NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies

Autores
Ochocinska, Margaret J.; Muñoz, Estela Maris; Veleri, Shobi; Weller, Joan L.; Coon, Steven L.; Pozdeyev, Nikita; Iuvone, P. Michael; Goebbels, Sandra; Furukawa, Takahisa; Klein, David C.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
NeuroD1 encodes a basic helix-loop-helix transcription factor involved in the development of neural and endocrine structures, including the retina and pineal gland. To determine the effect of NeuroD1 knockout in these tissues, a Cre/loxP recombination strategy was used to target a NeuroD1 floxed gene and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of Crx, which is selectively expressed in the pineal gland and retina. At 2 months of age, NeuroD1 cKO retinas have a dramatic reduction in rod- and cone-driven electroretinograms and contain shortened and disorganized outer segments; by 4 months, NeuroD1 cKO retinas are devoid of photoreceptors. In contrast, the NeuroD1 cKO pineal gland appears histologically normal. Microarray analysis of 2-month-old NeuroD1 cKO retina and pineal gland identified a subset of genes that were affected 2-100-fold; in addition, a small group of genes exhibit altered differential night/day expression. Included in the down-regulated genes are Aipl1, which is necessary to prevent retinal degeneration, and Ankrd33, whose protein product is selectively expressed in the outer segments. These findings suggest that NeuroD1 may act through Aipl1 and other genes to maintain photoreceptor homeostasis.
Fil: Ochocinska, Margaret J.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Veleri, Shobi. National Institutes of Health; Estados Unidos
Fil: Weller, Joan L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Pozdeyev, Nikita. University of Emory; Estados Unidos
Fil: Iuvone, P. Michael. University of Emory; Estados Unidos
Fil: Goebbels, Sandra. Max Planck Institute of Experimental Medicine; Alemania
Fil: Furukawa, Takahisa. Osaka University; Japón
Fil: Klein, David C.. National Instituto of Child Health & Human Development; Estados Unidos
Materia
GENE EXPRESSION
MICROARRAY
NEUROD1
PINEAL GLAND
RETINA
TRANSCRIPTOME PROFILING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/142317
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/142317
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studiesOchocinska, Margaret J.Muñoz, Estela MarisVeleri, ShobiWeller, Joan L.Coon, Steven L.Pozdeyev, NikitaIuvone, P. MichaelGoebbels, SandraFurukawa, TakahisaKlein, David C.GENE EXPRESSIONMICROARRAYNEUROD1PINEAL GLANDRETINATRANSCRIPTOME PROFILINGhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1NeuroD1 encodes a basic helix-loop-helix transcription factor involved in the development of neural and endocrine structures, including the retina and pineal gland. To determine the effect of NeuroD1 knockout in these tissues, a Cre/loxP recombination strategy was used to target a NeuroD1 floxed gene and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of Crx, which is selectively expressed in the pineal gland and retina. At 2 months of age, NeuroD1 cKO retinas have a dramatic reduction in rod- and cone-driven electroretinograms and contain shortened and disorganized outer segments; by 4 months, NeuroD1 cKO retinas are devoid of photoreceptors. In contrast, the NeuroD1 cKO pineal gland appears histologically normal. Microarray analysis of 2-month-old NeuroD1 cKO retina and pineal gland identified a subset of genes that were affected 2-100-fold; in addition, a small group of genes exhibit altered differential night/day expression. Included in the down-regulated genes are Aipl1, which is necessary to prevent retinal degeneration, and Ankrd33, whose protein product is selectively expressed in the outer segments. These findings suggest that NeuroD1 may act through Aipl1 and other genes to maintain photoreceptor homeostasis.Fil: Ochocinska, Margaret J.. National Instituto of Child Health & Human Development; Estados UnidosFil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Veleri, Shobi. National Institutes of Health; Estados UnidosFil: Weller, Joan L.. National Instituto of Child Health & Human Development; Estados UnidosFil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados UnidosFil: Pozdeyev, Nikita. University of Emory; Estados UnidosFil: Iuvone, P. Michael. University of Emory; Estados UnidosFil: Goebbels, Sandra. Max Planck Institute of Experimental Medicine; AlemaniaFil: Furukawa, Takahisa. Osaka University; JapónFil: Klein, David C.. National Instituto of Child Health & Human Development; Estados UnidosWiley Blackwell Publishing, Inc2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/142317Ochocinska, Margaret J.; Muñoz, Estela Maris; Veleri, Shobi; Weller, Joan L.; Coon, Steven L.; et al.; NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 123; 1; 10-2012; 44-590022-3042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2012.07870.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07870.xinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441145/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:40:49Zoai:ri.conicet.gov.ar:11336/142317instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:40:49.898CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
title NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
spellingShingle NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
Ochocinska, Margaret J.
