Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina

Autores
Lazarte Otero, Valeria Sabrina; Lucero Estrada, Cecilia Stella Marys; Favier, Gabriela Isabel; Velázquez, Lidia; Escudero, María Esther; Stefanini de Guzman, Ana Maria Teresa Valentina
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Detection limits and the survival of Yersinia enterocolitica in goat cheese were determined by culture and by nested polymerase chain reaction (PCR). Thirty goat cheese samples inoculated with 104 to 101 cfu/g Y. enterocolitica O:9 or O:3 strains were enriched for 0, 3 and 18h in trypticase soy broth (TSB), modified Rappaport broth and a formulated in our laboratory broth (FLB). The lowest detection limits were 1×103cfu/g by culture on Mac Conkey agar after 3h TSB and FLB enrichments, and 1×102cfu/g by nested PCR at 3h from all enrichment broths. Y. enterocolitica survival was studied in 20 goat cheese samples contaminated at levels of 1×106cfu/g and stored at 4° and 22C for 120 days. Y. enterocolitica was detected during 7 and 30 days at 22C and 4C, respectively. Total and fecal coliforms were recovered from microflora of goat cheese, but indigenous Y. enterocolitica was not detected.
Fil: Lazarte Otero, Valeria Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Materia
YERSINIA ENTEROCOLITICA
GOAT CHEESE
NESTED PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/158690
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, ArgentinaLazarte Otero, Valeria SabrinaLucero Estrada, Cecilia Stella MarysFavier, Gabriela IsabelVelázquez, LidiaEscudero, María EstherStefanini de Guzman, Ana Maria Teresa ValentinaYERSINIA ENTEROCOLITICAGOAT CHEESENESTED PCRhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Detection limits and the survival of Yersinia enterocolitica in goat cheese were determined by culture and by nested polymerase chain reaction (PCR). Thirty goat cheese samples inoculated with 104 to 101 cfu/g Y. enterocolitica O:9 or O:3 strains were enriched for 0, 3 and 18h in trypticase soy broth (TSB), modified Rappaport broth and a formulated in our laboratory broth (FLB). The lowest detection limits were 1×103cfu/g by culture on Mac Conkey agar after 3h TSB and FLB enrichments, and 1×102cfu/g by nested PCR at 3h from all enrichment broths. Y. enterocolitica survival was studied in 20 goat cheese samples contaminated at levels of 1×106cfu/g and stored at 4° and 22C for 120 days. Y. enterocolitica was detected during 7 and 30 days at 22C and 4C, respectively. Total and fecal coliforms were recovered from microflora of goat cheese, but indigenous Y. enterocolitica was not detected.Fil: Lazarte Otero, Valeria Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Velázquez, Lidia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaFil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; ArgentinaWiley Blackwell Publishing, Inc2010-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/158690Lazarte Otero, Valeria Sabrina; Lucero Estrada, Cecilia Stella Marys; Favier, Gabriela Isabel; Velázquez, Lidia; Escudero, María Esther; et al.; Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 30; 4; 7-2010; 982-9950149-6085CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1745-4565.2010.00256.xinfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1745-4565.2010.00256.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:09:11Zoai:ri.conicet.gov.ar:11336/158690instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:09:12.124CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
title Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
spellingShingle Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
Lazarte Otero, Valeria Sabrina
YERSINIA ENTEROCOLITICA
GOAT CHEESE
NESTED PCR
title_short Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
title_full Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
title_fullStr Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
title_full_unstemmed Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
title_sort Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina
dc.creator.none.fl_str_mv Lazarte Otero, Valeria Sabrina
Lucero Estrada, Cecilia Stella Marys
Favier, Gabriela Isabel
Velázquez, Lidia
Escudero, María Esther
Stefanini de Guzman, Ana Maria Teresa Valentina
author Lazarte Otero, Valeria Sabrina
author_facet Lazarte Otero, Valeria Sabrina
Lucero Estrada, Cecilia Stella Marys
Favier, Gabriela Isabel
Velázquez, Lidia
Escudero, María Esther
Stefanini de Guzman, Ana Maria Teresa Valentina
author_role author
author2 Lucero Estrada, Cecilia Stella Marys
Favier, Gabriela Isabel
Velázquez, Lidia
Escudero, María Esther
Stefanini de Guzman, Ana Maria Teresa Valentina
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv YERSINIA ENTEROCOLITICA
GOAT CHEESE
NESTED PCR
topic YERSINIA ENTEROCOLITICA
GOAT CHEESE
NESTED PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Detection limits and the survival of Yersinia enterocolitica in goat cheese were determined by culture and by nested polymerase chain reaction (PCR). Thirty goat cheese samples inoculated with 104 to 101 cfu/g Y. enterocolitica O:9 or O:3 strains were enriched for 0, 3 and 18h in trypticase soy broth (TSB), modified Rappaport broth and a formulated in our laboratory broth (FLB). The lowest detection limits were 1×103cfu/g by culture on Mac Conkey agar after 3h TSB and FLB enrichments, and 1×102cfu/g by nested PCR at 3h from all enrichment broths. Y. enterocolitica survival was studied in 20 goat cheese samples contaminated at levels of 1×106cfu/g and stored at 4° and 22C for 120 days. Y. enterocolitica was detected during 7 and 30 days at 22C and 4C, respectively. Total and fecal coliforms were recovered from microflora of goat cheese, but indigenous Y. enterocolitica was not detected.
Fil: Lazarte Otero, Valeria Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Lucero Estrada, Cecilia Stella Marys. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Favier, Gabriela Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Velázquez, Lidia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Escudero, María Esther. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
Fil: Stefanini de Guzman, Ana Maria Teresa Valentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Área Microbiología; Argentina
description Detection limits and the survival of Yersinia enterocolitica in goat cheese were determined by culture and by nested polymerase chain reaction (PCR). Thirty goat cheese samples inoculated with 104 to 101 cfu/g Y. enterocolitica O:9 or O:3 strains were enriched for 0, 3 and 18h in trypticase soy broth (TSB), modified Rappaport broth and a formulated in our laboratory broth (FLB). The lowest detection limits were 1×103cfu/g by culture on Mac Conkey agar after 3h TSB and FLB enrichments, and 1×102cfu/g by nested PCR at 3h from all enrichment broths. Y. enterocolitica survival was studied in 20 goat cheese samples contaminated at levels of 1×106cfu/g and stored at 4° and 22C for 120 days. Y. enterocolitica was detected during 7 and 30 days at 22C and 4C, respectively. Total and fecal coliforms were recovered from microflora of goat cheese, but indigenous Y. enterocolitica was not detected.
publishDate 2010
dc.date.none.fl_str_mv 2010-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/158690
Lazarte Otero, Valeria Sabrina; Lucero Estrada, Cecilia Stella Marys; Favier, Gabriela Isabel; Velázquez, Lidia; Escudero, María Esther; et al.; Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 30; 4; 7-2010; 982-995
0149-6085
CONICET Digital
CONICET
url http://hdl.handle.net/11336/158690
identifier_str_mv Lazarte Otero, Valeria Sabrina; Lucero Estrada, Cecilia Stella Marys; Favier, Gabriela Isabel; Velázquez, Lidia; Escudero, María Esther; et al.; Detection and survival of Yersinia enterocolitica in goat cheese produced in San Luis, Argentina; Wiley Blackwell Publishing, Inc; Journal of Food Safety; 30; 4; 7-2010; 982-995
0149-6085
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1745-4565.2010.00256.x
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1745-4565.2010.00256.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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