jesus; Benuzzi, Delia Aurora; Sanz Ferramola, Maria Isabel; et al.; Multienzymatic-rotating biosensor for total cholesterol determination in a FIA system; Elsevier Science; Talanta; 70; 2; 9-2006; 244-250
0039-9140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2006.02.043
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0039914006001639
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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