Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection

Autores
Silva Monteiro, Elizangela; Reis Lorenzato, Luciana; Kenji Nihei, Oscar; Junqueira, Mara; Rabinovich, Gabriel Adrián; Kaiyuan Hsu, Daniel; Liu, Fu Tong; Savino, Wilson; Chammas, Roger; Serra Villaverde, Dea Maria
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During acute infection with Trypanosoma cruzi, the causative agent of Chagas´ disease, the thymus undergoes intense atrophy followed by a premature escape of CD4+CD8+ immature cortical thymocytes. Here we report a pivotal role for the endogenous lectin galectin-3 in accelerating death of thymocytes and migration of these cells away from the thymus after T. cruzi infection. We observed a pronounced increase in galectin-3 expression that paralleled the extensive depletion of CD4+CD8+ immature thymocytes after infection. In vitro, recombinant galectin-3 induced increased levels of death in cortical immature thymocytes. Consistent with the role of galectin-3 in promoting cell death, thymuses from gal-3-/- mice did not show cortical thymocyte depletion after parasite infection in vivo. In addition, galectin-3 accelerated laminin-driven CD4+CD8+ thymocyte migration in vitro and in vivo induced exportation of CD4+CD8+ cells from the thymus to the peripheral compartment. Our findings provide evidence of a novel role for galectin-3 in the regulation of thymus physiology and identify a potential mechanism based on protein-glycan interactions in thymic atrophy associated with acute T. cruzi infection
Fil: Silva Monteiro, Elizangela. Instituto Oswaldo Cruz; Brasil
Fil: Reis Lorenzato, Luciana. Instituto Oswaldo Cruz; Brasil
Fil: Kenji Nihei, Oscar. Instituto Oswaldo Cruz; Brasil
Fil: Junqueira, Mara. Universidade de Sao Paulo; Brasil
Fil: Rabinovich, Gabriel Adrián. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Kaiyuan Hsu, Daniel. University of California; Estados Unidos
Fil: Liu, Fu Tong. University of California; Estados Unidos
Fil: Savino, Wilson. Instituto Oswaldo Cruz; Brasil
Fil: Chammas, Roger. Universidade de Sao Paulo; Brasil
Fil: Serra Villaverde, Dea Maria. Instituto Oswaldo Cruz; Brasil
Materia
Cell Death
Chagas Disease
Galectin-3
Thymus Gland
Trypanosoma Cruzi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27503
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infectionSilva Monteiro, ElizangelaReis Lorenzato, LucianaKenji Nihei, OscarJunqueira, MaraRabinovich, Gabriel AdriánKaiyuan Hsu, DanielLiu, Fu TongSavino, WilsonChammas, RogerSerra Villaverde, Dea MariaCell DeathChagas DiseaseGalectin-3Thymus GlandTrypanosoma Cruzihttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3During acute infection with Trypanosoma cruzi, the causative agent of Chagas´ disease, the thymus undergoes intense atrophy followed by a premature escape of CD4+CD8+ immature cortical thymocytes. Here we report a pivotal role for the endogenous lectin galectin-3 in accelerating death of thymocytes and migration of these cells away from the thymus after T. cruzi infection. We observed a pronounced increase in galectin-3 expression that paralleled the extensive depletion of CD4+CD8+ immature thymocytes after infection. In vitro, recombinant galectin-3 induced increased levels of death in cortical immature thymocytes. Consistent with the role of galectin-3 in promoting cell death, thymuses from gal-3-/- mice did not show cortical thymocyte depletion after parasite infection in vivo. In addition, galectin-3 accelerated laminin-driven CD4+CD8+ thymocyte migration in vitro and in vivo induced exportation of CD4+CD8+ cells from the thymus to the peripheral compartment. Our findings provide evidence of a novel role for galectin-3 in the regulation of thymus physiology and identify a potential mechanism based on protein-glycan interactions in thymic atrophy associated with acute T. cruzi infectionFil: Silva Monteiro, Elizangela. Instituto Oswaldo Cruz; BrasilFil: Reis Lorenzato, Luciana. Instituto Oswaldo Cruz; BrasilFil: Kenji Nihei, Oscar. Instituto Oswaldo Cruz; BrasilFil: Junqueira, Mara. Universidade de Sao Paulo; BrasilFil: Rabinovich, Gabriel Adrián. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; ArgentinaFil: Kaiyuan Hsu, Daniel. University of California; Estados UnidosFil: Liu, Fu Tong. University of California; Estados UnidosFil: Savino, Wilson. Instituto Oswaldo Cruz; BrasilFil: Chammas, Roger. Universidade de Sao Paulo; BrasilFil: Serra Villaverde, Dea Maria. Instituto Oswaldo Cruz; BrasilAmer Soc Investigative Pathology, Inc2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27503Silva Monteiro, Elizangela; Reis Lorenzato, Luciana; Kenji Nihei, Oscar; Junqueira, Mara; Rabinovich, Gabriel Adrián; et al.; Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 170; 2; 2007; 546-5560002-94401525-2191CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ajp.amjpathol.org/article/S0002-9440(10)60878-4/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.2353/ajpath.2007.060389info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851869/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:28Zoai:ri.conicet.gov.ar:11336/27503instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:28.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
title Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
spellingShingle Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
Silva Monteiro, Elizangela
Cell Death
Chagas Disease
Galectin-3
Thymus Gland
Trypanosoma Cruzi
title_short Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
