Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
- Autores
- Pontoriero, Andrea; Baumeister, Elsa; Campos, Ana; Savy, Vilma L.
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Savy, Vilma L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Objective. To describe the virological characteristics of the influenza strains circulating in Argentina in 2005–2008 and to assess the prevalence of antiviral resistance. Methods. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin–Darbycanine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and yrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene. Results. Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir. Conclusions. This study describes a high incidence of amantadineresistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern. - Fuente
- Pan American Journal of Public Health / Revista Panamericana de Salud Pública, 2011, 30(6), 634–640.
- Materia
-
Virus de la Influenza A
Resistencia a Medicamentos
Antivirales
Argentina - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/290
Ver los metadatos del registro completo
id |
SGCANLIS_7dbb4948154eaee0d32b9213c11a3cc9 |
---|---|
oai_identifier_str |
oai:sgc.anlis.gob.ar:123456789/290 |
network_acronym_str |
SGCANLIS |
repository_id_str |
a |
network_name_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
spelling |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008Vigilancia virológica y resistencia a los antivíricos del virus de la gripe humana en la Argentina, 2005–2008Pontoriero, AndreaBaumeister, ElsaCampos, AnaSavy, Vilma L.Virus de la Influenza AResistencia a MedicamentosAntiviralesArgentinaFil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Savy, Vilma L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Objective. To describe the virological characteristics of the influenza strains circulating in Argentina in 2005–2008 and to assess the prevalence of antiviral resistance. Methods. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin–Darbycanine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and yrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene. Results. Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir. Conclusions. This study describes a high incidence of amantadineresistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.World Health Organization2011info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1020-4989http://sgc.anlis.gob.ar/handle/123456789/290http://www.scielosp.org/pdf/rpsp/v30n6/a23v30n6.pdfPan American Journal of Public Health / Revista Panamericana de Salud Pública, 2011, 30(6), 634–640.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISRevista panamericana de salud publica = Pan American journal of public healthARG2005-2008enginfo:eu-repo/semantics/openAccess2025-09-29T14:29:57Zoai:sgc.anlis.gob.ar:123456789/290Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:29:57.725Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 Vigilancia virológica y resistencia a los antivíricos del virus de la gripe humana en la Argentina, 2005–2008 |
title |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
spellingShingle |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 Pontoriero, Andrea Virus de la Influenza A Resistencia a Medicamentos Antivirales Argentina |
title_short |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
title_full |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
title_fullStr |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
title_full_unstemmed |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
title_sort |
Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
dc.creator.none.fl_str_mv |
Pontoriero, Andrea Baumeister, Elsa Campos, Ana Savy, Vilma L. |
author |
Pontoriero, Andrea |
author_facet |
Pontoriero, Andrea Baumeister, Elsa Campos, Ana Savy, Vilma L. |
author_role |
author |
author2 |
Baumeister, Elsa Campos, Ana Savy, Vilma L. |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
Virus de la Influenza A Resistencia a Medicamentos Antivirales Argentina |
topic |
Virus de la Influenza A Resistencia a Medicamentos Antivirales Argentina |
dc.description.none.fl_txt_mv |
Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Fil: Savy, Vilma L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. Objective. To describe the virological characteristics of the influenza strains circulating in Argentina in 2005–2008 and to assess the prevalence of antiviral resistance. Methods. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin–Darbycanine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and yrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene. Results. Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir. Conclusions. This study describes a high incidence of amantadineresistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern. |
description |
Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
1020-4989 http://sgc.anlis.gob.ar/handle/123456789/290 http://www.scielosp.org/pdf/rpsp/v30n6/a23v30n6.pdf |
identifier_str_mv |
1020-4989 |
url |
http://sgc.anlis.gob.ar/handle/123456789/290 http://www.scielosp.org/pdf/rpsp/v30n6/a23v30n6.pdf |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista panamericana de salud publica = Pan American journal of public health |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
ARG 2005-2008 |
dc.publisher.none.fl_str_mv |
World Health Organization |
publisher.none.fl_str_mv |
World Health Organization |
dc.source.none.fl_str_mv |
Pan American Journal of Public Health / Revista Panamericana de Salud Pública, 2011, 30(6), 634–640. reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
reponame_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
collection |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
instname_str |
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
institution |
ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
_version_ |
1844621851362328576 |
score |
12.559606 |