Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units

Autores
Burgos, Juan Miguel; Risso, Marikena Guadalupe; Brenière, Simone Frédérique; Barnabé, Christian; Campetella, Oscar Eduardo; Leguizamon, Maria Susana
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks. Genes encoding TS-related proteins are included in a large family divided in several groups but only one of them contains TS genes. Two closely related genes differing in a T/C transition encode the enzymatically active TS (aTS) and a lectin-like TS (iTS). We quantified the aTS/iTS genes from TcII and TcVI aggressive and TcI low virulent strains and found variable aTS number (1–32) per haploid genome. In spite of being low TS enzyme-expressers, TcI strains carry 28–32 aTS gene copies. The intriguing absence of iTS genes in TcI strains together with the presence of aTS/iTS in TcII and TcVI strains (virulent) were observed. Moreover, after sequencing aTS/iTS from 38 isolates collected along the Americas encompassing all DTUs, the persistent absence of the iTS gene in TcI, TcIII and TcIV was found. In addition, the sequence clustering together with T/C transition analysis correlated to DTUs of T. cruzi. The consistence of TS results with both evolutionary genome models proposed for T. cruzi, namely the “Two Hybridization” and the “Three Ancestor” was discussed and reviewed to fit present findings. Parasite stocks to attempt genetic KO or to assay the involvement of iTS in parasite biology and virulence are finally available.
Fil: Burgos, Juan Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Risso, Marikena Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Brenière, Simone Frédérique. Institut de Recherche pour le Développement. Unité de Recherche Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle; Francia
Fil: Barnabé, Christian. Institut de Recherche pour le Développement. Unité de Recherche Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle; Francia
Fil: Campetella, Oscar Eduardo. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leguizamon, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones En Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones En Microbiología y Parasitología Médica; Argentina
Materia
TRYPANOSOMA CRUZI
TRANSIALIDASE
DISCRETE TYPING UNIT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16829
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing unitsBurgos, Juan MiguelRisso, Marikena GuadalupeBrenière, Simone FrédériqueBarnabé, ChristianCampetella, Oscar EduardoLeguizamon, Maria SusanaTRYPANOSOMA CRUZITRANSIALIDASEDISCRETE TYPING UNIThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks. Genes encoding TS-related proteins are included in a large family divided in several groups but only one of them contains TS genes. Two closely related genes differing in a T/C transition encode the enzymatically active TS (aTS) and a lectin-like TS (iTS). We quantified the aTS/iTS genes from TcII and TcVI aggressive and TcI low virulent strains and found variable aTS number (1–32) per haploid genome. In spite of being low TS enzyme-expressers, TcI strains carry 28–32 aTS gene copies. The intriguing absence of iTS genes in TcI strains together with the presence of aTS/iTS in TcII and TcVI strains (virulent) were observed. Moreover, after sequencing aTS/iTS from 38 isolates collected along the Americas encompassing all DTUs, the persistent absence of the iTS gene in TcI, TcIII and TcIV was found. In addition, the sequence clustering together with T/C transition analysis correlated to DTUs of T. cruzi. The consistence of TS results with both evolutionary genome models proposed for T. cruzi, namely the “Two Hybridization” and the “Three Ancestor” was discussed and reviewed to fit present findings. Parasite stocks to attempt genetic KO or to assay the involvement of iTS in parasite biology and virulence are finally available.Fil: Burgos, Juan Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Risso, Marikena Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Brenière, Simone Frédérique. Institut de Recherche pour le Développement. Unité de Recherche Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle; FranciaFil: Barnabé, Christian. Institut de Recherche pour le Développement. Unité de Recherche Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle; FranciaFil: Campetella, Oscar Eduardo. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leguizamon, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones En Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones En Microbiología y Parasitología Médica; ArgentinaPublic Library Of Science2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16829Burgos, Juan Miguel; Risso, Marikena Guadalupe; Brenière, Simone Frédérique; Barnabé, Christian; Campetella, Oscar Eduardo; et al.; Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units; Public Library Of Science; Plos One; 8; 3; 3-2013; 1-8; e589671932-6203enginfo:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0058967info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0058967info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594200/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:05:26Zoai:ri.conicet.gov.ar:11336/16829instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:05:27.243CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
title Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
spellingShingle Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
Burgos, Juan Miguel
TRYPANOSOMA CRUZI
TRANSIALIDASE
DISCRETE TYPING UNIT
title_short Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
