Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin

Autores
Lucero Estrada, Cecilia Stella Marys; Soria, José Miguel; Favier, Gabriela Isabel; Escudero, María Esther
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Yersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. This study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of Y. enterocolitica strains of different bioserotypes (B/O) isolated from foods and human samples in San Luis, Argentina. Strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, and pulsed-field gel electrophoresis (PFGE). Yersinia enterocolitica was detected in 9.2% of 380 samples, with a distribution of 10.6% (30/284) for food products and 5.2% (5/96) for human samples. Regarding the pathogenic potential, B1A strains of different serotypes were virF– ail–, of which 72.0% (13/18) were ystB+ with virulence-related phenotypic characteristics. Among B2/O:9 isolates, 75.0% (9/12) exhibited the genotype virF+ ail+ ystB– along with phenotypic traits associated with virulence; the same genotype was observed in 80.0% (4/5) of B3/O:3 and B3/O:5 strains. By PFGE, it was possible to separate Y. enterocolitica biotypes into 4 clonal groups (A to D) with 23 genomic types, generating a discriminatory index of 0.96. All isolates were susceptible to antimicrobials used for clinical treatment. This study highlights the presence of pathogenic bioserotypes and the high genomic diversity of the Y. enterocolitica strains isolated in our region.
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Soria, José Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina
Materia
Yersinia Enterocolitica
Foods
Human Samples
Pathogenic Potential
Pfge
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14699
Acceder
id CONICETDig_bdc02a36a517cf01904ee538a1f9114b
oai_identifier_str oai:ri.conicet.gov.ar:11336/14699
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human originLucero Estrada, Cecilia Stella MarysSoria, José MiguelFavier, Gabriela IsabelEscudero, María EstherYersinia EnterocoliticaFoodsHuman SamplesPathogenic PotentialPfgehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Yersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. This study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of Y. enterocolitica strains of different bioserotypes (B/O) isolated from foods and human samples in San Luis, Argentina. Strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, and pulsed-field gel electrophoresis (PFGE). Yersinia enterocolitica was detected in 9.2% of 380 samples, with a distribution of 10.6% (30/284) for food products and 5.2% (5/96) for human samples. Regarding the pathogenic potential, B1A strains of different serotypes were virF– ail–, of which 72.0% (13/18) were ystB+ with virulence-related phenotypic characteristics. Among B2/O:9 isolates, 75.0% (9/12) exhibited the genotype virF+ ail+ ystB– along with phenotypic traits associated with virulence; the same genotype was observed in 80.0% (4/5) of B3/O:3 and B3/O:5 strains. By PFGE, it was possible to separate Y. enterocolitica biotypes into 4 clonal groups (A to D) with 23 genomic types, generating a discriminatory index of 0.96. All isolates were susceptible to antimicrobials used for clinical treatment. This study highlights the presence of pathogenic bioserotypes and the high genomic diversity of the Y. enterocolitica strains isolated in our region.Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Soria, José Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; ArgentinaNatl Research Council Canada-n R C Research Press2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14699Lucero Estrada, Cecilia Stella Marys; Soria, José Miguel; Favier, Gabriela Isabel; Escudero, María Esther; Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin; Natl Research Council Canada-n R C Research Press; Canadian Journal Of Microbiology; 61; 11; 8-2015; 851-8600008-4166enginfo:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2015-0391#.WOKODfnhCJAinfo:eu-repo/semantics/altIdentifier/doi/10.1139/cjm-2015-0391info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:43:09Zoai:ri.conicet.gov.ar:11336/14699instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:43:09.839CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
title Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
spellingShingle Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
Lucero Estrada, Cecilia Stella Marys
Yersinia Enterocolitica
Foods
Human Samples
Pathogenic Potential
Pfge
title_short Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
title_full Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
title_fullStr Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
title_full_unstemmed Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
