Ovarian steroid receptors and activated MAPK in the regional decidualization in rats

Autores
Vallejo, Griselda; Mestre Citrinovitz, Ana Cecilia; Moenckendiek. Verena; Grümmer, Ruth; Winterhager, Elke; Saragueta, Patricia Esther
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Though the decidua serves a critical function in implantation, the hormonal regulated pathway in decidualization is still elusive. Here we describe in detail the regional distribution and the effects of progesterone receptors (PGR), estrogen receptors (ESR), and MAPK activation on decidualization. We showed an increase in PGR A, PGR B, ESR1, and phosphorylated MAPK3-1 proteins (p-MAPK3-1), but not in ESR2, in the decidual tissue up to Day 8 of pregnancy. PGR was predominantly found in the nuclei of mesometrial decidual cells and of undifferentiated stromal cells where it colocalizes with ESR2 and ESR1. In the antimesometrial decidua, all the receptors showed cytoplasmic localization. MAPK was activated exclusively in undifferentiated stromal cells of the junctional zone between the antimesometrial and mesometrial decidua and at the border of the antimesometrial decidua. Treatment with the progesterone antagonist onapristone and/or the estrogen antagonist faslodex reduced the extent of decidual tissue and downregulated the levels of PGR and ESR1. The expression level of ESR2 was affected only by the progesterone receptor antagonist, while neither the antiprogestin nor the antiestrogen significantly modified the p-MAPK3-1 level. The inhibition of MAPK3-1 phosphorylation by PD98059 impaired the extent of decidualization and the closure reaction of the implantation chamber, and significantly downregulated ESR1. These results confirm a role of both steroid receptors in the growth and differentiation of the different decidual regions and suggest a new function for p-MAPK3-1 in regulating expression levels of ESR1, thereby maintaining the proliferation capacity of stromal cells and limiting the differentiation process in specified regions of decidual tissues.
Fil: Vallejo, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Moenckendiek. Verena. Universitat Duisburg - Essen; Alemania
Fil: Grümmer, Ruth. Universitat Duisburg - Essen; Alemania
Fil: Winterhager, Elke. Universitat Duisburg - Essen; Alemania
Fil: Saragueta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
Endometrium
Progesterone
Decidualization
Reproductive Tract
Rats
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14294
Acceder
id CONICETDig_2dd898fde7302e7cc87b0aac06e8fb6f
oai_identifier_str oai:ri.conicet.gov.ar:11336/14294
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ovarian steroid receptors and activated MAPK in the regional decidualization in ratsVallejo, GriseldaMestre Citrinovitz, Ana CeciliaMoenckendiek. VerenaGrümmer, RuthWinterhager, ElkeSaragueta, Patricia EstherEndometriumProgesteroneDecidualizationReproductive TractRatshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Though the decidua serves a critical function in implantation, the hormonal regulated pathway in decidualization is still elusive. Here we describe in detail the regional distribution and the effects of progesterone receptors (PGR), estrogen receptors (ESR), and MAPK activation on decidualization. We showed an increase in PGR A, PGR B, ESR1, and phosphorylated MAPK3-1 proteins (p-MAPK3-1), but not in ESR2, in the decidual tissue up to Day 8 of pregnancy. PGR was predominantly found in the nuclei of mesometrial decidual cells and of undifferentiated stromal cells where it colocalizes with ESR2 and ESR1. In the antimesometrial decidua, all the receptors showed cytoplasmic localization. MAPK was activated exclusively in undifferentiated stromal cells of the junctional zone between the antimesometrial and mesometrial decidua and at the border of the antimesometrial decidua. Treatment with the progesterone antagonist onapristone and/or the estrogen antagonist faslodex reduced the extent of decidual tissue and downregulated the levels of PGR and ESR1. The expression level of ESR2 was affected only by the progesterone receptor antagonist, while neither the antiprogestin nor the antiestrogen significantly modified the p-MAPK3-1 level. The inhibition of MAPK3-1 phosphorylation by PD98059 impaired the extent of decidualization and the closure reaction of the implantation chamber, and significantly downregulated ESR1. These results confirm a role of both steroid receptors in the growth and differentiation of the different decidual regions and suggest a new function for p-MAPK3-1 in regulating expression levels of ESR1, thereby maintaining the proliferation capacity of stromal cells and limiting the differentiation process in specified regions of decidual tissues.Fil: Vallejo, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Moenckendiek. Verena. Universitat Duisburg - Essen; AlemaniaFil: Grümmer, Ruth. Universitat Duisburg - Essen; AlemaniaFil: Winterhager, Elke. Universitat Duisburg - Essen; AlemaniaFil: Saragueta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaSociety For The Study Of Reproduction2011-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14294Vallejo, Griselda; Mestre Citrinovitz, Ana Cecilia; Moenckendiek. Verena; Grümmer, Ruth; Winterhager, Elke; et al.; Ovarian steroid receptors and activated MAPK in the regional decidualization in rats; Society For The Study Of Reproduction; Biology Of Reproduction; 84; 5; 5-2011; 1063-10710006-33631529-7268enginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/2530470/Ovarianinfo:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.110.085928info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:23Zoai:ri.conicet.gov.ar:11336/14294instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:24.198CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
title Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
spellingShingle Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
Vallejo, Griselda
Endometrium
Progesterone
Decidualization
Reproductive Tract
Rats
title_short Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
title_full Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