GENE EXPRESSION
MICROARRAY
NEUROD1
PINEAL GLAND
RETINA
TRANSCRIPTOME PROFILING
title_short NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
title_full NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
title_fullStr NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
title_full_unstemmed NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
title_sort NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies
dc.creator.none.fl_str_mv Ochocinska, Margaret J.
Muñoz, Estela Maris
Veleri, Shobi
Weller, Joan L.
Coon, Steven L.
Pozdeyev, Nikita
Iuvone, P. Michael
Goebbels, Sandra
Furukawa, Takahisa
Klein, David C.
author Ochocinska, Margaret J.
author_facet Ochocinska, Margaret J.
Muñoz, Estela Maris
Veleri, Shobi
Weller, Joan L.
Coon, Steven L.
Pozdeyev, Nikita
Iuvone, P. Michael
Goebbels, Sandra
Furukawa, Takahisa
Klein, David C.
author_role author
author2 Muñoz, Estela Maris
Veleri, Shobi
Weller, Joan L.
Coon, Steven L.
Pozdeyev, Nikita
Iuvone, P. Michael
Goebbels, Sandra
Furukawa, Takahisa
Klein, David C.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GENE EXPRESSION
MICROARRAY
NEUROD1
PINEAL GLAND
RETINA
TRANSCRIPTOME PROFILING
topic GENE EXPRESSION
MICROARRAY
NEUROD1
PINEAL GLAND
RETINA
TRANSCRIPTOME PROFILING
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv NeuroD1 encodes a basic helix-loop-helix transcription factor involved in the development of neural and endocrine structures, including the retina and pineal gland. To determine the effect of NeuroD1 knockout in these tissues, a Cre/loxP recombination strategy was used to target a NeuroD1 floxed gene and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of Crx, which is selectively expressed in the pineal gland and retina. At 2 months of age, NeuroD1 cKO retinas have a dramatic reduction in rod- and cone-driven electroretinograms and contain shortened and disorganized outer segments; by 4 months, NeuroD1 cKO retinas are devoid of photoreceptors. In contrast, the NeuroD1 cKO pineal gland appears histologically normal. Microarray analysis of 2-month-old NeuroD1 cKO retina and pineal gland identified a subset of genes that were affected 2-100-fold; in addition, a small group of genes exhibit altered differential night/day expression. Included in the down-regulated genes are Aipl1, which is necessary to prevent retinal degeneration, and Ankrd33, whose protein product is selectively expressed in the outer segments. These findings suggest that NeuroD1 may act through Aipl1 and other genes to maintain photoreceptor homeostasis.
Fil: Ochocinska, Margaret J.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Veleri, Shobi. National Institutes of Health; Estados Unidos
Fil: Weller, Joan L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Pozdeyev, Nikita. University of Emory; Estados Unidos
Fil: Iuvone, P. Michael. University of Emory; Estados Unidos
Fil: Goebbels, Sandra. Max Planck Institute of Experimental Medicine; Alemania
Fil: Furukawa, Takahisa. Osaka University; Japón
Fil: Klein, David C.. National Instituto of Child Health & Human Development; Estados Unidos
description NeuroD1 encodes a basic helix-loop-helix transcription factor involved in the development of neural and endocrine structures, including the retina and pineal gland. To determine the effect of NeuroD1 knockout in these tissues, a Cre/loxP recombination strategy was used to target a NeuroD1 floxed gene and generate NeuroD1 conditional knockout (cKO) mice. Tissue specificity was conferred using Cre recombinase expressed under the control of the promoter of Crx, which is selectively expressed in the pineal gland and retina. At 2 months of age, NeuroD1 cKO retinas have a dramatic reduction in rod- and cone-driven electroretinograms and contain shortened and disorganized outer segments; by 4 months, NeuroD1 cKO retinas are devoid of photoreceptors. In contrast, the NeuroD1 cKO pineal gland appears histologically normal. Microarray analysis of 2-month-old NeuroD1 cKO retina and pineal gland identified a subset of genes that were affected 2-100-fold; in addition, a small group of genes exhibit altered differential night/day expression. Included in the down-regulated genes are Aipl1, which is necessary to prevent retinal degeneration, and Ankrd33, whose protein product is selectively expressed in the outer segments. These findings suggest that NeuroD1 may act through Aipl1 and other genes to maintain photoreceptor homeostasis.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/142317
Ochocinska, Margaret J.; Muñoz, Estela Maris; Veleri, Shobi; Weller, Joan L.; Coon, Steven L.; et al.; NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 123; 1; 10-2012; 44-59
0022-3042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/142317
identifier_str_mv Ochocinska, Margaret J.; Muñoz, Estela Maris; Veleri, Shobi; Weller, Joan L.; Coon, Steven L.; et al.; NeuroD1 is required for survival of photoreceptors but not pinealocytes: Results from targeted gene deletion studies; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 123; 1; 10-2012; 44-59
0022-3042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2012.07870.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2012.07870.x
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3441145/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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