title_full Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
title_fullStr Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
title_full_unstemmed Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
title_sort Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units
dc.creator.none.fl_str_mv Burgos, Juan Miguel
Risso, Marikena Guadalupe
Brenière, Simone Frédérique
Barnabé, Christian
Campetella, Oscar Eduardo
Leguizamon, Maria Susana
author Burgos, Juan Miguel
author_facet Burgos, Juan Miguel
Risso, Marikena Guadalupe
Brenière, Simone Frédérique
Barnabé, Christian
Campetella, Oscar Eduardo
Leguizamon, Maria Susana
author_role author
author2 Risso, Marikena Guadalupe
Brenière, Simone Frédérique
Barnabé, Christian
Campetella, Oscar Eduardo
Leguizamon, Maria Susana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv TRYPANOSOMA CRUZI
TRANSIALIDASE
DISCRETE TYPING UNIT
topic TRYPANOSOMA CRUZI
TRANSIALIDASE
DISCRETE TYPING UNIT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks. Genes encoding TS-related proteins are included in a large family divided in several groups but only one of them contains TS genes. Two closely related genes differing in a T/C transition encode the enzymatically active TS (aTS) and a lectin-like TS (iTS). We quantified the aTS/iTS genes from TcII and TcVI aggressive and TcI low virulent strains and found variable aTS number (1–32) per haploid genome. In spite of being low TS enzyme-expressers, TcI strains carry 28–32 aTS gene copies. The intriguing absence of iTS genes in TcI strains together with the presence of aTS/iTS in TcII and TcVI strains (virulent) were observed. Moreover, after sequencing aTS/iTS from 38 isolates collected along the Americas encompassing all DTUs, the persistent absence of the iTS gene in TcI, TcIII and TcIV was found. In addition, the sequence clustering together with T/C transition analysis correlated to DTUs of T. cruzi. The consistence of TS results with both evolutionary genome models proposed for T. cruzi, namely the “Two Hybridization” and the “Three Ancestor” was discussed and reviewed to fit present findings. Parasite stocks to attempt genetic KO or to assay the involvement of iTS in parasite biology and virulence are finally available.
Fil: Burgos, Juan Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Risso, Marikena Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Brenière, Simone Frédérique. Institut de Recherche pour le Développement. Unité de Recherche Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle; Francia
Fil: Barnabé, Christian. Institut de Recherche pour le Développement. Unité de Recherche Maladies Infectieuses et Vecteurs : Ecologie, Génétique, Evolution et Contrôle; Francia
Fil: Campetella, Oscar Eduardo. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leguizamon, Maria Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones En Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones En Microbiología y Parasitología Médica; Argentina
description Trypanosoma cruzi the agent of Chagas disease is a monophyletic but heterogeneous group conformed by several Discrete Typing Units (DTUs) named TcI to TcVI characterized by genetic markers. The trans-sialidase (TS) is a virulence factor involved in cell invasion and pathogenesis that is differentially expressed in aggressive and less virulent parasite stocks. Genes encoding TS-related proteins are included in a large family divided in several groups but only one of them contains TS genes. Two closely related genes differing in a T/C transition encode the enzymatically active TS (aTS) and a lectin-like TS (iTS). We quantified the aTS/iTS genes from TcII and TcVI aggressive and TcI low virulent strains and found variable aTS number (1–32) per haploid genome. In spite of being low TS enzyme-expressers, TcI strains carry 28–32 aTS gene copies. The intriguing absence of iTS genes in TcI strains together with the presence of aTS/iTS in TcII and TcVI strains (virulent) were observed. Moreover, after sequencing aTS/iTS from 38 isolates collected along the Americas encompassing all DTUs, the persistent absence of the iTS gene in TcI, TcIII and TcIV was found. In addition, the sequence clustering together with T/C transition analysis correlated to DTUs of T. cruzi. The consistence of TS results with both evolutionary genome models proposed for T. cruzi, namely the “Two Hybridization” and the “Three Ancestor” was discussed and reviewed to fit present findings. Parasite stocks to attempt genetic KO or to assay the involvement of iTS in parasite biology and virulence are finally available.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16829
Burgos, Juan Miguel; Risso, Marikena Guadalupe; Brenière, Simone Frédérique; Barnabé, Christian; Campetella, Oscar Eduardo; et al.; Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units; Public Library Of Science; Plos One; 8; 3; 3-2013; 1-8; e58967
1932-6203
url http://hdl.handle.net/11336/16829
identifier_str_mv Burgos, Juan Miguel; Risso, Marikena Guadalupe; Brenière, Simone Frédérique; Barnabé, Christian; Campetella, Oscar Eduardo; et al.; Differential distribution of genes encoding the virulence factor trans-sialidase along Trypanosoma cruzi discrete typing units; Public Library Of Science; Plos One; 8; 3; 3-2013; 1-8; e58967
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0058967
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0058967
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3594200/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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