title_fullStr Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
title_full_unstemmed Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
title_sort Ovarian steroid receptors and activated MAPK in the regional decidualization in rats
dc.creator.none.fl_str_mv Vallejo, Griselda
Mestre Citrinovitz, Ana Cecilia
Moenckendiek. Verena
Grümmer, Ruth
Winterhager, Elke
Saragueta, Patricia Esther
author Vallejo, Griselda
author_facet Vallejo, Griselda
Mestre Citrinovitz, Ana Cecilia
Moenckendiek. Verena
Grümmer, Ruth
Winterhager, Elke
Saragueta, Patricia Esther
author_role author
author2 Mestre Citrinovitz, Ana Cecilia
Moenckendiek. Verena
Grümmer, Ruth
Winterhager, Elke
Saragueta, Patricia Esther
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Endometrium
Progesterone
Decidualization
Reproductive Tract
Rats
topic Endometrium
Progesterone
Decidualization
Reproductive Tract
Rats
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Though the decidua serves a critical function in implantation, the hormonal regulated pathway in decidualization is still elusive. Here we describe in detail the regional distribution and the effects of progesterone receptors (PGR), estrogen receptors (ESR), and MAPK activation on decidualization. We showed an increase in PGR A, PGR B, ESR1, and phosphorylated MAPK3-1 proteins (p-MAPK3-1), but not in ESR2, in the decidual tissue up to Day 8 of pregnancy. PGR was predominantly found in the nuclei of mesometrial decidual cells and of undifferentiated stromal cells where it colocalizes with ESR2 and ESR1. In the antimesometrial decidua, all the receptors showed cytoplasmic localization. MAPK was activated exclusively in undifferentiated stromal cells of the junctional zone between the antimesometrial and mesometrial decidua and at the border of the antimesometrial decidua. Treatment with the progesterone antagonist onapristone and/or the estrogen antagonist faslodex reduced the extent of decidual tissue and downregulated the levels of PGR and ESR1. The expression level of ESR2 was affected only by the progesterone receptor antagonist, while neither the antiprogestin nor the antiestrogen significantly modified the p-MAPK3-1 level. The inhibition of MAPK3-1 phosphorylation by PD98059 impaired the extent of decidualization and the closure reaction of the implantation chamber, and significantly downregulated ESR1. These results confirm a role of both steroid receptors in the growth and differentiation of the different decidual regions and suggest a new function for p-MAPK3-1 in regulating expression levels of ESR1, thereby maintaining the proliferation capacity of stromal cells and limiting the differentiation process in specified regions of decidual tissues.
Fil: Vallejo, Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Mestre Citrinovitz, Ana Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Moenckendiek. Verena. Universitat Duisburg - Essen; Alemania
Fil: Grümmer, Ruth. Universitat Duisburg - Essen; Alemania
Fil: Winterhager, Elke. Universitat Duisburg - Essen; Alemania
Fil: Saragueta, Patricia Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Though the decidua serves a critical function in implantation, the hormonal regulated pathway in decidualization is still elusive. Here we describe in detail the regional distribution and the effects of progesterone receptors (PGR), estrogen receptors (ESR), and MAPK activation on decidualization. We showed an increase in PGR A, PGR B, ESR1, and phosphorylated MAPK3-1 proteins (p-MAPK3-1), but not in ESR2, in the decidual tissue up to Day 8 of pregnancy. PGR was predominantly found in the nuclei of mesometrial decidual cells and of undifferentiated stromal cells where it colocalizes with ESR2 and ESR1. In the antimesometrial decidua, all the receptors showed cytoplasmic localization. MAPK was activated exclusively in undifferentiated stromal cells of the junctional zone between the antimesometrial and mesometrial decidua and at the border of the antimesometrial decidua. Treatment with the progesterone antagonist onapristone and/or the estrogen antagonist faslodex reduced the extent of decidual tissue and downregulated the levels of PGR and ESR1. The expression level of ESR2 was affected only by the progesterone receptor antagonist, while neither the antiprogestin nor the antiestrogen significantly modified the p-MAPK3-1 level. The inhibition of MAPK3-1 phosphorylation by PD98059 impaired the extent of decidualization and the closure reaction of the implantation chamber, and significantly downregulated ESR1. These results confirm a role of both steroid receptors in the growth and differentiation of the different decidual regions and suggest a new function for p-MAPK3-1 in regulating expression levels of ESR1, thereby maintaining the proliferation capacity of stromal cells and limiting the differentiation process in specified regions of decidual tissues.
publishDate 2011
dc.date.none.fl_str_mv 2011-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14294
Vallejo, Griselda; Mestre Citrinovitz, Ana Cecilia; Moenckendiek. Verena; Grümmer, Ruth; Winterhager, Elke; et al.; Ovarian steroid receptors and activated MAPK in the regional decidualization in rats; Society For The Study Of Reproduction; Biology Of Reproduction; 84; 5; 5-2011; 1063-1071
0006-3363
1529-7268
url http://hdl.handle.net/11336/14294
identifier_str_mv Vallejo, Griselda; Mestre Citrinovitz, Ana Cecilia; Moenckendiek. Verena; Grümmer, Ruth; Winterhager, Elke; et al.; Ovarian steroid receptors and activated MAPK in the regional decidualization in rats; Society For The Study Of Reproduction; Biology Of Reproduction; 84; 5; 5-2011; 1063-1071
0006-3363
1529-7268
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/biolreprod/article/2530470/Ovarian
info:eu-repo/semantics/altIdentifier/doi/10.1095/biolreprod.110.085928
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society For The Study Of Reproduction
publisher.none.fl_str_mv Society For The Study Of Reproduction
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268728249548800
score 12.885934

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