Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR

Autores
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Favier, Gabriela Isabel; Di Genaro, Maria Silvia; Escudero, María Esther
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The prevalence of Yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (A), enrichment in modified Rappaport broth at 25 C (B), concentration by immunomagnetic separation (C) and yadA nested PCR (D). Furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (PFGE). A total of 238 samples were collected at retail level in the city of San Luis, Argentina, during the period 2007e2008. The highest Yersinia prevalence in meat products was observed by method D (92 positive samples), followed by methods A (13 positive samples) and C (5 positive samples); however, no isolation was obtained by method B. Fourteen Y. enterocolitica and 4 Yersinia intermedia strains were recovered by culture. All Y. enterocolitica 2/O:9 strains gave results related to virulence by phenotypic tests and exhibited the genotype virF þ myfA þ ail þ ystA þ . Two biotype 1A strains showed a genotype virF myfA ail þ ystA þ ystB þ . The 14 Y. enterocolitica strains isolated during this work plus one reference strain were separated into 11 genomic types by PFGE. This genomic heterogeneity of the isolates shows the diversity of Y. enterocolitica strains in our region. It is the first time that IMS was used to search Y. enterocolitica strains from naturally contaminated meat products.
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Escudero, María Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Materia
YERSINIA ENTEROCOLITICA
CULTURE METHODS
IMMUNOMAGNETIC SEPARATION
NESTED PCR
ASSOCIATED VIRULENCE
PFGE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158684

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network_name_str CONICET Digital (CONICET)
spelling Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCRLucero Estrada, Cecilia Stella MarysVelázquez, Lidia del CarmenFavier, Gabriela IsabelDi Genaro, Maria SilviaEscudero, María EstherYERSINIA ENTEROCOLITICACULTURE METHODSIMMUNOMAGNETIC SEPARATIONNESTED PCRASSOCIATED VIRULENCEPFGEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The prevalence of Yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (A), enrichment in modified Rappaport broth at 25 C (B), concentration by immunomagnetic separation (C) and yadA nested PCR (D). Furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (PFGE). A total of 238 samples were collected at retail level in the city of San Luis, Argentina, during the period 2007e2008. The highest Yersinia prevalence in meat products was observed by method D (92 positive samples), followed by methods A (13 positive samples) and C (5 positive samples); however, no isolation was obtained by method B. Fourteen Y. enterocolitica and 4 Yersinia intermedia strains were recovered by culture. All Y. enterocolitica 2/O:9 strains gave results related to virulence by phenotypic tests and exhibited the genotype virF þ myfA þ ail þ ystA þ . Two biotype 1A strains showed a genotype virF myfA ail þ ystA þ ystB þ . The 14 Y. enterocolitica strains isolated during this work plus one reference strain were separated into 11 genomic types by PFGE. This genomic heterogeneity of the isolates shows the diversity of Y. enterocolitica strains in our region. It is the first time that IMS was used to search Y. enterocolitica strains from naturally contaminated meat products.Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Escudero, María Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaElsevier2012-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158684Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Favier, Gabriela Isabel; Di Genaro, Maria Silvia; Escudero, María Esther; Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR; Elsevier; Food Microbiology; 30; 1; 5-2012; 157-1630740-0020CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0740002011002656info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fm.2011.10.014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:07Zoai:ri.conicet.gov.ar:11336/158684instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:08.114CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
title Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
spellingShingle Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
Lucero Estrada, Cecilia Stella Marys
YERSINIA ENTEROCOLITICA
CULTURE METHODS
IMMUNOMAGNETIC SEPARATION
NESTED PCR
ASSOCIATED VIRULENCE
PFGE
title_short Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
title_full Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
title_fullStr Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
