First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA

Autores
Beltrame, Maria Ornela; Pruzzo, César; Sanabria, Rodrigo Eduardo Fabrizio; Pérez, Alberto; Mora, Matias Sebastian
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
Fil: Beltrame, Maria Ornela. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Fil: Pruzzo, César. Universidad Nacional de La Plata; Argentina
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: Pérez, Alberto. Universidad Católica de Temuco; Chile
Fil: Mora, Matias Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
Materia
PALEOPARASITOLOGY
FASCIOLA HEPATICA
aDNA
DEER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/121956
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/121956
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNABeltrame, Maria OrnelaPruzzo, CésarSanabria, Rodrigo Eduardo FabrizioPérez, AlbertoMora, Matias SebastianPALEOPARASITOLOGYFASCIOLA HEPATICAaDNADEERhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.Fil: Beltrame, Maria Ornela. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; ArgentinaFil: Pruzzo, César. Universidad Nacional de La Plata; ArgentinaFil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Pérez, Alberto. Universidad Católica de Temuco; ChileFil: Mora, Matias Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; ArgentinaCambridge University Press2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/121956Beltrame, Maria Ornela; Pruzzo, César; Sanabria, Rodrigo Eduardo Fabrizio; Pérez, Alberto; Mora, Matias Sebastian; First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA; Cambridge University Press; Parasitology; 147; 3; 12-2019; 371-3750031-1820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/abs/first-report-of-prehispanic-fasciola-hepatica-from-south-america-revealed-by-ancient-dna/7F10A4E336F65401A7A9DA427D549B71info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182019001719info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:33:21Zoai:ri.conicet.gov.ar:11336/121956instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:33:21.969CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
spellingShingle First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
Beltrame, Maria Ornela
PALEOPARASITOLOGY
FASCIOLA HEPATICA
aDNA
DEER
title_short First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_full First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_fullStr First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_full_unstemmed First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_sort First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
dc.creator.none.fl_str_mv Beltrame, Maria Ornela
Pruzzo, César
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto
Mora, Matias Sebastian
author Beltrame, Maria Ornela
author_facet Beltrame, Maria Ornela
Pruzzo, César
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto
Mora, Matias Sebastian
author_role author
author2 Pruzzo, César
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto
Mora, Matias Sebastian
author2_role author
author
author
author
dc.subject.none.fl_str_mv PALEOPARASITOLOGY
FASCIOLA HEPATICA
aDNA
DEER
topic PALEOPARASITOLOGY
FASCIOLA HEPATICA
aDNA
DEER
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
Fil: Beltrame, Maria Ornela. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones En Sanidad Produccion y Ambiente. - Comision de Investigaciones Cientificas de la Provincia de Buenos Aires. Instituto de Investigaciones En Sanidad Produccion y Ambiente.; Argentina
Fil: Pruzzo, César. Universidad Nacional de La Plata; Argentina
Fil: Sanabria, Rodrigo Eduardo Fabrizio. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: Pérez, Alberto. Universidad Católica de Temuco; Chile
Fil: Mora, Matias Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Marinas y Costeras. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Marinas y Costeras; Argentina
description It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/121956
Beltrame, Maria Ornela; Pruzzo, César; Sanabria, Rodrigo Eduardo Fabrizio; Pérez, Alberto; Mora, Matias Sebastian; First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA; Cambridge University Press; Parasitology; 147; 3; 12-2019; 371-375
0031-1820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/121956
identifier_str_mv Beltrame, Maria Ornela; Pruzzo, César; Sanabria, Rodrigo Eduardo Fabrizio; Pérez, Alberto; Mora, Matias Sebastian; First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA; Cambridge University Press; Parasitology; 147; 3; 12-2019; 371-375
0031-1820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/abs/first-report-of-prehispanic-fasciola-hepatica-from-south-america-revealed-by-ancient-dna/7F10A4E336F65401A7A9DA427D549B71
info:eu-repo/semantics/altIdentifier/doi/10.1017/S0031182019001719
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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