Crx broadly modulates the pineal transcriptome

Autores
Rovsing, Louise; Clokie, Samuel; Bustos, Diego Martin; Rohde, Kristian; Coon, Steven L.; Litman, Thomas; Rath, Martin F.; Møller, Morten; Klein, David C.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx -/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.
Fil: Rovsing, Louise. Universidad de Copenhagen; Dinamarca. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Clokie, Samuel. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Rohde, Kristian. Universidad de Copenhagen; Dinamarca
Fil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Litman, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Rath, Martin F.. Universidad de Copenhagen; Dinamarca
Fil: Møller, Morten. Universidad de Copenhagen; Dinamarca
Fil: Klein, David C.. National Instituto of Child Health & Human Development; Estados Unidos
Materia
Crx
Gene Expression
Hoxc4
Microarray
Pineal Gland
Transcriptome Profiling
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/81345
Acceder
id CONICETDig_ca8790151db046c2dbad4434e3ed90e7
oai_identifier_str oai:ri.conicet.gov.ar:11336/81345
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Crx broadly modulates the pineal transcriptomeRovsing, LouiseClokie, SamuelBustos, Diego MartinRohde, KristianCoon, Steven L.Litman, ThomasRath, Martin F.Møller, MortenKlein, David C.CrxGene ExpressionHoxc4MicroarrayPineal GlandTranscriptome Profilinghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx -/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.Fil: Rovsing, Louise. Universidad de Copenhagen; Dinamarca. National Instituto of Child Health & Human Development; Estados UnidosFil: Clokie, Samuel. National Instituto of Child Health & Human Development; Estados UnidosFil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. National Instituto of Child Health & Human Development; Estados UnidosFil: Rohde, Kristian. Universidad de Copenhagen; DinamarcaFil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados UnidosFil: Litman, Thomas. Universidad de Copenhagen; DinamarcaFil: Rath, Martin F.. Universidad de Copenhagen; DinamarcaFil: Møller, Morten. Universidad de Copenhagen; DinamarcaFil: Klein, David C.. National Instituto of Child Health & Human Development; Estados UnidosWiley Blackwell Publishing, Inc2011-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/81345Rovsing, Louise; Clokie, Samuel; Bustos, Diego Martin; Rohde, Kristian; Coon, Steven L.; et al.; Crx broadly modulates the pineal transcriptome; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 119; 2; 10-2011; 262-2740022-3042CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2011.07405.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2011.07405.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:33:12Zoai:ri.conicet.gov.ar:11336/81345instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:33:13.248CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Crx broadly modulates the pineal transcriptome
title Crx broadly modulates the pineal transcriptome
spellingShingle Crx broadly modulates the pineal transcriptome
Rovsing, Louise
Crx
Gene Expression
Hoxc4
Microarray
Pineal Gland
Transcriptome Profiling
title_short Crx broadly modulates the pineal transcriptome
title_full Crx broadly modulates the pineal transcriptome
title_fullStr Crx broadly modulates the pineal transcriptome
title_full_unstemmed Crx broadly modulates the pineal transcriptome
title_sort Crx broadly modulates the pineal transcriptome
dc.creator.none.fl_str_mv Rovsing, Louise
Clokie, Samuel
Bustos, Diego Martin
Rohde, Kristian
Coon, Steven L.
Litman, Thomas
Rath, Martin F.
Møller, Morten
Klein, David C.
author Rovsing, Louise
author_facet Rovsing, Louise
Clokie, Samuel
Bustos, Diego Martin
Rohde, Kristian
Coon, Steven L.
Litman, Thomas
Rath, Martin F.
Møller, Morten
Klein, David C.
author_role author
author2 Clokie, Samuel
Bustos, Diego Martin
Rohde, Kristian
Coon, Steven L.
Litman, Thomas
Rath, Martin F.
Møller, Morten
Klein, David C.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Crx
Gene Expression
Hoxc4
Microarray
Pineal Gland
Transcriptome Profiling
topic Crx
Gene Expression
Hoxc4
Microarray
Pineal Gland
Transcriptome Profiling
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx -/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.
Fil: Rovsing, Louise. Universidad de Copenhagen; Dinamarca. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Clokie, Samuel. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Bustos, Diego Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Rohde, Kristian. Universidad de Copenhagen; Dinamarca
Fil: Coon, Steven L.. National Instituto of Child Health & Human Development; Estados Unidos
Fil: Litman, Thomas. Universidad de Copenhagen; Dinamarca
Fil: Rath, Martin F.. Universidad de Copenhagen; Dinamarca
Fil: Møller, Morten. Universidad de Copenhagen; Dinamarca
Fil: Klein, David C.. National Instituto of Child Health & Human Development; Estados Unidos
description Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx -/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.
publishDate 2011
dc.date.none.fl_str_mv 2011-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/81345
Rovsing, Louise; Clokie, Samuel; Bustos, Diego Martin; Rohde, Kristian; Coon, Steven L.; et al.; Crx broadly modulates the pineal transcriptome; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 119; 2; 10-2011; 262-274
0022-3042
CONICET Digital
CONICET
url http://hdl.handle.net/11336/81345
identifier_str_mv Rovsing, Louise; Clokie, Samuel; Bustos, Diego Martin; Rohde, Kristian; Coon, Steven L.; et al.; Crx broadly modulates the pineal transcriptome; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 119; 2; 10-2011; 262-274
0022-3042
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1471-4159.2011.07405.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2011.07405.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24909

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