title_full_unstemmed Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
title_sort Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR
dc.creator.none.fl_str_mv Lucero Estrada, Cecilia Stella Marys
Velázquez, Lidia del Carmen
Favier, Gabriela Isabel
Di Genaro, Maria Silvia
Escudero, María Esther
author Lucero Estrada, Cecilia Stella Marys
author_facet Lucero Estrada, Cecilia Stella Marys
Velázquez, Lidia del Carmen
Favier, Gabriela Isabel
Di Genaro, Maria Silvia
Escudero, María Esther
author_role author
author2 Velázquez, Lidia del Carmen
Favier, Gabriela Isabel
Di Genaro, Maria Silvia
Escudero, María Esther
author2_role author
author
author
author
dc.subject.none.fl_str_mv YERSINIA ENTEROCOLITICA
CULTURE METHODS
IMMUNOMAGNETIC SEPARATION
NESTED PCR
ASSOCIATED VIRULENCE
PFGE
topic YERSINIA ENTEROCOLITICA
CULTURE METHODS
IMMUNOMAGNETIC SEPARATION
NESTED PCR
ASSOCIATED VIRULENCE
PFGE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The prevalence of Yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (A), enrichment in modified Rappaport broth at 25 C (B), concentration by immunomagnetic separation (C) and yadA nested PCR (D). Furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (PFGE). A total of 238 samples were collected at retail level in the city of San Luis, Argentina, during the period 2007e2008. The highest Yersinia prevalence in meat products was observed by method D (92 positive samples), followed by methods A (13 positive samples) and C (5 positive samples); however, no isolation was obtained by method B. Fourteen Y. enterocolitica and 4 Yersinia intermedia strains were recovered by culture. All Y. enterocolitica 2/O:9 strains gave results related to virulence by phenotypic tests and exhibited the genotype virF þ myfA þ ail þ ystA þ . Two biotype 1A strains showed a genotype virF myfA ail þ ystA þ ystB þ . The 14 Y. enterocolitica strains isolated during this work plus one reference strain were separated into 11 genomic types by PFGE. This genomic heterogeneity of the isolates shows the diversity of Y. enterocolitica strains in our region. It is the first time that IMS was used to search Y. enterocolitica strains from naturally contaminated meat products.
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Escudero, María Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
description The prevalence of Yersinia enterocolitica in meat products was assessed by four methods: cold enrichment in trypticase soy broth (A), enrichment in modified Rappaport broth at 25 C (B), concentration by immunomagnetic separation (C) and yadA nested PCR (D). Furthermore, the pathogenic potentials of the isolates were established by phenotypic and genotypic tests, and their genomic relationships were determined by pulsed-field gel electrophoresis (PFGE). A total of 238 samples were collected at retail level in the city of San Luis, Argentina, during the period 2007e2008. The highest Yersinia prevalence in meat products was observed by method D (92 positive samples), followed by methods A (13 positive samples) and C (5 positive samples); however, no isolation was obtained by method B. Fourteen Y. enterocolitica and 4 Yersinia intermedia strains were recovered by culture. All Y. enterocolitica 2/O:9 strains gave results related to virulence by phenotypic tests and exhibited the genotype virF þ myfA þ ail þ ystA þ . Two biotype 1A strains showed a genotype virF myfA ail þ ystA þ ystB þ . The 14 Y. enterocolitica strains isolated during this work plus one reference strain were separated into 11 genomic types by PFGE. This genomic heterogeneity of the isolates shows the diversity of Y. enterocolitica strains in our region. It is the first time that IMS was used to search Y. enterocolitica strains from naturally contaminated meat products.
publishDate 2012
dc.date.none.fl_str_mv 2012-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158684
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Favier, Gabriela Isabel; Di Genaro, Maria Silvia; Escudero, María Esther; Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR; Elsevier; Food Microbiology; 30; 1; 5-2012; 157-163
0740-0020
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158684
identifier_str_mv Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Favier, Gabriela Isabel; Di Genaro, Maria Silvia; Escudero, María Esther; Detection of Yersinia spp. in meat products by enrichment culture, immunomagnetic separation and nested PCR; Elsevier; Food Microbiology; 30; 1; 5-2012; 157-163
0740-0020
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fm.2011.10.014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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