Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test

Autores
Herschell Kelly, Emily; Ceballos, Laura; Maté, María Laura; Alvarez, Luis Ignacio; Hodgkinson, Jane
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The liver fluke Fasciola hepatica is a major cause of morbidity and mortality in ruminant livestock in many parts of the world and infection results in substantial economic losses to the sector. Intensive use of highly effective triclabendazole (>99% efficacy) to control the impact of F. hepatica infection has resulted in widespread resistance. Alternative anthelmintics, like the adulticide albendazole (ABZ) (≥80% efficacy), are often employed to reduce transmission. This study reports in vivo controlled efficacy tests (CET) and in vitro egg hatch test (EHT) evaluation of ABZ sensitivity for two F. hepatica isolates, one from Argentina (Ag) and one from Sweden (E). For each isolate CET: Sheep (n= 6) were infected with ~200 metacercariae, three were treated at 12 weeks post-infection with ABZ (7.5mg/kg), three were left untreated, total worm burden was calculated at necropsy 14 days post-treatment. A ≥80% reduction in CET = ABZ susceptible. EHT: Five x 200 eggs were exposed to ABZ (5, 0.5, 0.05 μM) or MeOH only. Ovicidal activity (%OA) at the discriminating dose (0.5μM) and statistical difference in egg development rate was calculated (pairwise t-test). Isolates were identified as: a) resistant = %OA ≤40%, no statistical difference, b) susceptible = %OA ≥70%, statistically significant difference, c) equivocal = between %OA >40% and <70%. For isolate Ag, CET = 57.65% reduction in worm burden in ABZ+ sheep compared to untreated controls (ABZ- mean = 85 ±21.8 SD, ABZ+ mean = 36 ± 7.94 SD, p = 0.047), indicating ABZ resistance. In EHT at the 0.5μM discriminating dose, % OA = 45.6% ± 1.96 SD and 41.2% ± 1.5 SD development rate (p = 0.01) indicating they fell in the equivocal range. For isolate E CET = 89.23% reduction in worm burden (ABZ- mean = 97.5 ± 6.36 SD, ABZ+ mean = 10.5 ± 9.19 SD, p = 0.012), indicating ABZ susceptibility. In EHT %OA = 74.0% ± 3.5 SD at 0.5 μM, (p < 0.001) and development rate of 19.6% ± 3.5 SD (p< 0.05), indicating ABZ susceptibility. The EHT can reliably screen F. hepatica populations for their susceptibility to ABZ, but it may only detect ABZ resistance in highly resistant populations. However, it is a valuable screening method for phenotyping parasites for molecular studies on signatures of ABZ resistance.
Fil: Herschell Kelly, Emily. University of Liverpool; Reino Unido
Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Maté, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Hodgkinson, Jane. University of Liverpool; Reino Unido
30th Conference ot the Wolrd Association for the Advancement of Veterinary Parasitology
Curitiba
Brasil
Wolrd Association for the Advancement of Veterinary Parasitology
Materia
FASCIOLA HEPATICA
RESISTANCE
EHT
SHEEP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/279420
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch testHerschell Kelly, EmilyCeballos, LauraMaté, María LauraAlvarez, Luis IgnacioHodgkinson, JaneFASCIOLA HEPATICARESISTANCEEHTSHEEPhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The liver fluke Fasciola hepatica is a major cause of morbidity and mortality in ruminant livestock in many parts of the world and infection results in substantial economic losses to the sector. Intensive use of highly effective triclabendazole (>99% efficacy) to control the impact of F. hepatica infection has resulted in widespread resistance. Alternative anthelmintics, like the adulticide albendazole (ABZ) (≥80% efficacy), are often employed to reduce transmission. This study reports in vivo controlled efficacy tests (CET) and in vitro egg hatch test (EHT) evaluation of ABZ sensitivity for two F. hepatica isolates, one from Argentina (Ag) and one from Sweden (E). For each isolate CET: Sheep (n= 6) were infected with ~200 metacercariae, three were treated at 12 weeks post-infection with ABZ (7.5mg/kg), three were left untreated, total worm burden was calculated at necropsy 14 days post-treatment. A ≥80% reduction in CET = ABZ susceptible. EHT: Five x 200 eggs were exposed to ABZ (5, 0.5, 0.05 μM) or MeOH only. Ovicidal activity (%OA) at the discriminating dose (0.5μM) and statistical difference in egg development rate was calculated (pairwise t-test). Isolates were identified as: a) resistant = %OA ≤40%, no statistical difference, b) susceptible = %OA ≥70%, statistically significant difference, c) equivocal = between %OA >40% and <70%. For isolate Ag, CET = 57.65% reduction in worm burden in ABZ+ sheep compared to untreated controls (ABZ- mean = 85 ±21.8 SD, ABZ+ mean = 36 ± 7.94 SD, p = 0.047), indicating ABZ resistance. In EHT at the 0.5μM discriminating dose, % OA = 45.6% ± 1.96 SD and 41.2% ± 1.5 SD development rate (p = 0.01) indicating they fell in the equivocal range. For isolate E CET = 