Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland

Autores
Castro Zapata, Analia Elizabeth; Benitez, Sergio Gonzalo; Farías Altamirano, Luz Estefanía; Savastano, Luis E.; Patterson, Sean Ingram; Muñoz, Estela Maris
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Circadian rhythms govern many aspects of mammalian physiology.The daily pattern of melatonin synthesis and secretion is one of the classicexamples of circadian oscillations. It is mediated by a class of neuroendocrinecells known as pinealocytes which are not yet fully defined. An establishedmethod to evaluate functional and cytological characters is through theexpression of lineage-specific transcriptional regulators. NeuroD1 is a basichelix-loop-helix transcription factor involved in the specification andmaintenance of both endocrine and neuronal phenotypes. We have previouslydescribed developmental and adult regulation of NeuroD1 mRNA in therodent pineal gland. However, the transcript levels were not influenced by theelimination of sympathetic input, suggesting that any rhythmicity of NeuroD1might be found downstream of transcription. Here, we describe NeuroD1protein expression and cellular localization in the rat pineal gland duringdevelopment and the daily cycle. In embryonic and perinatal stages, proteinexpression follows the mRNA pattern and is predominantly nuclear.Thereafter, NeuroD1 is mostly found in pinealocyte nuclei in the early part ofthe night and in cytoplasm during the day, a rhythm maintained intoadulthood. Additionally, nocturnal nuclear NeuroD1 levels are reduced aftersympathetic disruption, an effect mimicked by the in vivo administration ofa- and b-adrenoceptor blockers. NeuroD1 phosphorylation at two sites, Ser274and Ser336, associates with nuclear localization in pinealocytes. These datasuggest that NeuroD1 influences pineal phenotype both during developmentand adulthood, in an autonomic and phosphorylation-dependent manner.
Fil: Castro Zapata, Analia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Farías Altamirano, Luz Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Savastano, Luis E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Patterson, Sean Ingram. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Materia
Neurod1
Nuclear-Cytoplasmic
Partitioning
Phosphorylation
Pineal Gland
Post-Translational
Serine Residues
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40520
Acceder
id CONICETDig_04850d5dce5583b787be55ce1d413969
oai_identifier_str oai:ri.conicet.gov.ar:11336/40520
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal glandCastro Zapata, Analia ElizabethBenitez, Sergio GonzaloFarías Altamirano, Luz EstefaníaSavastano, Luis E.Patterson, Sean IngramMuñoz, Estela MarisNeurod1Nuclear-CytoplasmicPartitioningPhosphorylationPineal GlandPost-TranslationalSerine Residueshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Circadian rhythms govern many aspects of mammalian physiology.The daily pattern of melatonin synthesis and secretion is one of the classicexamples of circadian oscillations. It is mediated by a class of neuroendocrinecells known as pinealocytes which are not yet fully defined. An establishedmethod to evaluate functional and cytological characters is through theexpression of lineage-specific transcriptional regulators. NeuroD1 is a basichelix-loop-helix transcription factor involved in the specification andmaintenance of both endocrine and neuronal phenotypes. We have previouslydescribed developmental and adult regulation of NeuroD1 mRNA in therodent pineal gland. However, the transcript levels were not influenced by theelimination of sympathetic input, suggesting that any rhythmicity of NeuroD1might be found downstream of transcription. Here, we describe NeuroD1protein expression and cellular localization in the rat pineal gland duringdevelopment and the daily cycle. In embryonic and perinatal stages, proteinexpression follows the mRNA pattern and is predominantly nuclear.Thereafter, NeuroD1 is mostly found in pinealocyte nuclei in the early part ofthe night and in cytoplasm during the day, a rhythm maintained intoadulthood. Additionally, nocturnal nuclear NeuroD1 levels are reduced aftersympathetic disruption, an effect mimicked by the in vivo administration ofa- and b-adrenoceptor blockers. NeuroD1 phosphorylation at two sites, Ser274and Ser336, associates with nuclear localization in pinealocytes. These datasuggest that NeuroD1 influences pineal phenotype both during developmentand adulthood, in an autonomic and phosphorylation-dependent manner.Fil: Castro Zapata, Analia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Farías Altamirano, Luz Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Savastano, Luis E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Patterson, Sean Ingram. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaFil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; ArgentinaWiley Blackwell Publishing, Inc2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40520Castro Zapata, Analia Elizabeth; Benitez, Sergio Gonzalo; Farías Altamirano, Luz Estefanía; Savastano, Luis E.; Patterson, Sean Ingram; et al.; Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 58; 4; 5-2015; 439-4510742-3098CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jpi.12228/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1111/jpi.12228info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:30Zoai:ri.conicet.gov.ar:11336/40520instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:31.021CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
title Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
spellingShingle Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
Castro Zapata, Analia Elizabeth
Neurod1
Nuclear-Cytoplasmic
Partitioning
Phosphorylation
Pineal Gland
Post-Translational
Serine Residues
title_short Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
title_full Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
title_fullStr Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
title_full_unstemmed Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
title_sort Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland
dc.creator.none.fl_str_mv Castro Zapata, Analia Elizabeth
Benitez, Sergio Gonzalo
Farías Altamirano, Luz Estefanía
Savastano, Luis E.
