TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells

Autores
Perez, Ana Rosa; Berbert, Luiz Ricardo; Lepletier, Ailin; Revelli, Silvia Susana; Bottasso, Oscar Adelmo; Silva Barbosa, Suse Dayse; Savino, Wilson
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Migratory activity of thymocytes and mature T cell activity seem to be finely tuned by cytokines, chemokines and extracellular matrix components. Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental infection with Trypanosoma cruzi, the causative agent of Chagas disease. TNF-alpha production is enhanced during infection and appears to be crucial in the response to the parasite. However, it also seems to play a role in disease pathology, since it is involved in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-alpha in the migratory activity of thymocytes in T. cruzi acutely-infected mice. We found increased expression and deposition of TNF-alpha in the thymus of infected animals compared to controls. This was accompanied by increased co-localization of this cytokine with fibronectin, a cell migration-related extracellular matrix molecule, whose contents in the thymus of infected mice is also augmented. In vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-alpha. The Increase in absolute and relative numbers of immature CD4+CD8+ T cells in secondary lymphoid organs were even more clear-cut when TNF-alpha was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-alpha was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4+CD8+ and CD4-CD8- cells. Our findings suggest that in T. cruzi acute infection, when TNF-alpha is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-alpha is a further player in the process.
Fil: Perez, Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Berbert, Luiz Ricardo. Instituto Oswaldo Cruz; Brasil
Fil: Lepletier, Ailin. Instituto Oswaldo Cruz; Brasil
Fil: Revelli, Silvia Susana. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Silva Barbosa, Suse Dayse. Instituto Oswaldo Cruz; Brasil
Fil: Savino, Wilson. Instituto Oswaldo Cruz; Brasil
Materia
Thymocyte Migration
TNF-alpha
Thymus
Chagas Disease
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/268990
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/268990
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature CellsPerez, Ana RosaBerbert, Luiz RicardoLepletier, AilinRevelli, Silvia SusanaBottasso, Oscar AdelmoSilva Barbosa, Suse DayseSavino, WilsonThymocyte MigrationTNF-alphaThymusChagas Diseasehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Migratory activity of thymocytes and mature T cell activity seem to be finely tuned by cytokines, chemokines and extracellular matrix components. Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental infection with Trypanosoma cruzi, the causative agent of Chagas disease. TNF-alpha production is enhanced during infection and appears to be crucial in the response to the parasite. However, it also seems to play a role in disease pathology, since it is involved in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-alpha in the migratory activity of thymocytes in T. cruzi acutely-infected mice. We found increased expression and deposition of TNF-alpha in the thymus of infected animals compared to controls. This was accompanied by increased co-localization of this cytokine with fibronectin, a cell migration-related extracellular matrix molecule, whose contents in the thymus of infected mice is also augmented. In vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-alpha. The Increase in absolute and relative numbers of immature CD4+CD8+ T cells in secondary lymphoid organs were even more clear-cut when TNF-alpha was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-alpha was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4+CD8+ and CD4-CD8- cells. Our findings suggest that in T. cruzi acute infection, when TNF-alpha is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-alpha is a further player in the process.Fil: Perez, Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; ArgentinaFil: Berbert, Luiz Ricardo. Instituto Oswaldo Cruz; BrasilFil: Lepletier, Ailin. Instituto Oswaldo Cruz; BrasilFil: Revelli, Silvia Susana. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; ArgentinaFil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; ArgentinaFil: Silva Barbosa, Suse Dayse. Instituto Oswaldo Cruz; BrasilFil: Savino, Wilson. Instituto Oswaldo Cruz; BrasilPublic Library of Science2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/268990Perez, Ana Rosa; Berbert, Luiz Ricardo; Lepletier, Ailin; Revelli, Silvia Susana; Bottasso, Oscar Adelmo; et al.; TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells; Public Library of Science; Plos One; 7; 3; 2-2012; 1-101932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034360info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0034360info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:32:41Zoai:ri.conicet.gov.ar:11336/268990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:32:41.781CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
title TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
spellingShingle TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
Perez, Ana Rosa
Thymocyte Migration
TNF-alpha
Thymus
Chagas Disease
title_short TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
title_full TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
title_fullStr TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
title_full_unstemmed TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
title_sort TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells
