Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study

Autores
Busolini, Fabricio Iván; Rodríguez, Graciela Beatriz; Filippa, Veronica Palmira; Mohamed, Fabian Heber
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The presence of pigment has been demonstrated in different nervous structures such as those of retina, substantia nigra, and locus coeruleus. These pigments have also been described in the pineal gland of different mammal species. Histochemical and ultrastructural studies of the pineal gland of female viscacha (Lagostomus maximus maximus) were performed to analyze the presence of pigmented cells under natural conditions and to evaluate a probable relation between pigment content and glandular activity during pregnancy. The following techniques were applied: hematoxylin-eosin, phosphotungstic acid-hematoxylin, Masson-Fontana silver, DOPA histochemistry, Schmorl's reaction and toluidine blue. Estradiol and progesterone serum levels were determined by RIA. The ultrastructural features of the pineal pigment granules were also analyzed. Pigment granules were observed in a random distribution, but the pigmented cells were frequently found near blood vessels. The pineal pigment was histochemically identified as melanin. Differences in the amount of pigmented cells were found between pregnant and nonpregnant viscachas. The ultrastructural analysis revealed the presence of premelanosomes and melanosomes. Estradiol and progesterone levels vary during pregnancy. In conclusion, the changes in the amount of pigment content and hormone levels may indicate that the pineal gland of female viscacha is susceptible to endocrine variations during pregnancy.
Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Rodríguez, Graciela Beatriz. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
Lagostomus
Pineal gland
Ovarian hormones
Immunohistochemistry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/147207
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/147207
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural studyBusolini, Fabricio IvánRodríguez, Graciela BeatrizFilippa, Veronica PalmiraMohamed, Fabian HeberLagostomusPineal glandOvarian hormonesImmunohistochemistryhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The presence of pigment has been demonstrated in different nervous structures such as those of retina, substantia nigra, and locus coeruleus. These pigments have also been described in the pineal gland of different mammal species. Histochemical and ultrastructural studies of the pineal gland of female viscacha (Lagostomus maximus maximus) were performed to analyze the presence of pigmented cells under natural conditions and to evaluate a probable relation between pigment content and glandular activity during pregnancy. The following techniques were applied: hematoxylin-eosin, phosphotungstic acid-hematoxylin, Masson-Fontana silver, DOPA histochemistry, Schmorl's reaction and toluidine blue. Estradiol and progesterone serum levels were determined by RIA. The ultrastructural features of the pineal pigment granules were also analyzed. Pigment granules were observed in a random distribution, but the pigmented cells were frequently found near blood vessels. The pineal pigment was histochemically identified as melanin. Differences in the amount of pigmented cells were found between pregnant and nonpregnant viscachas. The ultrastructural analysis revealed the presence of premelanosomes and melanosomes. Estradiol and progesterone levels vary during pregnancy. In conclusion, the changes in the amount of pigment content and hormone levels may indicate that the pineal gland of female viscacha is susceptible to endocrine variations during pregnancy.Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Rodríguez, Graciela Beatriz. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaHindawi Limited2017-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/147207Busolini, Fabricio Iván; Rodríguez, Graciela Beatriz; Filippa, Veronica Palmira; Mohamed, Fabian Heber; Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study; Hindawi Limited; International Journal of Endocrinology; 2017; 7492960; 17-12-2017; 1-91687-8345CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ije/2017/7492960/info:eu-repo/semantics/altIdentifier/doi/10.1155/2017/7492960info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:50:39Zoai:ri.conicet.gov.ar:11336/147207instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:50:39.25CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
title Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
spellingShingle Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
Busolini, Fabricio Iván
Lagostomus
Pineal gland
Ovarian hormones
Immunohistochemistry
title_short Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
title_full Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
title_fullStr Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
title_full_unstemmed Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
