Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes

Autores
Nardy, Ana Flávia F.R.; Silva Filho, Joao Luiz da; Perez, Ana Rosa; Meis, Juliana de; Farias de Oliveira, Désio Aurélio; Penha, Luciana; Oliveira, Isadora de Araújo; Dias, Wagner B.; Todeschini, Adriane; Freire de Lima, Célio Geraldo; Bellio, Maria; Caruso Neves, Celso; Pinheiro, Ana Acácia; Takiya, Christina Maeda; Bottasso, Oscar Adelmo; Savino, Wilson; Morrot, Alexandre
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In experimental Trypanosoma cruzi infections, severe thymic atrophy leads to release of activated CD4+CD8+ double-positive (DP) T cells to the periphery. In humans, activated DP T cells are found in the blood in association with severe cardiac forms of human chronic Chagas disease. The mechanisms underlying the premature thymocyte release during the chagasic thymic atrophy remain elusive. We tested whether the migratory properties of intrathymic thymocytes are modulated by the parasite trans-sialidase (TS). We found that TS affected the dynamics of thymocytes undergoing intrathymic maturation, and these changes were accompanied by an increase in the number of recent DP thymic emigrants in the peripheral lymphoid organs. We demonstrated that increased percentages of blood DP T cell subsets were associated with augmented antibody titers against TS in chagasic patients with chronic cardiomyopathy. In vitro studies showed that TS was able to activate the MAPK pathway and actin filament mobilization in thymocytes. These effects were correlated with its ability to modulate the adhesion of thymocytes to thymic epithelial cells and their migration toward extracellular matrix. These findings point to effects of TS that could influence the escape of immature thymocytes in Chagas disease.
Fil: Nardy, Ana Flávia F.R.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva Filho, Joao Luiz da. Universidade Federal do Rio de Janeiro; Brasil
Fil: Perez, Ana Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Meis, Juliana de. Instituto Oswaldo Cruz; Brasil
Fil: Farias de Oliveira, Désio Aurélio. Instituto Oswaldo Cruz; Brasil
Fil: Penha, Luciana. Universidade Federal do Rio de Janeiro; Brasil
Fil: Oliveira, Isadora de Araújo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Dias, Wagner B.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Todeschini, Adriane. Universidade Federal do Rio de Janeiro; Brasil
Fil: Freire de Lima, Célio Geraldo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bellio, Maria. Universidade Federal do Rio de Janeiro; Brasil
Fil: Caruso Neves, Celso. Universidade Federal do Rio de Janeiro; Brasil
Fil: Pinheiro, Ana Acácia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Takiya, Christina Maeda. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bottasso, Oscar Adelmo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Savino, Wilson. Instituto Oswaldo Cruz; Brasil
Fil: Morrot, Alexandre. Universidade Federal do Rio de Janeiro; Brasil
Materia
Chagas
Thymus
Double-positive (DP) T cells
Transialidase
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/29186
Acceder
id CONICETDig_072ac02f02ae85d604b64c22c84f4211
oai_identifier_str oai:ri.conicet.gov.ar:11336/29186
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytesNardy, Ana Flávia F.R.Silva Filho, Joao Luiz daPerez, Ana RosaMeis, Juliana deFarias de Oliveira, Désio AurélioPenha, LucianaOliveira, Isadora de AraújoDias, Wagner B.Todeschini, AdrianeFreire de Lima, Célio GeraldoBellio, MariaCaruso Neves, CelsoPinheiro, Ana AcáciaTakiya, Christina MaedaBottasso, Oscar AdelmoSavino, WilsonMorrot, AlexandreChagasThymusDouble-positive (DP) T cellsTransialidasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In experimental Trypanosoma cruzi infections, severe thymic atrophy leads to release of activated CD4+CD8+ double-positive (DP) T cells to the periphery. In humans, activated DP T cells are found in the blood in association with severe cardiac forms of human chronic Chagas disease. The mechanisms underlying the premature thymocyte release during the chagasic thymic atrophy remain elusive. We tested whether the migratory properties of intrathymic thymocytes are modulated by