Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.

Autores
Mastrodonato, Anna Chiara; Favier Gabriela Isabel; Lucero Estrada, Cecilia Stella Marys; Escudero María Esther
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Y. enterocolitica B1A comprise a heterogeneous group of strains that encompasses a wide variety of serotypes. They have been considered non-pathogenic microorganisms due to the lack of plasmid and chromosomal virulence determinants that characterize pathogenic strains; however, strains of this biotype are commonly reported not only from healthy individuals, but also from patients with gastrointestinal disorders. The comprehension of pathogenic mechanisms of B1A strains should focus on certain chromosomal virulence determinants associated to adhesion and invasion in intestinal cells (myfA and ail genes), production of heat-stable enterotoxin (ystB), some proteases (hreP) and iron chelating receptor (fepA), all of them related to growth and survival in host during infection. The synthesis of insecticidal toxins (tccC) is also considered a virulence determinant in Y. enterocolitica B1A. In this work, virulence-associated genes such as ystB (146 bp), myfA (272 bp), hreP (757 bp), fepA (438 bp) and tccC (1035 bp) were studied in 23 local Y. enterocolitica B1A strains of different origins (animal, food, environmental and human clinical samples) by PCR. Strains belonging to serotypes O:5 and O:7,8-8-8,19 (six isolates each), O:41,42-41,43 (four isolates), O:5-4,32-4,33 (three isolates), O:6,30-6,31 (two isolates), O:12,25-12,26 and NA (non-agglutinable/non-determined serotype) (one isolate each) were analyzed. DNA extraction was performed by the ―boiling‖ technique and the amplification products were revealed by agarose gel electrophoresis. The frequency of detection of these genes in decreasing order was: fepA and ystB (22/23), hreP (21/23), tccC (3/23) and myfA (1/23). Regarding the relationship between genes and serotypes, fepA, ystB and hreP genes were demonstrated in strains of all serotypes, meanwhile tccC was observed in O:41,42-41,43 and O:7,8-8-8,19 strains, and myfA was only detected in O:7,8-8-8,19 strains. The serotype O:7,8-8-8,19 was associated to the presence of all genes. Regarding the relationship between genes and strain sources, ystB, hreP and fepA were demonstrated in chicken samples (3 isolates), porcine products (five isolates), ground meat (six isolates), human clinical samples (three isolates), and wild boar, hake fillet and wastewater (one isolate each). The myfA gene was observed in porcine skin (one isolate) and tccC was present in porcine skin (two isolates) and wild boar (one isolate). Interestingly, human samples belonged to serotypes O:5 (two isolates) and O:7,8-8-8,19 (one isolate) showed to be carriers of most of the studied genes, except myfA and tccC. Our results suggest the existence of alternative virulence mechanisms in Y. enterocolitica B1A and that the pathogenic potential of this biotype might be strain-dependent.
Fil: Mastrodonato, Anna Chiara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Escudero María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
Materia
VIRULENCE MARKERS
YERSINIA ENTEROCOLITICA
BIOTYPE 1A
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199400

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network_name_str CONICET Digital (CONICET)
spelling Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.Mastrodonato, Anna ChiaraFavier Gabriela IsabelLucero Estrada, Cecilia Stella MarysEscudero María EstherVIRULENCE MARKERSYERSINIA ENTEROCOLITICABIOTYPE 1APCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Y. enterocolitica B1A comprise a heterogeneous group of strains that encompasses a wide variety of serotypes. They have been considered non-pathogenic microorganisms due to the lack of plasmid and chromosomal virulence determinants that characterize pathogenic strains; however, strains of this biotype are commonly reported not only from healthy individuals, but also from patients with gastrointestinal disorders. The comprehension of pathogenic mechanisms of B1A strains should focus on certain chromosomal virulence determinants associated to adhesion and invasion in intestinal cells (myfA and ail genes), production of heat-stable enterotoxin (ystB), some proteases (hreP) and iron chelating receptor (fepA), all of them related to growth and survival in host during infection. The synthesis of insecticidal toxins (tccC) is also considered a virulence determinant in Y. enterocolitica B1A. In this work, virulence-associated genes such as ystB (146 bp), myfA (272 bp), hreP (757 bp), fepA (438 bp) and tccC (1035 bp) were studied in 23 local Y. enterocolitica B1A strains of different origins (animal, food, environmental and human clinical samples) by PCR. Strains belonging to serotypes O:5 and O:7,8-8-8,19 (six isolates each), O:41,42-41,43 (four isolates), O:5-4,32-4,33 (three isolates), O:6,30-6,31 (two isolates), O:12,25-12,26 and NA (non-agglutinable/non-determined serotype) (one isolate each) were analyzed. DNA extraction was performed by the ―boiling‖ technique and the amplification products were revealed by agarose gel electrophoresis. The frequency of detection of these genes in decreasing order was: fepA and ystB (22/23), hreP (21/23), tccC (3/23) and myfA (1/23). Regarding the relationship between genes and serotypes, fepA, ystB and hreP genes were demonstrated in strains of all serotypes, meanwhile tccC was observed in O:41,42-41,43 and O:7,8-8-8,19 strains, and myfA was