Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR
- Autores
- Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina
- Año de publicación
- 2007
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina - Materia
-
DNA EXTRACTION PROTOCOLS
COMPARISON
NESTED PCR
YADA GENE
YERSINIA ENTEROCOLITICA
MEAT FOOD - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/158394
Ver los metadatos del registro completo
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Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCRLucero Estrada, Cecilia Stella MarysVelázquez, Lidia del CarmenDi Genaro, Maria SilviaStefanini de Guzman, Ana Maria Teresa ValentinaDNA EXTRACTION PROTOCOLSCOMPARISONNESTED PCRYADA GENEYERSINIA ENTEROCOLITICAMEAT FOODhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora.Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaElsevier Science2007-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158394Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-6420963-9969CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0963996906002067info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2006.11.008info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:48Zoai:ri.conicet.gov.ar:11336/158394instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:49.016CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
title |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
spellingShingle |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR Lucero Estrada, Cecilia Stella Marys DNA EXTRACTION PROTOCOLS COMPARISON NESTED PCR YADA GENE YERSINIA ENTEROCOLITICA MEAT FOOD |
title_short |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
title_full |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
title_fullStr |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
title_full_unstemmed |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
title_sort |
Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR |
dc.creator.none.fl_str_mv |
Lucero Estrada, Cecilia Stella Marys Velázquez, Lidia del Carmen Di Genaro, Maria Silvia Stefanini de Guzman, Ana Maria Teresa Valentina |
author |
Lucero Estrada, Cecilia Stella Marys |
author_facet |
Lucero Estrada, Cecilia Stella Marys Velázquez, Lidia del Carmen Di Genaro, Maria Silvia Stefanini de Guzman, Ana Maria Teresa Valentina |
author_role |
author |
author2 |
Velázquez, Lidia del Carmen Di Genaro, Maria Silvia Stefanini de Guzman, Ana Maria Teresa Valentina |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
DNA EXTRACTION PROTOCOLS COMPARISON NESTED PCR YADA GENE YERSINIA ENTEROCOLITICA MEAT FOOD |
topic |
DNA EXTRACTION PROTOCOLS COMPARISON NESTED PCR YADA GENE YERSINIA ENTEROCOLITICA MEAT FOOD |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora. Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina Fil: Velázquez, Lidia del Carmen. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina Fil: Di Genaro, Maria Silvia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina Fil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina |
description |
The objective of this work was to compare three different methods of DNA extraction from meat food, and to determine whether these methods removed inhibitors of nested PCR for pathogenic Yersinia enterocolitica detection. The amplification of the yadA gene from the DNA obtained from a pure Y. enterocolitica culture could be carried out with all the protocols. DNA amplification from the food samples was observed with two of the three tested protocols, which gave highly sensitive amplifications (detection limit 1 CFU/ml). These protocols detected a lower limit of 0.6 fg/μl of DNA extracted from Y. enterocolitica pure culture. We concluded that these protocols were able to eliminate satisfactorily the PCR inhibitors present in the foods. The nested PCR tested could be used satisfactorily in the investigation of pathogenic Y. enterocolitica in foods in the presence of a high background of microflora. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/158394 Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-642 0963-9969 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/158394 |
identifier_str_mv |
Lucero Estrada, Cecilia Stella Marys; Velázquez, Lidia del Carmen; Di Genaro, Maria Silvia; Stefanini de Guzman, Ana Maria Teresa Valentina; Comparison of DNA extraction methods for pathogenic Yersinia enterocolitica detection from meat food by nested PCR; Elsevier Science; Food Research International; 40; 5; 6-2007; 637-642 0963-9969 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0963996906002067 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.foodres.2006.11.008 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613461005303808 |
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13.070432 |