The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development
- Autores
- Naipauer, Julián; Gatelli, Albana; Degese, María Sol; Slomiansky, Victoria; Wertheimer Hermitte, Eva Victoria; Lamarre, Jonathan; Castilla, Lucio; Abba, Martín Carlos; Kordon, Edith Claudia; Coso, Omar Adrian
- Año de publicación
- 2013
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a new regulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function.
Centro de Investigaciones Inmunológicas Básicas y Aplicadas - Materia
-
Bioquímica
Alternative polyadenylation
Gene expression regulation
HuR
Itgbl
Mammary gland
mRNA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/102270
Ver los metadatos del registro completo
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The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland developmentNaipauer, JuliánGatelli, AlbanaDegese, María SolSlomiansky, VictoriaWertheimer Hermitte, Eva VictoriaLamarre, JonathanCastilla, LucioAbba, Martín CarlosKordon, Edith ClaudiaCoso, Omar AdrianBioquímicaAlternative polyadenylationGene expression regulationHuRItgblMammary glandmRNAIntegrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a new regulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function.Centro de Investigaciones Inmunológicas Básicas y Aplicadas2013-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf345-357http://sedici.unlp.edu.ar/handle/10915/102270enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/13692info:eu-repo/semantics/altIdentifier/url/http://www.biochemj.org/content/454/2/345info:eu-repo/semantics/altIdentifier/issn/1470-8728info:eu-repo/semantics/altIdentifier/doi/10.1042/BJ20130062info:eu-repo/semantics/altIdentifier/hdl/11336/13692info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:56Zoai:sedici.unlp.edu.ar:10915/102270Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:56.696SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| title |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| spellingShingle |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development Naipauer, Julián Bioquímica Alternative polyadenylation Gene expression regulation HuR Itgbl Mammary gland mRNA |
| title_short |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| title_full |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| title_fullStr |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| title_full_unstemmed |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| title_sort |
The use of alternative polyadenylation sites renders integrin β1 (Itgb1) mRNA isoforms with differential stability during mammary gland development |
| dc.creator.none.fl_str_mv |
Naipauer, Julián Gatelli, Albana Degese, María Sol Slomiansky, Victoria Wertheimer Hermitte, Eva Victoria Lamarre, Jonathan Castilla, Lucio Abba, Martín Carlos Kordon, Edith Claudia Coso, Omar Adrian |
| author |
Naipauer, Julián |
| author_facet |
Naipauer, Julián Gatelli, Albana Degese, María Sol Slomiansky, Victoria Wertheimer Hermitte, Eva Victoria Lamarre, Jonathan Castilla, Lucio Abba, Martín Carlos Kordon, Edith Claudia Coso, Omar Adrian |
| author_role |
author |
| author2 |
Gatelli, Albana Degese, María Sol Slomiansky, Victoria Wertheimer Hermitte, Eva Victoria Lamarre, Jonathan Castilla, Lucio Abba, Martín Carlos Kordon, Edith Claudia Coso, Omar Adrian |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bioquímica Alternative polyadenylation Gene expression regulation HuR Itgbl Mammary gland mRNA |
| topic |
Bioquímica Alternative polyadenylation Gene expression regulation HuR Itgbl Mammary gland mRNA |
| dc.description.none.fl_txt_mv |
Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a new regulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function. Centro de Investigaciones Inmunológicas Básicas y Aplicadas |
| description |
Integrins are heterodimeric cell-surface adhesion receptors that play a critical role in tissue development. Characterization of the full-length mRNA encoding the β1 subunit (Itgb1) revealed an alternative functional cleavage and polyadenylation site that yields a new Itgb1 mRNA isoform 578 bp shorter than that previously reported. Using a variety of experimental and bioinformatic approaches, we found that the two Itgb1 isoforms are expressed at different levels in a variety of mouse tissues, including the mammary gland, where they are differentially regulated at successive developmental stages. The longer mRNA species is prevelant during lactation, whereas the shorter is induced after weaning. In 3D cultures, where expression of integrin β1 protein is required for normal formation of acini, experimental blockade of the longer isoform induced enhanced expression of the shorter species which allowed normal morphological mammary differentiation. The short isoform lacks AU-rich motifs and miRNA target sequences that are potentially implicated in the regulation of mRNA stability and translation efficiency. We further determined that the AU-binding protein HuR appears to selectively stabilize the longer isoform in the mammary gland. In summary, the results of the present study identify a new regulatory instance involved in the fine-tuning of Itgb1 expression during mammary gland development and function. |
| publishDate |
2013 |
| dc.date.none.fl_str_mv |
2013-09 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/102270 |
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eng |
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eng |
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