Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine

Autores
Alvarez, Luis Ignacio; Liron, Juan Pedro; Cantón, Candela; Castilla Gómez de Agüero, Verónica; Valderas García, Elora; Larroza, Marcela Patricia; Soler, Paula; Lanusse, Carlos Edmundo; Martinez Valladares, María
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Triclabendazole is the worldwide most used compound to control Fasciola hepatica and reports on drug resistance are abundant. Although the flukicidal use of albendazole (ABZ) is not as widespread, ABZ-resistance reports in liver flukes have increased in recent years. The work reported here describes a survey of the susceptibility/resistant status of different F. hepatica isolates to ABZ in two geographic regions of Spain and Argentine, using the egg hatch test (EHT). F. hepatica eggs were obtained from the gall bladder or faeces of natural infected cattle or sheep from different geographical areas of northern Spain (Castilla y León, Galicia and Asturias) and east (Litoral) and south (Patagonia) of Argentine. Fluke eggs were incubated at 25 ºC in darkness for a 12 h period with ABZ (0.5 nmol/mL). Untreated eggs served as control. After incubation, all eggs were gently washed to facilitate drug removal, and kept in darkness at 25 ºC for 15 days. After this period, eggs were exposed to light for 2 h to stimulate the hatching of miracidia. Hatched and unhatched (undeveloped) eggs were evaluated using an optical microscope and the ovicidal activity, expressed as a percentage, was calculated. A total of 42 (Spain) and 28 (Argentine) F. hepatica isolates were assessed for ABZ-resistance. After the EHT, 4 (9%) isolates from Spain results ABZ-resistant. The EHT could be successfully performed in 28 Argentinian isolates, resulting 75% of them resistant to ABZ. The high prevalence of ABZ-resistance in F. hepatica observed in Argentine can be explained by the widespread use of this flukicidal drug instead of triclabendazole. Oppositely, the low ABZ-resistance observed in the isolates collected from Spain may be related to the common use of a variety of alternative flukicidal drugs, such as closantel, nitroxynil and/or clorsulon.
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cantón, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Castilla Gómez de Agüero, Verónica. Universidad de León; España
Fil: Valderas García, Elora. Universidad de León; España
Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Martinez Valladares, María. Universidad de León; España
29th International Conference of the World Association for the Advancement of Veterinary Parasitology
Chennai
India
World Association for the Advancement of Veterinary Parasitology
Materia
FASCIOLA HEPATICA
RESISTANCE
ALBENDAZOLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/218717
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/218717
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and ArgentineAlvarez, Luis IgnacioLiron, Juan PedroCantón, CandelaCastilla Gómez de Agüero, VerónicaValderas García, EloraLarroza, Marcela PatriciaSoler, PaulaLanusse, Carlos EdmundoMartinez Valladares, MaríaFASCIOLA HEPATICARESISTANCEALBENDAZOLEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Triclabendazole is the worldwide most used compound to control Fasciola hepatica and reports on drug resistance are abundant. Although the flukicidal use of albendazole (ABZ) is not as widespread, ABZ-resistance reports in liver flukes have increased in recent years. The work reported here describes a survey of the susceptibility/resistant status of different F. hepatica isolates to ABZ in two geographic regions of Spain and Argentine, using the egg hatch test (EHT). F. hepatica eggs were obtained from the gall bladder or faeces of natural infected cattle or sheep from different geographical areas of northern Spain (Castilla y León, Galicia and Asturias) and east (Litoral) and south (Patagonia) of Argentine. Fluke eggs were incubated at 25 ºC in darkness for a 12 h period with ABZ (0.5 nmol/mL). Untreated eggs served as control. After incubation, all eggs were gently washed to facilitate drug removal, and kept in darkness at 25 ºC for 15 days. After this period, eggs were exposed to light for 2 h to stimulate the hatching of miracidia. Hatched and unhatched (undeveloped) eggs were evaluated using an optical microscope and the ovicidal activity, expressed as a percentage, was calculated. A total of 42 (Spain) and 28 (Argentine) F. hepatica isolates were assessed for ABZ-resistance. After the EHT, 4 (9%) isolates from Spain results ABZ-resistant. The EHT could be successfully performed in 28 Argentinian isolates, resulting 75% of them resistant to ABZ. The high prevalence of ABZ-resistance in F. hepatica observed in Argentine can be explained by the widespread use of this flukicidal drug instead of triclabendazole. Oppositely, the low ABZ-resistance observed in the isolates collected from Spain may be related to the common use of a variety of alternative flukicidal drugs, such as closantel, nitroxynil and/or clorsulon.Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Cantón, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Castilla Gómez de Agüero, Verónica. Universidad de León; EspañaFil: Valderas García, Elora. Universidad de León; EspañaFil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; ArgentinaFil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Martinez Valladares, María. Universidad de León; España29th International Conference of the World Association for the Advancement of Veterinary ParasitologyChennaiIndiaWorld Association for the Advancement of Veterinary ParasitologyWorld Association for the Advancement of Veterinary Parasitology2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/218717Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine; 29th International Conference of the World Association for the Advancement of Veterinary Parasitology; Chennai; India; 2023; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://waavp2023.com/final-abstracts/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:45:55Zoai:ri.conicet.gov.ar:11336/218717instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:45:55.279CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
title Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
spellingShingle Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
Alvarez, Luis Ignacio
FASCIOLA HEPATICA
RESISTANCE
ALBENDAZOLE
title_short Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