Patterson, Sean Ingram
Muñoz, Estela Maris
author Castro Zapata, Analia Elizabeth
author_facet Castro Zapata, Analia Elizabeth
Benitez, Sergio Gonzalo
Farías Altamirano, Luz Estefanía
Savastano, Luis E.
Patterson, Sean Ingram
Muñoz, Estela Maris
author_role author
author2 Benitez, Sergio Gonzalo
Farías Altamirano, Luz Estefanía
Savastano, Luis E.
Patterson, Sean Ingram
Muñoz, Estela Maris
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Neurod1
Nuclear-Cytoplasmic
Partitioning
Phosphorylation
Pineal Gland
Post-Translational
Serine Residues
topic Neurod1
Nuclear-Cytoplasmic
Partitioning
Phosphorylation
Pineal Gland
Post-Translational
Serine Residues
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Circadian rhythms govern many aspects of mammalian physiology.The daily pattern of melatonin synthesis and secretion is one of the classicexamples of circadian oscillations. It is mediated by a class of neuroendocrinecells known as pinealocytes which are not yet fully defined. An establishedmethod to evaluate functional and cytological characters is through theexpression of lineage-specific transcriptional regulators. NeuroD1 is a basichelix-loop-helix transcription factor involved in the specification andmaintenance of both endocrine and neuronal phenotypes. We have previouslydescribed developmental and adult regulation of NeuroD1 mRNA in therodent pineal gland. However, the transcript levels were not influenced by theelimination of sympathetic input, suggesting that any rhythmicity of NeuroD1might be found downstream of transcription. Here, we describe NeuroD1protein expression and cellular localization in the rat pineal gland duringdevelopment and the daily cycle. In embryonic and perinatal stages, proteinexpression follows the mRNA pattern and is predominantly nuclear.Thereafter, NeuroD1 is mostly found in pinealocyte nuclei in the early part ofthe night and in cytoplasm during the day, a rhythm maintained intoadulthood. Additionally, nocturnal nuclear NeuroD1 levels are reduced aftersympathetic disruption, an effect mimicked by the in vivo administration ofa- and b-adrenoceptor blockers. NeuroD1 phosphorylation at two sites, Ser274and Ser336, associates with nuclear localization in pinealocytes. These datasuggest that NeuroD1 influences pineal phenotype both during developmentand adulthood, in an autonomic and phosphorylation-dependent manner.
Fil: Castro Zapata, Analia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Benitez, Sergio Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Farías Altamirano, Luz Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Savastano, Luis E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Patterson, Sean Ingram. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
Fil: Muñoz, Estela Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
description Circadian rhythms govern many aspects of mammalian physiology.The daily pattern of melatonin synthesis and secretion is one of the classicexamples of circadian oscillations. It is mediated by a class of neuroendocrinecells known as pinealocytes which are not yet fully defined. An establishedmethod to evaluate functional and cytological characters is through theexpression of lineage-specific transcriptional regulators. NeuroD1 is a basichelix-loop-helix transcription factor involved in the specification andmaintenance of both endocrine and neuronal phenotypes. We have previouslydescribed developmental and adult regulation of NeuroD1 mRNA in therodent pineal gland. However, the transcript levels were not influenced by theelimination of sympathetic input, suggesting that any rhythmicity of NeuroD1might be found downstream of transcription. Here, we describe NeuroD1protein expression and cellular localization in the rat pineal gland duringdevelopment and the daily cycle. In embryonic and perinatal stages, proteinexpression follows the mRNA pattern and is predominantly nuclear.Thereafter, NeuroD1 is mostly found in pinealocyte nuclei in the early part ofthe night and in cytoplasm during the day, a rhythm maintained intoadulthood. Additionally, nocturnal nuclear NeuroD1 levels are reduced aftersympathetic disruption, an effect mimicked by the in vivo administration ofa- and b-adrenoceptor blockers. NeuroD1 phosphorylation at two sites, Ser274and Ser336, associates with nuclear localization in pinealocytes. These datasuggest that NeuroD1 influences pineal phenotype both during developmentand adulthood, in an autonomic and phosphorylation-dependent manner.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40520
Castro Zapata, Analia Elizabeth; Benitez, Sergio Gonzalo; Farías Altamirano, Luz Estefanía; Savastano, Luis E.; Patterson, Sean Ingram; et al.; Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 58; 4; 5-2015; 439-451
0742-3098
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40520
identifier_str_mv Castro Zapata, Analia Elizabeth; Benitez, Sergio Gonzalo; Farías Altamirano, Luz Estefanía; Savastano, Luis E.; Patterson, Sean Ingram; et al.; Expression and cellular localization of the transcription factor NeuroD1 in the developing and adult rat pineal gland; Wiley Blackwell Publishing, Inc; Journal of Pineal Research; 58; 4; 5-2015; 439-451
0742-3098
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jpi.12228/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1111/jpi.12228
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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