the parasite trans-sialidase (TS). We found that TS affected the dynamics of thymocytes undergoing intrathymic maturation, and these changes were accompanied by an increase in the number of recent DP thymic emigrants in the peripheral lymphoid organs. We demonstrated that increased percentages of blood DP T cell subsets were associated with augmented antibody titers against TS in chagasic patients with chronic cardiomyopathy. In vitro studies showed that TS was able to activate the MAPK pathway and actin filament mobilization in thymocytes. These effects were correlated with its ability to modulate the adhesion of thymocytes to thymic epithelial cells and their migration toward extracellular matrix. These findings point to effects of TS that could influence the escape of immature thymocytes in Chagas disease.Fil: Nardy, Ana Flávia F.R.. Universidade Federal do Rio de Janeiro; BrasilFil: Silva Filho, Joao Luiz da. Universidade Federal do Rio de Janeiro; BrasilFil: Perez, Ana Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Meis, Juliana de. Instituto Oswaldo Cruz; BrasilFil: Farias de Oliveira, Désio Aurélio. Instituto Oswaldo Cruz; BrasilFil: Penha, Luciana. Universidade Federal do Rio de Janeiro; BrasilFil: Oliveira, Isadora de Araújo. Universidade Federal do Rio de Janeiro; BrasilFil: Dias, Wagner B.. Universidade Federal do Rio de Janeiro; BrasilFil: Todeschini, Adriane. Universidade Federal do Rio de Janeiro; BrasilFil: Freire de Lima, Célio Geraldo. Universidade Federal do Rio de Janeiro; BrasilFil: Bellio, Maria. Universidade Federal do Rio de Janeiro; BrasilFil: Caruso Neves, Celso. Universidade Federal do Rio de Janeiro; BrasilFil: Pinheiro, Ana Acácia. Universidade Federal do Rio de Janeiro; BrasilFil: Takiya, Christina Maeda. Universidade Federal do Rio de Janeiro; BrasilFil: Bottasso, Oscar Adelmo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Savino, Wilson. Instituto Oswaldo Cruz; BrasilFil: Morrot, Alexandre. Universidade Federal do Rio de Janeiro; BrasilElsevier Science2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/29186Nardy, Ana Flávia F.R.; Silva Filho, Joao Luiz da; Perez, Ana Rosa; Meis, Juliana de; Farias de Oliveira, Désio Aurélio; et al.; Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes; Elsevier Science; Microbes and Infection; 15; 5; 2-2013; 365-3741286-4579CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2013.02.003info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1286457913000324info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:07Zoai:ri.conicet.gov.ar:11336/29186instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:08.026CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
title Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
spellingShingle Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
Nardy, Ana Flávia F.R.
Chagas
Thymus
Double-positive (DP) T cells
Transialidase
title_short Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
title_full Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
title_fullStr Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
title_full_unstemmed Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
title_sort Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes
dc.creator.none.fl_str_mv Nardy, Ana Flávia F.R.
Silva Filho, Joao Luiz da
Perez, Ana Rosa
Meis, Juliana de
Farias de Oliveira, Désio Aurélio
Penha, Luciana
Oliveira, Isadora de Araújo
Dias, Wagner B.
Todeschini, Adriane
Freire de Lima, Célio Geraldo
Bellio, Maria
Caruso Neves, Celso
Pinheiro, Ana Acácia
Takiya, Christina Maeda
Bottasso, Oscar Adelmo
Savino, Wilson
Morrot, Alexandre
author Nardy, Ana Flávia F.R.
author_facet Nardy, Ana Flávia F.R.
Silva Filho, Joao Luiz da
Perez, Ana Rosa
Meis, Juliana de
Farias de Oliveira, Désio Aurélio
Penha, Luciana
Oliveira, Isadora de Araújo
Dias, Wagner B.
Todeschini, Adriane
Freire de Lima, Célio Geraldo
Bellio, Maria
Caruso Neves, Celso
Pinheiro, Ana Acácia
Takiya, Christina Maeda
Bottasso, Oscar Adelmo
Savino, Wilson
Morrot, Alexandre
author_role author
author2 Silva Filho, Joao Luiz da
Perez, Ana Rosa
Meis, Juliana de
Farias de Oliveira, Désio Aurélio
Penha, Luciana
Oliveira, Isadora de Araújo
Dias, Wagner B.