title_sort Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin
dc.creator.none.fl_str_mv Lucero Estrada, Cecilia Stella Marys
Soria, José Miguel
Favier, Gabriela Isabel
Escudero, María Esther
author Lucero Estrada, Cecilia Stella Marys
author_facet Lucero Estrada, Cecilia Stella Marys
Soria, José Miguel
Favier, Gabriela Isabel
Escudero, María Esther
author_role author
author2 Soria, José Miguel
Favier, Gabriela Isabel
Escudero, María Esther
author2_role author
author
author
dc.subject.none.fl_str_mv Yersinia Enterocolitica
Foods
Human Samples
Pathogenic Potential
Pfge
topic Yersinia Enterocolitica
Foods
Human Samples
Pathogenic Potential
Pfge
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Yersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. This study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of Y. enterocolitica strains of different bioserotypes (B/O) isolated from foods and human samples in San Luis, Argentina. Strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, and pulsed-field gel electrophoresis (PFGE). Yersinia enterocolitica was detected in 9.2% of 380 samples, with a distribution of 10.6% (30/284) for food products and 5.2% (5/96) for human samples. Regarding the pathogenic potential, B1A strains of different serotypes were virF– ail–, of which 72.0% (13/18) were ystB+ with virulence-related phenotypic characteristics. Among B2/O:9 isolates, 75.0% (9/12) exhibited the genotype virF+ ail+ ystB– along with phenotypic traits associated with virulence; the same genotype was observed in 80.0% (4/5) of B3/O:3 and B3/O:5 strains. By PFGE, it was possible to separate Y. enterocolitica biotypes into 4 clonal groups (A to D) with 23 genomic types, generating a discriminatory index of 0.96. All isolates were susceptible to antimicrobials used for clinical treatment. This study highlights the presence of pathogenic bioserotypes and the high genomic diversity of the Y. enterocolitica strains isolated in our region.
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Soria, José Miguel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Area Microbiologia; Argentina
description Yersinia enterocolitica is a food-borne pathogen that causes gastroenteritis with occasional postinfection sequels. This study was aimed to determinate the pathogenic potential, antimicrobial susceptibility, and genomic relationships of Y. enterocolitica strains of different bioserotypes (B/O) isolated from foods and human samples in San Luis, Argentina. Strains obtained by culture were bioserotyped and characterized by phenotypic and genotypic virulence markers, antimicrobial susceptibility, and pulsed-field gel electrophoresis (PFGE). Yersinia enterocolitica was detected in 9.2% of 380 samples, with a distribution of 10.6% (30/284) for food products and 5.2% (5/96) for human samples. Regarding the pathogenic potential, B1A strains of different serotypes were virF– ail–, of which 72.0% (13/18) were ystB+ with virulence-related phenotypic characteristics. Among B2/O:9 isolates, 75.0% (9/12) exhibited the genotype virF+ ail+ ystB– along with phenotypic traits associated with virulence; the same genotype was observed in 80.0% (4/5) of B3/O:3 and B3/O:5 strains. By PFGE, it was possible to separate Y. enterocolitica biotypes into 4 clonal groups (A to D) with 23 genomic types, generating a discriminatory index of 0.96. All isolates were susceptible to antimicrobials used for clinical treatment. This study highlights the presence of pathogenic bioserotypes and the high genomic diversity of the Y. enterocolitica strains isolated in our region.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14699
Lucero Estrada, Cecilia Stella Marys; Soria, José Miguel; Favier, Gabriela Isabel; Escudero, María Esther; Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin; Natl Research Council Canada-n R C Research Press; Canadian Journal Of Microbiology; 61; 11; 8-2015; 851-860
0008-4166
url http://hdl.handle.net/11336/14699
identifier_str_mv Lucero Estrada, Cecilia Stella Marys; Soria, José Miguel; Favier, Gabriela Isabel; Escudero, María Esther; Evaluation of the pathogenicity potential, antimicrobial susceptibility and genomic relations of Yersinia enterocolitica strains from food and human origin; Natl Research Council Canada-n R C Research Press; Canadian Journal Of Microbiology; 61; 11; 8-2015; 851-860
0008-4166
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2015-0391#.WOKODfnhCJA
info:eu-repo/semantics/altIdentifier/doi/10.1139/cjm-2015-0391
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
publisher.none.fl_str_mv Natl Research Council Canada-n R C Research Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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