A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization

Autores
Mestre Citrinovitz, Ana Cecilia; Kleff, Veronika; Vallejo, Griselda; Winterhager, Elke; Saragüeta, Patricia Esther
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Progesterone receptor and estrogen receptor participate in growth and differentiation of the different rat decidual regions. Steroid hormone receptor antagonists were used to study steroid regulation of decidualization. Here we describe a suppressive interaction between progesterone receptor (onapristone) and estrogen receptor (ICI182780) antagonists and their relation to a rescue phenomenon with concomitant regulation of Hand2, Bmp2 and p-ERK1/2 during the early decidualization steps. Phenotypes of decidua development produced by antagonist treatments were characterized by morphology, proliferation, differentiation, angiogenesis and expression of signaling molecules. We found that suppression of progesterone receptor activity by onapristone treatment resulted in resorption of the implantation sites with concomitant decrease in progesterone and estrogen receptors, PCNA, KI67 antigen, DESMIN, CCND3, CX43, Prl8a2, and signaling players such as transcription factor Hand2, Bmp2 mRNAs and p-ERK1/2. Moreover, FGF-2 and Vegfa increased as a consequence of onapristone treatment. Implantation sites from antagonist of estrogen receptor treated rats developed all decidual regions, but showed an anomalous blood vessel formation at the mesometrial part of the decidua. The deleterious effect of onapristone was partially counteracted by the impairment of estrogen receptor activity with rescue of expression levels of hormone steroid receptors, proliferation and differentiation markers, and the induction of a probably compensatory increase in signaling molecules Hand2, Bmp2 and ERK1/2 activation compared to oil treated controls. This novel drug interaction during decidualization could be applied to pathological endometrial cell proliferation processes to improve therapies using steroid hormone receptor targets.
Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Kleff, Veronika. Universaetsklinikum Duisburg-Essen. Institut für Anatomie; Alemania
Fil: Vallejo, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Winterhager, Elke . Universaetsklinikum Duisburg-Essen. Institut Für Molekularbiologie; Alemania
Fil: Saragüeta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
ENDOMETRIUM
PROGESTERONE RECEPTOR
ESTROGEN RECEPTOR
DECIDUALIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/8807
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/8807
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualizationMestre Citrinovitz, Ana CeciliaKleff, VeronikaVallejo, GriseldaWinterhager, Elke Saragüeta, Patricia EstherENDOMETRIUMPROGESTERONE RECEPTORESTROGEN RECEPTORDECIDUALIZATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Progesterone receptor and estrogen receptor participate in growth and differentiation of the different rat decidual regions. Steroid hormone receptor antagonists were used to study steroid regulation of decidualization. Here we describe a suppressive interaction between progesterone receptor (onapristone) and estrogen receptor (ICI182780) antagonists and their relation to a rescue phenomenon with concomitant regulation of Hand2, Bmp2 and p-ERK1/2 during the early decidualization steps. Phenotypes of decidua development produced by antagonist treatments were characterized by morphology, proliferation, differentiation, angiogenesis and expression of signaling molecules. We found that suppression of progesterone receptor activity by onapristone treatment resulted in resorption of the implantation sites with concomitant decrease in progesterone and estrogen receptors, PCNA, KI67 antigen, DESMIN, CCND3, CX43, Prl8a2, and signaling players such as transcription factor Hand2, Bmp2 mRNAs and p-ERK1/2. Moreover, FGF-2 and Vegfa increased as a consequence of onapristone treatment. Implantation sites from antagonist of estrogen receptor treated rats developed all decidual regions, but showed an anomalous blood vessel formation at the mesometrial part of the decidua. The deleterious effect of onapristone was partially counteracted by the impairment of estrogen receptor activity with rescue of expression levels of hormone steroid receptors, proliferation and differentiation markers, and the induction of a probably compensatory increase in signaling molecules Hand2, Bmp2 and ERK1/2 activation compared to oil treated controls. This novel drug interaction during decidualization could be applied to pathological endometrial cell proliferation processes to improve therapies using steroid hormone receptor targets.Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Kleff, Veronika. Universaetsklinikum Duisburg-Essen. Institut für Anatomie; AlemaniaFil: Vallejo, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Winterhager, Elke . Universaetsklinikum Duisburg-Essen. Institut Für Molekularbiologie; AlemaniaFil: Saragüeta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaPublic Library Of Science2015-04-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/8807Mestre Citrinovitz, Ana Cecilia; Kleff, Veronika; Vallejo, Griselda; Winterhager, Elke ; Saragüeta, Patricia Esther; A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization; Public Library Of Science; Plos One; 10; 4; 21-4-2015; 1-201932-62031932-6203enginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0124756info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405574/info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124756info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:16Zoai:ri.conicet.gov.ar:11336/8807instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:17.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
title A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
spellingShingle A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
Mestre Citrinovitz, Ana Cecilia
ENDOMETRIUM
PROGESTERONE RECEPTOR
ESTROGEN RECEPTOR
