Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR

Autores
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Materia
DNA EXTRACTION PROTOCOLS
COMPARISON
NESTED PCR
YADA GENE
YERSINIA ENTEROCOLITICA
MEAT FOOD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158394
Acceder
id CONICETDig_e7c9b5e4b947370e317af93f292de37b
oai_identifier_str oai:ri.conicet.gov.ar:11336/158394
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCRLucero Estrada, Cecilia Stella MarysVelázquez, Lidia del CarmenDi Genaro, Maria SilviaStefanini de Guzman, Ana Maria Teresa ValentinaDNA EXTRACTION PROTOCOLSCOMPARISONNESTED PCRYADA GENEYERSINIA ENTEROCOLITICAMEAT FOODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaElsevier Science2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158394Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-6420963-9969CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0963996906002067info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2006.11.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:40:29Zoai:ri.conicet.gov.ar:11336/158394instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:40:29.587CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
title Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
spellingShingle Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
Lucero Estrada, Cecilia Stella Marys
DNA EXTRACTION PROTOCOLS
COMPARISON
NESTED PCR
YADA GENE
YERSINIA ENTEROCOLITICA
MEAT FOOD
title_short Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
title_full Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
title_fullStr Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
title_full_unstemmed Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
title_sort Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
dc.creator.none.fl_str_mv Lucero Estrada, Cecilia Stella Marys
Velázquez, Lidia del Carmen
Di Genaro, Maria Silvia
Stefanini de Guzman, Ana Maria Teresa Valentina
author Lucero Estrada, Cecilia Stella Marys
author_facet Lucero Estrada, Cecilia Stella Marys
Velázquez, Lidia del Carmen
Di Genaro, Maria Silvia
Stefanini de Guzman, Ana Maria Teresa Valentina
author_role author
author2 Velázquez, Lidia del Carmen
Di Genaro, Maria Silvia
Stefanini de Guzman, Ana Maria Teresa Valentina
author2_role author
author
author
dc.subject.none.fl_str_mv DNA EXTRACTION PROTOCOLS
COMPARISON
NESTED PCR
YADA GENE
YERSINIA ENTEROCOLITICA
MEAT FOOD
topic DNA EXTRACTION PROTOCOLS
COMPARISON
NESTED PCR
YADA GENE
YERSINIA ENTEROCOLITICA
MEAT FOOD
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
description The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.
publishDate 2007
dc.date.none.fl_str_mv 2007-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158394
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-642
0963-9969
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158394
identifier_str_mv Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-642
0963-9969
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0963996906002067
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2006.11.008
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082896955179008
score 13.22299

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