Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom

Autores
Canevari, José; Ceballos, Laura; Sanabria, Rodrigo Eduardo Fabrizio; Romero, Jorge Roberto; Olaechea, Fermín; Ortiz, Pedro; Cabrera, María; Gayo, Valeria; Fairweather, Ian; Lanusse, Carlos Edmundo; Alvarez, Luis Ignacio
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The main goal of the current work was to develop and validate an in vitro fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke, Fasciola hepatica. Fluke eggs (200/ml, n ¼ 5) from six different isolates were used in the current experimental work. They were obtained from different geographical locations and named Cullompton (UK), CEDIVE (Chascomus, Argentina), INTA-Bariloche (Bariloche, Argentina), Rubino (Uruguay), Cajamarca (Peru´ ) and Rı´o Chico (Catamarca, Argentina). The fluke eggs were incubated (258C) for a 12-h period in the presence of either ABZ or its sulphoxide metabolite (ABZ.SO) (5, 0.5 or 0.05 nmol/ml). Untreated eggs were incubated as a control. Incubated eggs (with or without drug present) were kept in darkness at 258C for 15 days. Afterwards, the trematode eggs were exposed to daylight over a 2-h period. Hatched and unhatched eggs were evaluated using an optical microscope, and the ovicidal activity was assessed for each fluke isolate. A very low ovicidal activity (#13.4%) was observed in the ABZ-resistant CEDIVE isolate for both ABZ and ABZ.SO. Conversely, in the INTA-Bariloche and Rı´o Chico isolates, which are suspected to be susceptible to ABZ, ovicidal activities $ 70.3% were observed after incubation with ABZ at the lowest concentration tested (0.05 nmol/ml). This finding correlates with that previously
Fil: Canevari, José. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Ceballos, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina
Fil: Romero, Jorge Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina
Fil: Olaechea, Fermín. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Ortiz, Pedro. Universidad Nacional de Cajamarca. Facultad de Ciencias Veterinarias; Perú
Fil: Cabrera, María. Universidad Nacional de Cajamarca. Facultad de Ciencias Veterinarias; Perú
Fil: Gayo, Valeria. Instituto DILAVE "Miguel C. Rubino"; Uruguay
Fil: Fairweather, Ian. The Queen’s University of Belfast. Medical Biological Centre. School of Biological Sciences. Parasite Therapeutics Research Group; Argentina
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Materia
Fasciola Hepatica
Albendazole
Resistance
Detection
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4702
Acceder
id CONICETDig_022a99a1e7fa697f2ac548f461c81f3c
oai_identifier_str oai:ri.conicet.gov.ar:11336/4702
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United KingdomCanevari, JoséCeballos, LauraSanabria, Rodrigo Eduardo FabrizioRomero, Jorge RobertoOlaechea, FermínOrtiz, PedroCabrera, MaríaGayo, ValeriaFairweather, IanLanusse, Carlos EdmundoAlvarez, Luis IgnacioFasciola HepaticaAlbendazoleResistanceDetectionhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The main goal of the current work was to develop and validate an in vitro fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke, Fasciola hepatica. Fluke eggs (200/ml, n ¼ 5) from six different isolates were used in the current experimental work. They were obtained from different geographical locations and named Cullompton (UK), CEDIVE (Chascomus, Argentina), INTA-Bariloche (Bariloche, Argentina), Rubino (Uruguay), Cajamarca (Peru´ ) and Rı´o Chico (Catamarca, Argentina). The fluke eggs were incubated (258C) for a 12-h period in the presence of either ABZ or its sulphoxide metabolite (ABZ.SO) (5, 0.5 or 0.05 nmol/ml). Untreated eggs were incubated as a control. Incubated eggs (with or without drug present) were kept in darkness at 258C for 15 days. Afterwards, the trematode eggs were exposed to daylight over a 2-h period. Hatched and unhatched eggs were evaluated using an optical microscope, and the ovicidal activity was assessed for each fluke isolate. A very low ovicidal activity (#13.4%) was observed in the ABZ-resistant CEDIVE isolate for both ABZ and ABZ.SO. Conversely, in the INTA-Bariloche and Rı´o Chico isolates, which are suspected to be susceptible to ABZ, ovicidal activities $ 70.3% were observed after incubation with ABZ at the lowest concentration tested (0.05 nmol/ml). This finding correlates with that previouslyFil: Canevari, José. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Ceballos, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; ArgentinaFil: Romero, Jorge Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; ArgentinaFil: Olaechea, Fermín. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Ortiz, Pedro. Universidad Nacional de Cajamarca. Facultad de Ciencias Veterinarias; PerúFil: Cabrera, María. Universidad Nacional de Cajamarca. Facultad de Ciencias Veterinarias; PerúFil: Gayo, Valeria. Instituto DILAVE "Miguel C. Rubino"; UruguayFil: Fairweather, Ian. The Queen’s University of Belfast. Medical Biological Centre. School of Biological Sciences. Parasite Therapeutics Research Group; ArgentinaFil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaCambridge University Press2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4702Canevari, José; Ceballos, Laura; Sanabria, Rodrigo Eduardo Fabrizio; Romero, Jorge Roberto; Olaechea, Fermín; et al.; Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom; Cambridge University Press; Journal Of Helminthology; 88; 3; 3-2013; 286-2920022-149Xenginfo:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9314027&fileId=S0022149X13000163info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1017/S0022149X13000163info:eu-repo/semantics/altIdentifier/issn/0022-149Xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:51:20Zoai:ri.conicet.gov.ar:11336/4702instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:51:21.175CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
title Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