only detected in O:7,8-8-8,19 strains. The serotype O:7,8-8-8,19 was associated to the presence of all genes. Regarding the relationship between genes and strain sources, ystB, hreP and fepA were demonstrated in chicken samples (3 isolates), porcine products (five isolates), ground meat (six isolates), human clinical samples (three isolates), and wild boar, hake fillet and wastewater (one isolate each). The myfA gene was observed in porcine skin (one isolate) and tccC was present in porcine skin (two isolates) and wild boar (one isolate). Interestingly, human samples belonged to serotypes O:5 (two isolates) and O:7,8-8-8,19 (one isolate) showed to be carriers of most of the studied genes, except myfA and tccC. Our results suggest the existence of alternative virulence mechanisms in Y. enterocolitica B1A and that the pathogenic potential of this biotype might be strain-dependent.Fil: Mastrodonato, Anna Chiara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Escudero María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaXXXVII Reunión Científica Anual de la Sociedad de Biología de CuyoSan LuisArgentinaSociedad de Biología de CuyoSociedad de Biología de Cuyo2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectJornadaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199400Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2019/12/Libro-de-resumenes-2019.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:42Zoai:ri.conicet.gov.ar:11336/199400instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:42.75CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
title Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
spellingShingle Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
Mastrodonato, Anna Chiara
VIRULENCE MARKERS
YERSINIA ENTEROCOLITICA
BIOTYPE 1A
PCR
title_short Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
title_full Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
title_fullStr Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
title_full_unstemmed Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
title_sort Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.
dc.creator.none.fl_str_mv Mastrodonato, Anna Chiara
Favier Gabriela Isabel
Lucero Estrada, Cecilia Stella Marys
Escudero María Esther
author Mastrodonato, Anna Chiara
author_facet Mastrodonato, Anna Chiara
Favier Gabriela Isabel
Lucero Estrada, Cecilia Stella Marys
Escudero María Esther
author_role author
author2 Favier Gabriela Isabel
Lucero Estrada, Cecilia Stella Marys
Escudero María Esther
author2_role author
author
author
dc.subject.none.fl_str_mv VIRULENCE MARKERS
YERSINIA ENTEROCOLITICA
BIOTYPE 1A
PCR
topic VIRULENCE MARKERS
YERSINIA ENTEROCOLITICA
BIOTYPE 1A
PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Y. enterocolitica B1A comprise a heterogeneous group of strains that encompasses a wide variety of serotypes. They have been considered non-pathogenic microorganisms due to the lack of plasmid and chromosomal virulence determinants that characterize pathogenic strains; however, strains of this biotype are commonly reported not only from healthy individuals, but also from patients with gastrointestinal disorders. The comprehension of pathogenic mechanisms of B1A strains should focus on certain chromosomal virulence determinants associated to adhesion and invasion in intestinal cells (myfA and ail genes), production of heat-stable enterotoxin (ystB), some proteases (hreP) and iron chelating receptor (fepA), all of them related to growth and survival in host during infection. The synthesis of insecticidal toxins (tccC) is also considered a virulence determinant in Y. enterocolitica B1A. In this work, virulence-associated genes such as ystB (146 bp), myfA (272 bp), hreP (757 bp), fepA (438 bp) and tccC (1035 bp) were studied in 23 local Y. enterocolitica B1A strains of different origins (animal, food, environmental and human clinical samples) by PCR. Strains belonging to serotypes O:5 and O:7,8-8-8,19 (six isolates each), O:41,42-41,43 (four isolates), O:5-4,32-4,33 (three isolates), O:6,30-6,31 (two isolates), O:12,25-12,26 and NA (non-agglutinable/non-determined serotype) (one isolate each) were analyzed. DNA extraction was performed by the ―boiling‖ technique and the amplification products were revealed by agarose gel electrophoresis. The frequency of detection of these genes in decreasing order was: fepA and ystB (22/23), hreP (21/23), tccC (3/23) and myfA (1/23). Regarding the relationship between genes and serotypes, fepA, ystB and hreP genes were demonstrated in strains of all serotypes, meanwhile tccC was observed in O:41,42-41,43 and O:7,8-8-8,19 strains, and myfA was only detected in O:7,8-8-8,19 strains. The serotype O:7,8-8-8,19 was associated to the presence of all genes. Regarding the relationship between genes and strain sources, ystB, hreP and fepA were demonstrated in chicken samples (3 isolates), porcine products (five isolates), ground meat (six isolates), human clinical samples (three isolates), and wild boar, hake fillet and wastewater (one isolate each). The myfA gene was observed in porcine skin (one isolate) and tccC was present in porcine skin (two isolates) and wild boar (one isolate). Interestingly, human samples belonged to serotypes O:5 (two isolates) and O:7,8-8-8,19 (one isolate) showed to be carriers of most of the studied genes, except myfA and tccC. Our results suggest the existence of alternative virulence mechanisms in Y. enterocolitica B1A and that the pathogenic potential of this biotype might be strain-dependent.
