Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas

Autores
Bianchi, Carolina Paula; Meikle, A.; Sartore, I.; Gonzalez, F.; Aba, Marcelo Alfredo
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Estrogen receptor- (ER) and progesterone receptor (PR) were characterized in different endometrial cell types as luminal and glandular epithelium and stroma during the follicular (FP) and the luteal phase (LP) in llamas. Animals were examined daily by transrectal ultrasonography for the determination of the presence of an ovulatory follicle and ovulation was immediately induced by a GnRH injection (Day 0). Endometrial samples were obtained by transcervical biopsies from the left uterine horn on Day 0 (FP) and 9 days after the GnRH injection (Day 9, LP). Blood samples were collected on these days for estradiol 17 and progesterone determination by RIA. An immunohistochemical technique was used to visualize ER and PR immunostaining which was then analyzed by two independent observers. Total positive area and average staining for ER were affected by the phase of the ovarian activity: in the three cell types there was more positive area and intense staining during the FP than during the LP. Similar findings were observed for PR, more positive stained areas were found during the FP than during the LP in the epithelia. In addition, the three cell types had more intense staining during the FP than during the LP. An effect of the cell type for ER and PR was observed; epithelia (luminal and glandular) had more positive stained areas and greater intensity than stromal cells. In conclusion, the results of the present study suggest that in llamas, like in other ruminants, estradiol has a stimulatory effect while progesterone downregulates the ERand PR and that the receptor is cell type specific.
Fil: Bianchi, Carolina Paula. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina
Fil: Meikle, A.. Universidad de Concepción del Uruguay; Argentina
Fil: Sartore, I.. Universidad de Concepción del Uruguay; Argentina
Fil: Gonzalez, F.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Fil: Aba, Marcelo Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Materia
Llamas
Estrogen Receptor
Progesterone Receptor
Ovarian Phases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/239869
Acceder
id CONICETDig_55bfe850e13c201132f68257f51bc706
oai_identifier_str oai:ri.conicet.gov.ar:11336/239869
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamasBianchi, Carolina PaulaMeikle, A.Sartore, I.Gonzalez, F.Aba, Marcelo AlfredoLlamasEstrogen ReceptorProgesterone ReceptorOvarian Phaseshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Estrogen receptor- (ER) and progesterone receptor (PR) were characterized in different endometrial cell types as luminal and glandular epithelium and stroma during the follicular (FP) and the luteal phase (LP) in llamas. Animals were examined daily by transrectal ultrasonography for the determination of the presence of an ovulatory follicle and ovulation was immediately induced by a GnRH injection (Day 0). Endometrial samples were obtained by transcervical biopsies from the left uterine horn on Day 0 (FP) and 9 days after the GnRH injection (Day 9, LP). Blood samples were collected on these days for estradiol 17 and progesterone determination by RIA. An immunohistochemical technique was used to visualize ER and PR immunostaining which was then analyzed by two independent observers. Total positive area and average staining for ER were affected by the phase of the ovarian activity: in the three cell types there was more positive area and intense staining during the FP than during the LP. Similar findings were observed for PR, more positive stained areas were found during the FP than during the LP in the epithelia. In addition, the three cell types had more intense staining during the FP than during the LP. An effect of the cell type for ER and PR was observed; epithelia (luminal and glandular) had more positive stained areas and greater intensity than stromal cells. In conclusion, the results of the present study suggest that in llamas, like in other ruminants, estradiol has a stimulatory effect while progesterone downregulates the ERand PR and that the receptor is cell type specific.Fil: Bianchi, Carolina Paula. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; ArgentinaFil: Meikle, A.. Universidad de Concepción del Uruguay; ArgentinaFil: Sartore, I.. Universidad de Concepción del Uruguay; ArgentinaFil: Gonzalez, F.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; ArgentinaFil: Aba, Marcelo Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; ArgentinaElsevier Science2007-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/239869Bianchi, Carolina Paula; Meikle, A.; Sartore, I.; Gonzalez, F.; Aba, Marcelo Alfredo; Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas; Elsevier Science; Animal Reproduction Science; 99; 1-2; 5-2007; 117-1260378-4320CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432006002156info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2006.04.042info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:08Zoai:ri.conicet.gov.ar:11336/239869instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:08.714CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
title Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
spellingShingle Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
Bianchi, Carolina Paula
Llamas
Estrogen Receptor
Progesterone Receptor
Ovarian Phases
title_short Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
title_full Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
title_fullStr Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
title_full_unstemmed Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
title_sort Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas
dc.creator.none.fl_str_mv Bianchi, Carolina Paula
Meikle, A.