spellingShingle Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
Canevari, José
Fasciola Hepatica
Albendazole
Resistance
Detection
title_short Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
title_full Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
title_fullStr Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
title_full_unstemmed Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
title_sort Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom
dc.creator.none.fl_str_mv Canevari, José
Ceballos, Laura
Sanabria, Rodrigo Eduardo Fabrizio
Romero, Jorge Roberto
Olaechea, Fermín
Ortiz, Pedro
Cabrera, María
Gayo, Valeria
Fairweather, Ian
Lanusse, Carlos Edmundo
Alvarez, Luis Ignacio
author Canevari, José
author_facet Canevari, José
Ceballos, Laura
Sanabria, Rodrigo Eduardo Fabrizio
Romero, Jorge Roberto
Olaechea, Fermín
Ortiz, Pedro
Cabrera, María
Gayo, Valeria
Fairweather, Ian
Lanusse, Carlos Edmundo
Alvarez, Luis Ignacio
author_role author
author2 Ceballos, Laura
Sanabria, Rodrigo Eduardo Fabrizio
Romero, Jorge Roberto
Olaechea, Fermín
Ortiz, Pedro
Cabrera, María
Gayo, Valeria
Fairweather, Ian
Lanusse, Carlos Edmundo
Alvarez, Luis Ignacio
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Fasciola Hepatica
Albendazole
Resistance
Detection
topic Fasciola Hepatica
Albendazole
Resistance
Detection
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The main goal of the current work was to develop and validate an in vitro fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke, Fasciola hepatica. Fluke eggs (200/ml, n ¼ 5) from six different isolates were used in the current experimental work. They were obtained from different geographical locations and named Cullompton (UK), CEDIVE (Chascomus, Argentina), INTA-Bariloche (Bariloche, Argentina), Rubino (Uruguay), Cajamarca (Peru´ ) and Rı´o Chico (Catamarca, Argentina). The fluke eggs were incubated (258C) for a 12-h period in the presence of either ABZ or its sulphoxide metabolite (ABZ.SO) (5, 0.5 or 0.05 nmol/ml). Untreated eggs were incubated as a control. Incubated eggs (with or without drug present) were kept in darkness at 258C for 15 days. Afterwards, the trematode eggs were exposed to daylight over a 2-h period. Hatched and unhatched eggs were evaluated using an optical microscope, and the ovicidal activity was assessed for each fluke isolate. A very low ovicidal activity (#13.4%) was observed in the ABZ-resistant CEDIVE isolate for both ABZ and ABZ.SO. Conversely, in the INTA-Bariloche and Rı´o Chico isolates, which are suspected to be susceptible to ABZ, ovicidal activities $ 70.3% were observed after incubation with ABZ at the lowest concentration tested (0.05 nmol/ml). This finding correlates with that previously
Fil: Canevari, José. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Ceballos, Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina
Fil: Romero, Jorge Roberto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Clinica. Centro de Diagnostico e Investigaciones Veterinarias; Argentina
Fil: Olaechea, Fermín. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Ortiz, Pedro. Universidad Nacional de Cajamarca. Facultad de Ciencias Veterinarias; Perú
Fil: Cabrera, María. Universidad Nacional de Cajamarca. Facultad de Ciencias Veterinarias; Perú
Fil: Gayo, Valeria. Instituto DILAVE "Miguel C. Rubino"; Uruguay
Fil: Fairweather, Ian. The Queen’s University of Belfast. Medical Biological Centre. School of Biological Sciences. Parasite Therapeutics Research Group; Argentina
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
description The main goal of the current work was to develop and validate an in vitro fluke egg hatch test, as a method for the detection of albendazole (ABZ) resistance in the liver fluke, Fasciola hepatica. Fluke eggs (200/ml, n ¼ 5) from six different isolates were used in the current experimental work. They were obtained from different geographical locations and named Cullompton (UK), CEDIVE (Chascomus, Argentina), INTA-Bariloche (Bariloche, Argentina), Rubino (Uruguay), Cajamarca (Peru´ ) and Rı´o Chico (Catamarca, Argentina). The fluke eggs were incubated (258C) for a 12-h period in the presence of either ABZ or its sulphoxide metabolite (ABZ.SO) (5, 0.5 or 0.05 nmol/ml). Untreated eggs were incubated as a control. Incubated eggs (with or without drug present) were kept in darkness at 258C for 15 days. Afterwards, the trematode eggs were exposed to daylight over a 2-h period. Hatched and unhatched eggs were evaluated using an optical microscope, and the ovicidal activity was assessed for each fluke isolate. A very low ovicidal activity (#13.4%) was observed in the ABZ-resistant CEDIVE isolate for both ABZ and ABZ.SO. Conversely, in the INTA-Bariloche and Rı´o Chico isolates, which are suspected to be susceptible to ABZ, ovicidal activities $ 70.3% were observed after incubation with ABZ at the lowest concentration tested (0.05 nmol/ml). This finding correlates with that previously
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4702
Canevari, José; Ceballos, Laura; Sanabria, Rodrigo Eduardo Fabrizio; Romero, Jorge Roberto; Olaechea, Fermín; et al.; Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom; Cambridge University Press; Journal Of Helminthology; 88; 3; 3-2013; 286-292
0022-149X
url http://hdl.handle.net/11336/4702
identifier_str_mv Canevari, José; Ceballos, Laura; Sanabria, Rodrigo Eduardo Fabrizio; Romero, Jorge Roberto; Olaechea, Fermín; et al.; Testing albendazole resistance in Fasciola hepatica: validation of an egg hatch test with isolates from South America and the United Kingdom; Cambridge University Press; Journal Of Helminthology; 88; 3; 3-2013; 286-292
0022-149X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9314027&fileId=S0022149X13000163
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1017/S0022149X13000163
info:eu-repo/semantics/altIdentifier/issn/0022-149X
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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