dc.creator.none.fl_str_mv Perez, Ana Rosa
Berbert, Luiz Ricardo
Lepletier, Ailin
Revelli, Silvia Susana
Bottasso, Oscar Adelmo
Silva Barbosa, Suse Dayse
Savino, Wilson
author Perez, Ana Rosa
author_facet Perez, Ana Rosa
Berbert, Luiz Ricardo
Lepletier, Ailin
Revelli, Silvia Susana
Bottasso, Oscar Adelmo
Silva Barbosa, Suse Dayse
Savino, Wilson
author_role author
author2 Berbert, Luiz Ricardo
Lepletier, Ailin
Revelli, Silvia Susana
Bottasso, Oscar Adelmo
Silva Barbosa, Suse Dayse
Savino, Wilson
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Thymocyte Migration
TNF-alpha
Thymus
Chagas Disease
topic Thymocyte Migration
TNF-alpha
Thymus
Chagas Disease
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Migratory activity of thymocytes and mature T cell activity seem to be finely tuned by cytokines, chemokines and extracellular matrix components. Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental infection with Trypanosoma cruzi, the causative agent of Chagas disease. TNF-alpha production is enhanced during infection and appears to be crucial in the response to the parasite. However, it also seems to play a role in disease pathology, since it is involved in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-alpha in the migratory activity of thymocytes in T. cruzi acutely-infected mice. We found increased expression and deposition of TNF-alpha in the thymus of infected animals compared to controls. This was accompanied by increased co-localization of this cytokine with fibronectin, a cell migration-related extracellular matrix molecule, whose contents in the thymus of infected mice is also augmented. In vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-alpha. The Increase in absolute and relative numbers of immature CD4+CD8+ T cells in secondary lymphoid organs were even more clear-cut when TNF-alpha was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-alpha was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4+CD8+ and CD4-CD8- cells. Our findings suggest that in T. cruzi acute infection, when TNF-alpha is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-alpha is a further player in the process.
Fil: Perez, Ana Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Berbert, Luiz Ricardo. Instituto Oswaldo Cruz; Brasil
Fil: Lepletier, Ailin. Instituto Oswaldo Cruz; Brasil
Fil: Revelli, Silvia Susana. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Bottasso, Oscar Adelmo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Instituto de Inmunología; Argentina
Fil: Silva Barbosa, Suse Dayse. Instituto Oswaldo Cruz; Brasil
Fil: Savino, Wilson. Instituto Oswaldo Cruz; Brasil
description Migratory activity of thymocytes and mature T cell activity seem to be finely tuned by cytokines, chemokines and extracellular matrix components. Previous studies revealed a significant production of inflammatory cytokines together with severe thymic atrophy and thymocyte migratory disturbances during experimental infection with Trypanosoma cruzi, the causative agent of Chagas disease. TNF-alpha production is enhanced during infection and appears to be crucial in the response to the parasite. However, it also seems to play a role in disease pathology, since it is involved in the arrival of T cells to effector sites, including the myocardium. Herein, we analyzed the role of TNF-alpha in the migratory activity of thymocytes in T. cruzi acutely-infected mice. We found increased expression and deposition of TNF-alpha in the thymus of infected animals compared to controls. This was accompanied by increased co-localization of this cytokine with fibronectin, a cell migration-related extracellular matrix molecule, whose contents in the thymus of infected mice is also augmented. In vivo studies showed an enhanced export of thymocytes in T. cruzi-infected mice, as ascertained by intrathymic injection of FITC alone or in combination with TNF-alpha. The Increase in absolute and relative numbers of immature CD4+CD8+ T cells in secondary lymphoid organs were even more clear-cut when TNF-alpha was co-injected with FITC. Ex-vivo transmigration assays also revealed higher number of migrating cells when TNF-alpha was added onto fibronectin lattices, with higher input of all thymocyte subsets, including immature CD4+CD8+ and CD4-CD8- cells. Our findings suggest that in T. cruzi acute infection, when TNF-alpha is complexed with fibronectin, it favours the altered migration of thymocytes, promoting the release of mature and immature T cells to different compartments of the immune system. Conceptually, this work reinforces the notion that thymocyte migration is a multivectorial biological event in health and disease, and that TNF-alpha is a further player in the process.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/268990
Perez, Ana Rosa; Berbert, Luiz Ricardo; Lepletier, Ailin; Revelli, Silvia Susana; Bottasso, Oscar Adelmo; et al.; TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells; Public Library of Science; Plos One; 7; 3; 2-2012; 1-10
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/268990
identifier_str_mv Perez, Ana Rosa; Berbert, Luiz Ricardo; Lepletier, Ailin; Revelli, Silvia Susana; Bottasso, Oscar Adelmo; et al.; TNF-α Is Involved in the Abnormal Thymocyte Migration during Experimental Trypanosoma cruzi Infection and Favors the Export of Immature Cells; Public Library of Science; Plos One; 7; 3; 2-2012; 1-10
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0034360
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0034360
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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