Sartore, I.
Gonzalez, F.
Aba, Marcelo Alfredo
author Bianchi, Carolina Paula
author_facet Bianchi, Carolina Paula
Meikle, A.
Sartore, I.
Gonzalez, F.
Aba, Marcelo Alfredo
author_role author
author2 Meikle, A.
Sartore, I.
Gonzalez, F.
Aba, Marcelo Alfredo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Llamas
Estrogen Receptor
Progesterone Receptor
Ovarian Phases
topic Llamas
Estrogen Receptor
Progesterone Receptor
Ovarian Phases
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Estrogen receptor- (ER) and progesterone receptor (PR) were characterized in different endometrial cell types as luminal and glandular epithelium and stroma during the follicular (FP) and the luteal phase (LP) in llamas. Animals were examined daily by transrectal ultrasonography for the determination of the presence of an ovulatory follicle and ovulation was immediately induced by a GnRH injection (Day 0). Endometrial samples were obtained by transcervical biopsies from the left uterine horn on Day 0 (FP) and 9 days after the GnRH injection (Day 9, LP). Blood samples were collected on these days for estradiol 17 and progesterone determination by RIA. An immunohistochemical technique was used to visualize ER and PR immunostaining which was then analyzed by two independent observers. Total positive area and average staining for ER were affected by the phase of the ovarian activity: in the three cell types there was more positive area and intense staining during the FP than during the LP. Similar findings were observed for PR, more positive stained areas were found during the FP than during the LP in the epithelia. In addition, the three cell types had more intense staining during the FP than during the LP. An effect of the cell type for ER and PR was observed; epithelia (luminal and glandular) had more positive stained areas and greater intensity than stromal cells. In conclusion, the results of the present study suggest that in llamas, like in other ruminants, estradiol has a stimulatory effect while progesterone downregulates the ERand PR and that the receptor is cell type specific.
Fil: Bianchi, Carolina Paula. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina
Fil: Meikle, A.. Universidad de Concepción del Uruguay; Argentina
Fil: Sartore, I.. Universidad de Concepción del Uruguay; Argentina
Fil: Gonzalez, F.. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
Fil: Aba, Marcelo Alfredo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Endocrinología; Argentina
description Estrogen receptor- (ER) and progesterone receptor (PR) were characterized in different endometrial cell types as luminal and glandular epithelium and stroma during the follicular (FP) and the luteal phase (LP) in llamas. Animals were examined daily by transrectal ultrasonography for the determination of the presence of an ovulatory follicle and ovulation was immediately induced by a GnRH injection (Day 0). Endometrial samples were obtained by transcervical biopsies from the left uterine horn on Day 0 (FP) and 9 days after the GnRH injection (Day 9, LP). Blood samples were collected on these days for estradiol 17 and progesterone determination by RIA. An immunohistochemical technique was used to visualize ER and PR immunostaining which was then analyzed by two independent observers. Total positive area and average staining for ER were affected by the phase of the ovarian activity: in the three cell types there was more positive area and intense staining during the FP than during the LP. Similar findings were observed for PR, more positive stained areas were found during the FP than during the LP in the epithelia. In addition, the three cell types had more intense staining during the FP than during the LP. An effect of the cell type for ER and PR was observed; epithelia (luminal and glandular) had more positive stained areas and greater intensity than stromal cells. In conclusion, the results of the present study suggest that in llamas, like in other ruminants, estradiol has a stimulatory effect while progesterone downregulates the ERand PR and that the receptor is cell type specific.
publishDate 2007
dc.date.none.fl_str_mv 2007-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/239869
Bianchi, Carolina Paula; Meikle, A.; Sartore, I.; Gonzalez, F.; Aba, Marcelo Alfredo; Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas; Elsevier Science; Animal Reproduction Science; 99; 1-2; 5-2007; 117-126
0378-4320
CONICET Digital
CONICET
url http://hdl.handle.net/11336/239869
identifier_str_mv Bianchi, Carolina Paula; Meikle, A.; Sartore, I.; Gonzalez, F.; Aba, Marcelo Alfredo; Uterine estrogen receptor alpha and progesterone receptor during the follicular and luteal phase in llamas; Elsevier Science; Animal Reproduction Science; 99; 1-2; 5-2007; 117-126
0378-4320
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378432006002156
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.anireprosci.2006.04.042
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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