title_full Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
title_fullStr Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
title_full_unstemmed Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
title_sort Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine
dc.creator.none.fl_str_mv Alvarez, Luis Ignacio
Liron, Juan Pedro
Cantón, Candela
Castilla Gómez de Agüero, Verónica
Valderas García, Elora
Larroza, Marcela Patricia
Soler, Paula
Lanusse, Carlos Edmundo
Martinez Valladares, María
author Alvarez, Luis Ignacio
author_facet Alvarez, Luis Ignacio
Liron, Juan Pedro
Cantón, Candela
Castilla Gómez de Agüero, Verónica
Valderas García, Elora
Larroza, Marcela Patricia
Soler, Paula
Lanusse, Carlos Edmundo
Martinez Valladares, María
author_role author
author2 Liron, Juan Pedro
Cantón, Candela
Castilla Gómez de Agüero, Verónica
Valderas García, Elora
Larroza, Marcela Patricia
Soler, Paula
Lanusse, Carlos Edmundo
Martinez Valladares, María
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FASCIOLA HEPATICA
RESISTANCE
ALBENDAZOLE
topic FASCIOLA HEPATICA
RESISTANCE
ALBENDAZOLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Triclabendazole is the worldwide most used compound to control Fasciola hepatica and reports on drug resistance are abundant. Although the flukicidal use of albendazole (ABZ) is not as widespread, ABZ-resistance reports in liver flukes have increased in recent years. The work reported here describes a survey of the susceptibility/resistant status of different F. hepatica isolates to ABZ in two geographic regions of Spain and Argentine, using the egg hatch test (EHT). F. hepatica eggs were obtained from the gall bladder or faeces of natural infected cattle or sheep from different geographical areas of northern Spain (Castilla y León, Galicia and Asturias) and east (Litoral) and south (Patagonia) of Argentine. Fluke eggs were incubated at 25 ºC in darkness for a 12 h period with ABZ (0.5 nmol/mL). Untreated eggs served as control. After incubation, all eggs were gently washed to facilitate drug removal, and kept in darkness at 25 ºC for 15 days. After this period, eggs were exposed to light for 2 h to stimulate the hatching of miracidia. Hatched and unhatched (undeveloped) eggs were evaluated using an optical microscope and the ovicidal activity, expressed as a percentage, was calculated. A total of 42 (Spain) and 28 (Argentine) F. hepatica isolates were assessed for ABZ-resistance. After the EHT, 4 (9%) isolates from Spain results ABZ-resistant. The EHT could be successfully performed in 28 Argentinian isolates, resulting 75% of them resistant to ABZ. The high prevalence of ABZ-resistance in F. hepatica observed in Argentine can be explained by the widespread use of this flukicidal drug instead of triclabendazole. Oppositely, the low ABZ-resistance observed in the isolates collected from Spain may be related to the common use of a variety of alternative flukicidal drugs, such as closantel, nitroxynil and/or clorsulon.
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Liron, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Cantón, Candela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Castilla Gómez de Agüero, Verónica. Universidad de León; España
Fil: Valderas García, Elora. Universidad de León; España
Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Lanusse, Carlos Edmundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Martinez Valladares, María. Universidad de León; España
29th International Conference of the World Association for the Advancement of Veterinary Parasitology
Chennai
India
World Association for the Advancement of Veterinary Parasitology
description Triclabendazole is the worldwide most used compound to control Fasciola hepatica and reports on drug resistance are abundant. Although the flukicidal use of albendazole (ABZ) is not as widespread, ABZ-resistance reports in liver flukes have increased in recent years. The work reported here describes a survey of the susceptibility/resistant status of different F. hepatica isolates to ABZ in two geographic regions of Spain and Argentine, using the egg hatch test (EHT). F. hepatica eggs were obtained from the gall bladder or faeces of natural infected cattle or sheep from different geographical areas of northern Spain (Castilla y León, Galicia and Asturias) and east (Litoral) and south (Patagonia) of Argentine. Fluke eggs were incubated at 25 ºC in darkness for a 12 h period with ABZ (0.5 nmol/mL). Untreated eggs served as control. After incubation, all eggs were gently washed to facilitate drug removal, and kept in darkness at 25 ºC for 15 days. After this period, eggs were exposed to light for 2 h to stimulate the hatching of miracidia. Hatched and unhatched (undeveloped) eggs were evaluated using an optical microscope and the ovicidal activity, expressed as a percentage, was calculated. A total of 42 (Spain) and 28 (Argentine) F. hepatica isolates were assessed for ABZ-resistance. After the EHT, 4 (9%) isolates from Spain results ABZ-resistant. The EHT could be successfully performed in 28 Argentinian isolates, resulting 75% of them resistant to ABZ. The high prevalence of ABZ-resistance in F. hepatica observed in Argentine can be explained by the widespread use of this flukicidal drug instead of triclabendazole. Oppositely, the low ABZ-resistance observed in the isolates collected from Spain may be related to the common use of a variety of alternative flukicidal drugs, such as closantel, nitroxynil and/or clorsulon.
publishDate 2023
dc.date.none.fl_str_mv 2023
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info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/218717
Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine; 29th International Conference of the World Association for the Advancement of Veterinary Parasitology; Chennai; India; 2023; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/218717
identifier_str_mv Albendazole resistance in Fasciola hepatica: surveillance in different areas of Spain and Argentine; 29th International Conference of the World Association for the Advancement of Veterinary Parasitology; Chennai; India; 2023; 1-2
CONICET Digital
CONICET
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language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
publisher.none.fl_str_mv World Association for the Advancement of Veterinary Parasitology
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