DECIDUALIZATION
title_short A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
title_full A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
title_fullStr A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
title_full_unstemmed A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
title_sort A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization
dc.creator.none.fl_str_mv Mestre Citrinovitz, Ana Cecilia
Kleff, Veronika
Vallejo, Griselda
Winterhager, Elke
Saragüeta, Patricia Esther
author Mestre Citrinovitz, Ana Cecilia
author_facet Mestre Citrinovitz, Ana Cecilia
Kleff, Veronika
Vallejo, Griselda
Winterhager, Elke
Saragüeta, Patricia Esther
author_role author
author2 Kleff, Veronika
Vallejo, Griselda
Winterhager, Elke
Saragüeta, Patricia Esther
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENDOMETRIUM
PROGESTERONE RECEPTOR
ESTROGEN RECEPTOR
DECIDUALIZATION
topic ENDOMETRIUM
PROGESTERONE RECEPTOR
ESTROGEN RECEPTOR
DECIDUALIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Progesterone receptor and estrogen receptor participate in growth and differentiation of the different rat decidual regions. Steroid hormone receptor antagonists were used to study steroid regulation of decidualization. Here we describe a suppressive interaction between progesterone receptor (onapristone) and estrogen receptor (ICI182780) antagonists and their relation to a rescue phenomenon with concomitant regulation of Hand2, Bmp2 and p-ERK1/2 during the early decidualization steps. Phenotypes of decidua development produced by antagonist treatments were characterized by morphology, proliferation, differentiation, angiogenesis and expression of signaling molecules. We found that suppression of progesterone receptor activity by onapristone treatment resulted in resorption of the implantation sites with concomitant decrease in progesterone and estrogen receptors, PCNA, KI67 antigen, DESMIN, CCND3, CX43, Prl8a2, and signaling players such as transcription factor Hand2, Bmp2 mRNAs and p-ERK1/2. Moreover, FGF-2 and Vegfa increased as a consequence of onapristone treatment. Implantation sites from antagonist of estrogen receptor treated rats developed all decidual regions, but showed an anomalous blood vessel formation at the mesometrial part of the decidua. The deleterious effect of onapristone was partially counteracted by the impairment of estrogen receptor activity with rescue of expression levels of hormone steroid receptors, proliferation and differentiation markers, and the induction of a probably compensatory increase in signaling molecules Hand2, Bmp2 and ERK1/2 activation compared to oil treated controls. This novel drug interaction during decidualization could be applied to pathological endometrial cell proliferation processes to improve therapies using steroid hormone receptor targets.
Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Kleff, Veronika. Universaetsklinikum Duisburg-Essen. Institut für Anatomie; Alemania
Fil: Vallejo, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Winterhager, Elke . Universaetsklinikum Duisburg-Essen. Institut Für Molekularbiologie; Alemania
Fil: Saragüeta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Progesterone receptor and estrogen receptor participate in growth and differentiation of the different rat decidual regions. Steroid hormone receptor antagonists were used to study steroid regulation of decidualization. Here we describe a suppressive interaction between progesterone receptor (onapristone) and estrogen receptor (ICI182780) antagonists and their relation to a rescue phenomenon with concomitant regulation of Hand2, Bmp2 and p-ERK1/2 during the early decidualization steps. Phenotypes of decidua development produced by antagonist treatments were characterized by morphology, proliferation, differentiation, angiogenesis and expression of signaling molecules. We found that suppression of progesterone receptor activity by onapristone treatment resulted in resorption of the implantation sites with concomitant decrease in progesterone and estrogen receptors, PCNA, KI67 antigen, DESMIN, CCND3, CX43, Prl8a2, and signaling players such as transcription factor Hand2, Bmp2 mRNAs and p-ERK1/2. Moreover, FGF-2 and Vegfa increased as a consequence of onapristone treatment. Implantation sites from antagonist of estrogen receptor treated rats developed all decidual regions, but showed an anomalous blood vessel formation at the mesometrial part of the decidua. The deleterious effect of onapristone was partially counteracted by the impairment of estrogen receptor activity with rescue of expression levels of hormone steroid receptors, proliferation and differentiation markers, and the induction of a probably compensatory increase in signaling molecules Hand2, Bmp2 and ERK1/2 activation compared to oil treated controls. This novel drug interaction during decidualization could be applied to pathological endometrial cell proliferation processes to improve therapies using steroid hormone receptor targets.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/8807
Mestre Citrinovitz, Ana Cecilia; Kleff, Veronika; Vallejo, Griselda; Winterhager, Elke ; Saragüeta, Patricia Esther; A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization; Public Library Of Science; Plos One; 10; 4; 21-4-2015; 1-20
1932-6203
1932-6203
url http://hdl.handle.net/11336/8807
identifier_str_mv Mestre Citrinovitz, Ana Cecilia; Kleff, Veronika; Vallejo, Griselda; Winterhager, Elke ; Saragüeta, Patricia Esther; A suppressive antagonism evidences Progesterone and Estrogen receptor pathway interaction with concomitant regulation of Hand2, Bmp2 and ERK during early decidualization; Public Library Of Science; Plos One; 10; 4; 21-4-2015; 1-20
1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0124756
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4405574/
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0124756
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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