89.23% reduction in worm burden (ABZ- mean = 97.5 ± 6.36 SD, ABZ+ mean = 10.5 ± 9.19 SD, p = 0.012), indicating ABZ susceptibility. In EHT %OA = 74.0% ± 3.5 SD at 0.5 μM, (p < 0.001) and development rate of 19.6% ± 3.5 SD (p< 0.05), indicating ABZ susceptibility. The EHT can reliably screen F. hepatica populations for their susceptibility to ABZ, but it may only detect ABZ resistance in highly resistant populations. However, it is a valuable screening method for phenotyping parasites for molecular studies on signatures of ABZ resistance.Fil: Herschell Kelly, Emily. University of Liverpool; Reino UnidoFil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Maté, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; ArgentinaFil: Hodgkinson, Jane. University of Liverpool; Reino Unido30th Conference ot the Wolrd Association for the Advancement of Veterinary ParasitologyCuritibaBrasilWolrd Association for the Advancement of Veterinary ParasitologyWolrd Association for the Advancement of Veterinary Parasitology2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectConferenciaBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/279420Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test; 30th Conference ot the Wolrd Association for the Advancement of Veterinary Parasitology; Curitiba; Brasil; 2025; 15-16CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://waavp2025.com/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:30:56Zoai:ri.conicet.gov.ar:11336/279420instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:30:56.578CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
title Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
spellingShingle Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
Herschell Kelly, Emily
FASCIOLA HEPATICA
RESISTANCE
EHT
SHEEP
title_short Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
title_full Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
title_fullStr Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
title_full_unstemmed Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
title_sort Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test
dc.creator.none.fl_str_mv Herschell Kelly, Emily
Ceballos, Laura
Maté, María Laura
Alvarez, Luis Ignacio
Hodgkinson, Jane
author Herschell Kelly, Emily
author_facet Herschell Kelly, Emily
Ceballos, Laura
Maté, María Laura
Alvarez, Luis Ignacio
Hodgkinson, Jane
author_role author
author2 Ceballos, Laura
Maté, María Laura
Alvarez, Luis Ignacio
Hodgkinson, Jane
author2_role author
author
author
author
dc.subject.none.fl_str_mv FASCIOLA HEPATICA
RESISTANCE
EHT
SHEEP
topic FASCIOLA HEPATICA
RESISTANCE
EHT
SHEEP
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The liver fluke Fasciola hepatica is a major cause of morbidity and mortality in ruminant livestock in many parts of the world and infection results in substantial economic losses to the sector. Intensive use of highly effective triclabendazole (>99% efficacy) to control the impact of F. hepatica infection has resulted in widespread resistance. Alternative anthelmintics, like the adulticide albendazole (ABZ) (≥80% efficacy), are often employed to reduce transmission. This study reports in vivo controlled efficacy tests (CET) and in vitro egg hatch test (EHT) evaluation of ABZ sensitivity for two F. hepatica isolates, one from Argentina (Ag) and one from Sweden (E). For each isolate CET: Sheep (n= 6) were infected with ~200 metacercariae, three were treated at 12 weeks post-infection with ABZ (7.5mg/kg), three were left untreated, total worm burden was calculated at necropsy 14 days post-treatment. A ≥80% reduction in CET = ABZ susceptible. EHT: Five x 200 eggs were exposed to ABZ (5, 0.5, 0.05 μM) or MeOH only. Ovicidal activity (%OA) at the discriminating dose (0.5μM) and statistical difference in egg development rate was calculated (pairwise t-test). Isolates were identified as: a) resistant = %OA ≤40%, no statistical difference, b) susceptible = %OA ≥70%, statistically significant difference, c) equivocal = between %OA >40% and <70%. For isolate Ag, CET = 57.65% reduction in worm burden in ABZ+ sheep compared to untreated controls (ABZ- mean = 85 ±21.8 SD, ABZ+ mean = 36 ± 7.94 SD, p = 0.047), indicating ABZ resistance. In EHT at the 0.5μM discriminating dose, % OA = 45.6% ± 1.96 SD and 41.2% ± 1.5 SD development rate (p = 0.01) indicating they fell in the equivocal range. For isolate E CET = 89.23% reduction in worm burden (ABZ- mean = 97.5 ± 6.36 SD, ABZ+ mean = 10.5 ± 9.19 SD, p = 0.012), indicating ABZ susceptibility. In EHT %OA = 74.0% ± 3.5 SD at 0.5 μM, (p < 0.001) and development rate of 19.6% ± 3.5 SD (p< 0.05), indicating ABZ susceptibility. The EHT can reliably screen F. hepatica populations for their susceptibility to ABZ, but it may only detect ABZ resistance in highly resistant populations. However, it is a valuable screening method for phenotyping parasites for molecular studies on signatures of ABZ resistance.