title_sort Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study
dc.creator.none.fl_str_mv Busolini, Fabricio Iván
Rodríguez, Graciela Beatriz
Filippa, Veronica Palmira
Mohamed, Fabian Heber
author Busolini, Fabricio Iván
author_facet Busolini, Fabricio Iván
Rodríguez, Graciela Beatriz
Filippa, Veronica Palmira
Mohamed, Fabian Heber
author_role author
author2 Rodríguez, Graciela Beatriz
Filippa, Veronica Palmira
Mohamed, Fabian Heber
author2_role author
author
author
dc.subject.none.fl_str_mv Lagostomus
Pineal gland
Ovarian hormones
Immunohistochemistry
topic Lagostomus
Pineal gland
Ovarian hormones
Immunohistochemistry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The presence of pigment has been demonstrated in different nervous structures such as those of retina, substantia nigra, and locus coeruleus. These pigments have also been described in the pineal gland of different mammal species. Histochemical and ultrastructural studies of the pineal gland of female viscacha (Lagostomus maximus maximus) were performed to analyze the presence of pigmented cells under natural conditions and to evaluate a probable relation between pigment content and glandular activity during pregnancy. The following techniques were applied: hematoxylin-eosin, phosphotungstic acid-hematoxylin, Masson-Fontana silver, DOPA histochemistry, Schmorl's reaction and toluidine blue. Estradiol and progesterone serum levels were determined by RIA. The ultrastructural features of the pineal pigment granules were also analyzed. Pigment granules were observed in a random distribution, but the pigmented cells were frequently found near blood vessels. The pineal pigment was histochemically identified as melanin. Differences in the amount of pigmented cells were found between pregnant and nonpregnant viscachas. The ultrastructural analysis revealed the presence of premelanosomes and melanosomes. Estradiol and progesterone levels vary during pregnancy. In conclusion, the changes in the amount of pigment content and hormone levels may indicate that the pineal gland of female viscacha is susceptible to endocrine variations during pregnancy.
Fil: Busolini, Fabricio Iván. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Rodríguez, Graciela Beatriz. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Filippa, Veronica Palmira. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Mohamed, Fabian Heber. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description The presence of pigment has been demonstrated in different nervous structures such as those of retina, substantia nigra, and locus coeruleus. These pigments have also been described in the pineal gland of different mammal species. Histochemical and ultrastructural studies of the pineal gland of female viscacha (Lagostomus maximus maximus) were performed to analyze the presence of pigmented cells under natural conditions and to evaluate a probable relation between pigment content and glandular activity during pregnancy. The following techniques were applied: hematoxylin-eosin, phosphotungstic acid-hematoxylin, Masson-Fontana silver, DOPA histochemistry, Schmorl's reaction and toluidine blue. Estradiol and progesterone serum levels were determined by RIA. The ultrastructural features of the pineal pigment granules were also analyzed. Pigment granules were observed in a random distribution, but the pigmented cells were frequently found near blood vessels. The pineal pigment was histochemically identified as melanin. Differences in the amount of pigmented cells were found between pregnant and nonpregnant viscachas. The ultrastructural analysis revealed the presence of premelanosomes and melanosomes. Estradiol and progesterone levels vary during pregnancy. In conclusion, the changes in the amount of pigment content and hormone levels may indicate that the pineal gland of female viscacha is susceptible to endocrine variations during pregnancy.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/147207
Busolini, Fabricio Iván; Rodríguez, Graciela Beatriz; Filippa, Veronica Palmira; Mohamed, Fabian Heber; Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study; Hindawi Limited; International Journal of Endocrinology; 2017; 7492960; 17-12-2017; 1-9
1687-8345
CONICET Digital
CONICET
url http://hdl.handle.net/11336/147207
identifier_str_mv Busolini, Fabricio Iván; Rodríguez, Graciela Beatriz; Filippa, Veronica Palmira; Mohamed, Fabian Heber; Pigmented cells in the pineal gland of female viscacha (Lagostomus maximus maximus): A histochemical and ultrastructural study; Hindawi Limited; International Journal of Endocrinology; 2017; 7492960; 17-12-2017; 1-9
1687-8345
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/ije/2017/7492960/
info:eu-repo/semantics/altIdentifier/doi/10.1155/2017/7492960
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Limited
publisher.none.fl_str_mv Hindawi Limited
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613560640995328
score 13.070432

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