Todeschini, Adriane
Freire de Lima, Célio Geraldo
Bellio, Maria
Caruso Neves, Celso
Pinheiro, Ana Acácia
Takiya, Christina Maeda
Bottasso, Oscar Adelmo
Savino, Wilson
Morrot, Alexandre
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chagas
Thymus
Double-positive (DP) T cells
Transialidase
topic Chagas
Thymus
Double-positive (DP) T cells
Transialidase
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In experimental Trypanosoma cruzi infections, severe thymic atrophy leads to release of activated CD4+CD8+ double-positive (DP) T cells to the periphery. In humans, activated DP T cells are found in the blood in association with severe cardiac forms of human chronic Chagas disease. The mechanisms underlying the premature thymocyte release during the chagasic thymic atrophy remain elusive. We tested whether the migratory properties of intrathymic thymocytes are modulated by the parasite trans-sialidase (TS). We found that TS affected the dynamics of thymocytes undergoing intrathymic maturation, and these changes were accompanied by an increase in the number of recent DP thymic emigrants in the peripheral lymphoid organs. We demonstrated that increased percentages of blood DP T cell subsets were associated with augmented antibody titers against TS in chagasic patients with chronic cardiomyopathy. In vitro studies showed that TS was able to activate the MAPK pathway and actin filament mobilization in thymocytes. These effects were correlated with its ability to modulate the adhesion of thymocytes to thymic epithelial cells and their migration toward extracellular matrix. These findings point to effects of TS that could influence the escape of immature thymocytes in Chagas disease.
Fil: Nardy, Ana Flávia F.R.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Silva Filho, Joao Luiz da. Universidade Federal do Rio de Janeiro; Brasil
Fil: Perez, Ana Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Meis, Juliana de. Instituto Oswaldo Cruz; Brasil
Fil: Farias de Oliveira, Désio Aurélio. Instituto Oswaldo Cruz; Brasil
Fil: Penha, Luciana. Universidade Federal do Rio de Janeiro; Brasil
Fil: Oliveira, Isadora de Araújo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Dias, Wagner B.. Universidade Federal do Rio de Janeiro; Brasil
Fil: Todeschini, Adriane. Universidade Federal do Rio de Janeiro; Brasil
Fil: Freire de Lima, Célio Geraldo. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bellio, Maria. Universidade Federal do Rio de Janeiro; Brasil
Fil: Caruso Neves, Celso. Universidade Federal do Rio de Janeiro; Brasil
Fil: Pinheiro, Ana Acácia. Universidade Federal do Rio de Janeiro; Brasil
Fil: Takiya, Christina Maeda. Universidade Federal do Rio de Janeiro; Brasil
Fil: Bottasso, Oscar Adelmo. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Savino, Wilson. Instituto Oswaldo Cruz; Brasil
Fil: Morrot, Alexandre. Universidade Federal do Rio de Janeiro; Brasil
description In experimental Trypanosoma cruzi infections, severe thymic atrophy leads to release of activated CD4+CD8+ double-positive (DP) T cells to the periphery. In humans, activated DP T cells are found in the blood in association with severe cardiac forms of human chronic Chagas disease. The mechanisms underlying the premature thymocyte release during the chagasic thymic atrophy remain elusive. We tested whether the migratory properties of intrathymic thymocytes are modulated by the parasite trans-sialidase (TS). We found that TS affected the dynamics of thymocytes undergoing intrathymic maturation, and these changes were accompanied by an increase in the number of recent DP thymic emigrants in the peripheral lymphoid organs. We demonstrated that increased percentages of blood DP T cell subsets were associated with augmented antibody titers against TS in chagasic patients with chronic cardiomyopathy. In vitro studies showed that TS was able to activate the MAPK pathway and actin filament mobilization in thymocytes. These effects were correlated with its ability to modulate the adhesion of thymocytes to thymic epithelial cells and their migration toward extracellular matrix. These findings point to effects of TS that could influence the escape of immature thymocytes in Chagas disease.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/29186
Nardy, Ana Flávia F.R.; Silva Filho, Joao Luiz da; Perez, Ana Rosa; Meis, Juliana de; Farias de Oliveira, Désio Aurélio; et al.; Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes; Elsevier Science; Microbes and Infection; 15; 5; 2-2013; 365-374
1286-4579
CONICET Digital
CONICET
url http://hdl.handle.net/11336/29186
identifier_str_mv Nardy, Ana Flávia F.R.; Silva Filho, Joao Luiz da; Perez, Ana Rosa; Meis, Juliana de; Farias de Oliveira, Désio Aurélio; et al.; Trans-sialidase from Trypanosoma cruzi enhances the adhesion properties and fibronectin-driven migration of thymocytes; Elsevier Science; Microbes and Infection; 15; 5; 2-2013; 365-374
1286-4579
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micinf.2013.02.003
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1286457913000324
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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