Fil: Herschell Kelly, Emily. University of Liverpool; Reino Unido
Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Maté, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Hodgkinson, Jane. University of Liverpool; Reino Unido
30th Conference ot the Wolrd Association for the Advancement of Veterinary Parasitology
Curitiba
Brasil
Wolrd Association for the Advancement of Veterinary Parasitology
description The liver fluke Fasciola hepatica is a major cause of morbidity and mortality in ruminant livestock in many parts of the world and infection results in substantial economic losses to the sector. Intensive use of highly effective triclabendazole (>99% efficacy) to control the impact of F. hepatica infection has resulted in widespread resistance. Alternative anthelmintics, like the adulticide albendazole (ABZ) (≥80% efficacy), are often employed to reduce transmission. This study reports in vivo controlled efficacy tests (CET) and in vitro egg hatch test (EHT) evaluation of ABZ sensitivity for two F. hepatica isolates, one from Argentina (Ag) and one from Sweden (E). For each isolate CET: Sheep (n= 6) were infected with ~200 metacercariae, three were treated at 12 weeks post-infection with ABZ (7.5mg/kg), three were left untreated, total worm burden was calculated at necropsy 14 days post-treatment. A ≥80% reduction in CET = ABZ susceptible. EHT: Five x 200 eggs were exposed to ABZ (5, 0.5, 0.05 μM) or MeOH only. Ovicidal activity (%OA) at the discriminating dose (0.5μM) and statistical difference in egg development rate was calculated (pairwise t-test). Isolates were identified as: a) resistant = %OA ≤40%, no statistical difference, b) susceptible = %OA ≥70%, statistically significant difference, c) equivocal = between %OA >40% and <70%. For isolate Ag, CET = 57.65% reduction in worm burden in ABZ+ sheep compared to untreated controls (ABZ- mean = 85 ±21.8 SD, ABZ+ mean = 36 ± 7.94 SD, p = 0.047), indicating ABZ resistance. In EHT at the 0.5μM discriminating dose, % OA = 45.6% ± 1.96 SD and 41.2% ± 1.5 SD development rate (p = 0.01) indicating they fell in the equivocal range. For isolate E CET = 89.23% reduction in worm burden (ABZ- mean = 97.5 ± 6.36 SD, ABZ+ mean = 10.5 ± 9.19 SD, p = 0.012), indicating ABZ susceptibility. In EHT %OA = 74.0% ± 3.5 SD at 0.5 μM, (p < 0.001) and development rate of 19.6% ± 3.5 SD (p< 0.05), indicating ABZ susceptibility. The EHT can reliably screen F. hepatica populations for their susceptibility to ABZ, but it may only detect ABZ resistance in highly resistant populations. However, it is a valuable screening method for phenotyping parasites for molecular studies on signatures of ABZ resistance.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Conferencia
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/279420
Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test; 30th Conference ot the Wolrd Association for the Advancement of Veterinary Parasitology; Curitiba; Brasil; 2025; 15-16
CONICET Digital
CONICET
url http://hdl.handle.net/11336/279420
identifier_str_mv Phenotyping Fasciola hepatica populations for albendazole sensitivity by in vitro egg hatch test; 30th Conference ot the Wolrd Association for the Advancement of Veterinary Parasitology; Curitiba; Brasil; 2025; 15-16
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://waavp2025.com/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Wolrd Association for the Advancement of Veterinary Parasitology
publisher.none.fl_str_mv Wolrd Association for the Advancement of Veterinary Parasitology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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