First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA

Autores
Beltrame, María Ornela; Pruzzo, César Iván; Sanabria, Rodrigo Eduardo Fabrizio; Pérez, Alberto E.; Mora, Matías Sebastián
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/125996
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/125996
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNABeltrame, María OrnelaPruzzo, César IvánSanabria, Rodrigo Eduardo FabrizioPérez, Alberto E.Mora, Matías SebastiánCiencias VeterinariasaDNADEERFasciola hepaticaPalaeoparasitologyIt is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.Facultad de Ciencias Veterinarias2019-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf371-375http://sedici.unlp.edu.ar/handle/10915/125996enginfo:eu-repo/semantics/altIdentifier/issn/1469-8161info:eu-repo/semantics/altIdentifier/issn/0031-1820info:eu-repo/semantics/altIdentifier/pmid/31789141info:eu-repo/semantics/altIdentifier/doi/10.1017/s0031182019001719info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:18Zoai:sedici.unlp.edu.ar:10915/125996Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:19.055SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
spellingShingle First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
Beltrame, María Ornela
Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
title_short First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_full First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_fullStr First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_full_unstemmed First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_sort First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
dc.creator.none.fl_str_mv Beltrame, María Ornela
Pruzzo, César Iván
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto E.
Mora, Matías Sebastián
author Beltrame, María Ornela
author_facet Beltrame, María Ornela
Pruzzo, César Iván
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto E.
Mora, Matías Sebastián
author_role author
author2 Pruzzo, César Iván
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto E.
Mora, Matías Sebastián
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
topic Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
dc.description.none.fl_txt_mv It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
Facultad de Ciencias Veterinarias
description It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/125996
url http://sedici.unlp.edu.ar/handle/10915/125996
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1469-8161
info:eu-repo/semantics/altIdentifier/issn/0031-1820
info:eu-repo/semantics/altIdentifier/pmid/31789141
info:eu-repo/semantics/altIdentifier/doi/10.1017/s0031182019001719
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
371-375
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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