Assessment of CD8(+) T cell differentiation in Trypanosoma cruzi-infected children

• Autores: Albareda, María Cecilia; Olivera, Gabriela C; De Rissio, Ana María; Postan, Miriam
• Año de publicación: 2010
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: The American journal of tropical medicine and hygiene, 2010, 82(5), 861-864.
• Materia: Enfermedad de Chagas; Linfocitos T CD8-positivos; Diferenciación Celular; Citocinas; Regulación de la Expresión Génica
• Institución: Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"

Fil: Albareda, María Cecilia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Postan, Miriam. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: De Rissio, Ana María. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
Fil: Olivera, Gabriela C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Parasitología; Argentina.
We previously reported that the T cell compartment in chronically Trypanosoma cruzi-infected adult subjects display functional and phenotypic signs of immune senescence. This study aimed to investigate the differentiation and the senescent profile of the overall CD8(+) T cell compartment in T. cruzi-infected children at the early stage of the disease. We found a lower percentage of naive (CD27(+)CD28(+)CD45RA(+)) and early antigen-experienced (CD45RA(-)CD27(+)CD28(+)), and higher percentages of late differentiated antigen-experienced (CD45RA(-)CD27(-)CD28(-)) CD8(+) T cells in T. cruzi-infected children as compared with age-matched uninfected controls. The expression of the interleukin (IL)-7R is also decreased on naive and on antigen-experienced total CD8(+) T cells with various degrees of differentiation. Conversely, the expression of HLA-DR, caspase-3, and CD57 did not vary on the total CD8(+) T cell compartment. These findings suggest that the duration of the infection is relevant in the process of immune senescent that this parasite can induce.