Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008

Autores
Pontoriero, Andrea; Baumeister, Elsa; Campos, Ana; Savy, Vilma L.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Savy, Vilma L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Objective. To describe the virological characteristics of the influenza strains circulating in Argentina in 2005–2008 and to assess the prevalence of antiviral resistance. Methods. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin–Darbycanine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and yrosequenc­ing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance mar­kers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene. Results. Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir. Conclusions. This study describes a high incidence of amantadine­resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.
Fuente
Pan American Journal of Public Health / Revista Panamericana de Salud Pública, 2011, 30(6), 634–640.
Materia
Virus de la Influenza A
Resistencia a Medicamentos
Antivirales
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:Publications/123456789/290

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network_acronym_str SGCANLIS
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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008Vigilancia virológica y resistencia a los antivíricos del virus de la gripe humana en la Argentina, 2005–2008Pontoriero, AndreaBaumeister, ElsaCampos, AnaSavy, Vilma L.Virus de la Influenza AResistencia a MedicamentosAntiviralesArgentinaFil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Fil: Savy, Vilma L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.Objective. To describe the virological characteristics of the influenza strains circulating in Argentina in 2005–2008 and to assess the prevalence of antiviral resistance. Methods. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin–Darbycanine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and yrosequenc­ing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance mar­kers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene. Results. Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir. Conclusions. This study describes a high incidence of amantadine­resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.World Health Organization2011info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf1020-4989http://sgc.anlis.gob.ar/handle/123456789/290http://www.scielosp.org/pdf/rpsp/v30n6/a23v30n6.pdfPan American Journal of Public Health / Revista Panamericana de Salud Pública, 2011, 30(6), 634–640.reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISRevista panamericana de salud publica = Pan American journal of public healthARG2005-2008enginfo:eu-repo/semantics/openAccess2025-09-29T14:29:57Zoai:sgc.anlis.gob.ar:Publications/123456789/290Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-29 14:29:57.729Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
Vigilancia virológica y resistencia a los antivíricos del virus de la gripe humana en la Argentina, 2005–2008
title Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
spellingShingle Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
Pontoriero, Andrea
Virus de la Influenza A
Resistencia a Medicamentos
Antivirales
Argentina
title_short Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
title_full Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
title_fullStr Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
title_full_unstemmed Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
title_sort Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
dc.creator.none.fl_str_mv Pontoriero, Andrea
Baumeister, Elsa
Campos, Ana
Savy, Vilma L.
author Pontoriero, Andrea
author_facet Pontoriero, Andrea
Baumeister, Elsa
Campos, Ana
Savy, Vilma L.
author_role author
author2 Baumeister, Elsa
Campos, Ana
Savy, Vilma L.
author2_role author
author
author
dc.subject.none.fl_str_mv Virus de la Influenza A
Resistencia a Medicamentos
Antivirales
Argentina
topic Virus de la Influenza A
Resistencia a Medicamentos
Antivirales
Argentina
dc.description.none.fl_txt_mv Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Baumeister, Elsa. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Campos, Ana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Fil: Savy, Vilma L. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
Objective. To describe the virological characteristics of the influenza strains circulating in Argentina in 2005–2008 and to assess the prevalence of antiviral resistance. Methods. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin–Darbycanine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and yrosequenc­ing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance mar­kers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene. Results. Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir. Conclusions. This study describes a high incidence of amantadine­resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.
description Fil: Pontoriero, Andrea. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Virología; Argentina.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 1020-4989
http://sgc.anlis.gob.ar/handle/123456789/290
http://www.scielosp.org/pdf/rpsp/v30n6/a23v30n6.pdf
identifier_str_mv 1020-4989
url http://sgc.anlis.gob.ar/handle/123456789/290
http://www.scielosp.org/pdf/rpsp/v30n6/a23v30n6.pdf
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista panamericana de salud publica = Pan American journal of public health
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv ARG
2005-2008
dc.publisher.none.fl_str_mv World Health Organization
publisher.none.fl_str_mv World Health Organization
dc.source.none.fl_str_mv Pan American Journal of Public Health / Revista Panamericana de Salud Pública, 2011, 30(6), 634–640.
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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