title_full Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
title_fullStr Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
title_full_unstemmed Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
title_sort Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection
dc.creator.none.fl_str_mv Silva Monteiro, Elizangela
Reis Lorenzato, Luciana
Kenji Nihei, Oscar
Junqueira, Mara
Rabinovich, Gabriel Adrián
Kaiyuan Hsu, Daniel
Liu, Fu Tong
Savino, Wilson
Chammas, Roger
Serra Villaverde, Dea Maria
author Silva Monteiro, Elizangela
author_facet Silva Monteiro, Elizangela
Reis Lorenzato, Luciana
Kenji Nihei, Oscar
Junqueira, Mara
Rabinovich, Gabriel Adrián
Kaiyuan Hsu, Daniel
Liu, Fu Tong
Savino, Wilson
Chammas, Roger
Serra Villaverde, Dea Maria
author_role author
author2 Reis Lorenzato, Luciana
Kenji Nihei, Oscar
Junqueira, Mara
Rabinovich, Gabriel Adrián
Kaiyuan Hsu, Daniel
Liu, Fu Tong
Savino, Wilson
Chammas, Roger
Serra Villaverde, Dea Maria
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cell Death
Chagas Disease
Galectin-3
Thymus Gland
Trypanosoma Cruzi
topic Cell Death
Chagas Disease
Galectin-3
Thymus Gland
Trypanosoma Cruzi
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv During acute infection with Trypanosoma cruzi, the causative agent of Chagas´ disease, the thymus undergoes intense atrophy followed by a premature escape of CD4+CD8+ immature cortical thymocytes. Here we report a pivotal role for the endogenous lectin galectin-3 in accelerating death of thymocytes and migration of these cells away from the thymus after T. cruzi infection. We observed a pronounced increase in galectin-3 expression that paralleled the extensive depletion of CD4+CD8+ immature thymocytes after infection. In vitro, recombinant galectin-3 induced increased levels of death in cortical immature thymocytes. Consistent with the role of galectin-3 in promoting cell death, thymuses from gal-3-/- mice did not show cortical thymocyte depletion after parasite infection in vivo. In addition, galectin-3 accelerated laminin-driven CD4+CD8+ thymocyte migration in vitro and in vivo induced exportation of CD4+CD8+ cells from the thymus to the peripheral compartment. Our findings provide evidence of a novel role for galectin-3 in the regulation of thymus physiology and identify a potential mechanism based on protein-glycan interactions in thymic atrophy associated with acute T. cruzi infection
Fil: Silva Monteiro, Elizangela. Instituto Oswaldo Cruz; Brasil
Fil: Reis Lorenzato, Luciana. Instituto Oswaldo Cruz; Brasil
Fil: Kenji Nihei, Oscar. Instituto Oswaldo Cruz; Brasil
Fil: Junqueira, Mara. Universidade de Sao Paulo; Brasil
Fil: Rabinovich, Gabriel Adrián. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
Fil: Kaiyuan Hsu, Daniel. University of California; Estados Unidos
Fil: Liu, Fu Tong. University of California; Estados Unidos
Fil: Savino, Wilson. Instituto Oswaldo Cruz; Brasil
Fil: Chammas, Roger. Universidade de Sao Paulo; Brasil
Fil: Serra Villaverde, Dea Maria. Instituto Oswaldo Cruz; Brasil
description During acute infection with Trypanosoma cruzi, the causative agent of Chagas´ disease, the thymus undergoes intense atrophy followed by a premature escape of CD4+CD8+ immature cortical thymocytes. Here we report a pivotal role for the endogenous lectin galectin-3 in accelerating death of thymocytes and migration of these cells away from the thymus after T. cruzi infection. We observed a pronounced increase in galectin-3 expression that paralleled the extensive depletion of CD4+CD8+ immature thymocytes after infection. In vitro, recombinant galectin-3 induced increased levels of death in cortical immature thymocytes. Consistent with the role of galectin-3 in promoting cell death, thymuses from gal-3-/- mice did not show cortical thymocyte depletion after parasite infection in vivo. In addition, galectin-3 accelerated laminin-driven CD4+CD8+ thymocyte migration in vitro and in vivo induced exportation of CD4+CD8+ cells from the thymus to the peripheral compartment. Our findings provide evidence of a novel role for galectin-3 in the regulation of thymus physiology and identify a potential mechanism based on protein-glycan interactions in thymic atrophy associated with acute T. cruzi infection
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27503
Silva Monteiro, Elizangela; Reis Lorenzato, Luciana; Kenji Nihei, Oscar; Junqueira, Mara; Rabinovich, Gabriel Adrián; et al.; Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 170; 2; 2007; 546-556
0002-9440
1525-2191
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27503
identifier_str_mv Silva Monteiro, Elizangela; Reis Lorenzato, Luciana; Kenji Nihei, Oscar; Junqueira, Mara; Rabinovich, Gabriel Adrián; et al.; Altered expression of galectin-3 induces cortical thymocyte depletion and premature exit of immature thymocytes during Trypanosoma cruzi infection; Amer Soc Investigative Pathology, Inc; American Journal Of Pathology; 170; 2; 2007; 546-556
0002-9440
1525-2191
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ajp.amjpathol.org/article/S0002-9440(10)60878-4/abstract
info:eu-repo/semantics/altIdentifier/doi/10.2353/ajpath.2007.060389
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1851869/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Amer Soc Investigative Pathology, Inc
publisher.none.fl_str_mv Amer Soc Investigative Pathology, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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