Fil: Mastrodonato, Anna Chiara. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier Gabriela Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis. Universidad Nacional de San Luis. Facultad de Ciencias Físico Matemáticas y Naturales. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Escudero María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo
San Luis
Argentina
Sociedad de Biología de Cuyo
description Y. enterocolitica B1A comprise a heterogeneous group of strains that encompasses a wide variety of serotypes. They have been considered non-pathogenic microorganisms due to the lack of plasmid and chromosomal virulence determinants that characterize pathogenic strains; however, strains of this biotype are commonly reported not only from healthy individuals, but also from patients with gastrointestinal disorders. The comprehension of pathogenic mechanisms of B1A strains should focus on certain chromosomal virulence determinants associated to adhesion and invasion in intestinal cells (myfA and ail genes), production of heat-stable enterotoxin (ystB), some proteases (hreP) and iron chelating receptor (fepA), all of them related to growth and survival in host during infection. The synthesis of insecticidal toxins (tccC) is also considered a virulence determinant in Y. enterocolitica B1A. In this work, virulence-associated genes such as ystB (146 bp), myfA (272 bp), hreP (757 bp), fepA (438 bp) and tccC (1035 bp) were studied in 23 local Y. enterocolitica B1A strains of different origins (animal, food, environmental and human clinical samples) by PCR. Strains belonging to serotypes O:5 and O:7,8-8-8,19 (six isolates each), O:41,42-41,43 (four isolates), O:5-4,32-4,33 (three isolates), O:6,30-6,31 (two isolates), O:12,25-12,26 and NA (non-agglutinable/non-determined serotype) (one isolate each) were analyzed. DNA extraction was performed by the ―boiling‖ technique and the amplification products were revealed by agarose gel electrophoresis. The frequency of detection of these genes in decreasing order was: fepA and ystB (22/23), hreP (21/23), tccC (3/23) and myfA (1/23). Regarding the relationship between genes and serotypes, fepA, ystB and hreP genes were demonstrated in strains of all serotypes, meanwhile tccC was observed in O:41,42-41,43 and O:7,8-8-8,19 strains, and myfA was only detected in O:7,8-8-8,19 strains. The serotype O:7,8-8-8,19 was associated to the presence of all genes. Regarding the relationship between genes and strain sources, ystB, hreP and fepA were demonstrated in chicken samples (3 isolates), porcine products (five isolates), ground meat (six isolates), human clinical samples (three isolates), and wild boar, hake fillet and wastewater (one isolate each). The myfA gene was observed in porcine skin (one isolate) and tccC was present in porcine skin (two isolates) and wild boar (one isolate). Interestingly, human samples belonged to serotypes O:5 (two isolates) and O:7,8-8-8,19 (one isolate) showed to be carriers of most of the studied genes, except myfA and tccC. Our results suggest the existence of alternative virulence mechanisms in Y. enterocolitica B1A and that the pathogenic potential of this biotype might be strain-dependent.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Jornada
Book
http://purl.org/coar/resource_type/c_5794
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199400
Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199400
identifier_str_mv Assessment of virulence genotypic markers in Yersinia enterocolitica biotype 1A strains of different origins by PCR.; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2019
CONICET Digital
CONICET
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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dc.publisher.none.fl_str_mv Sociedad de Biología de Cuyo
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