Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants

Autores
Reigosa, Miguel Antonio; Labarta, Verónica; Molinari, Gabriela; Bernales, D.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several types of materials are frequently used for dental prostheses in dental medicine. Different treatments with titanium are the most used. The aim of the present study was to analyze by means of cytotoxicity and cytocompatibility techniques the capacity of dental implants to integrate to the bone tissue. Cultures of UMR 106 cell line derived from an osteosarcoma were used for bioassays mainly because they show many of the properties of osteoblasts. Dental implant samples provided by B&W company were compared with others of recognized trademarks. The first ones contain ASTM titanium (8348 GR2) with acid printing. Cytotoxicity was analyzed by means of lysosome activity, using the neutral red technique and alkaline phosphatase enzyme activity. Cell variability was determined by means of the acridine ethidium-orange bromide technique. One-way ANOVA and Bonferroni and Duncan post-ANOVA tests were used for the statistical analysis. The assays did not show significant differences among the dental implants analyzed. Our findings show that the dental prostheses studied present high biocompatibility, quantified by the bioassays performed. The techniques employed revealed that they can be a useful tool for the analysis of other materials for dental medicine use.
Instituto Multidisciplinario de Biología Celular
Materia
Ciencias Exactas
Biología
cytotoxicity
cytocompatibility
genotoxicity
dental implants
osteoblasts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127391
Acceder
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network_name_str SEDICI (UNLP)
spelling Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implantsReigosa, Miguel AntonioLabarta, VerónicaMolinari, GabrielaBernales, D.Ciencias ExactasBiologíacytotoxicitycytocompatibilitygenotoxicitydental implantsosteoblastsSeveral types of materials are frequently used for dental prostheses in dental medicine. Different treatments with titanium are the most used. The aim of the present study was to analyze by means of cytotoxicity and cytocompatibility techniques the capacity of dental implants to integrate to the bone tissue. Cultures of UMR 106 cell line derived from an osteosarcoma were used for bioassays mainly because they show many of the properties of osteoblasts. Dental implant samples provided by B&W company were compared with others of recognized trademarks. The first ones contain ASTM titanium (8348 GR2) with acid printing. Cytotoxicity was analyzed by means of lysosome activity, using the neutral red technique and alkaline phosphatase enzyme activity. Cell variability was determined by means of the acridine ethidium-orange bromide technique. One-way ANOVA and Bonferroni and Duncan post-ANOVA tests were used for the statistical analysis. The assays did not show significant differences among the dental implants analyzed. Our findings show that the dental prostheses studied present high biocompatibility, quantified by the bioassays performed. The techniques employed revealed that they can be a useful tool for the analysis of other materials for dental medicine use.Instituto Multidisciplinario de Biología Celular2007-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/127391enginfo:eu-repo/semantics/altIdentifier/issn/1742-6596info:eu-repo/semantics/altIdentifier/doi/10.1088/1742-6596/90/1/012044info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:22:31Zoai:sedici.unlp.edu.ar:10915/127391Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:22:31.654SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
title Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
spellingShingle Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
Reigosa, Miguel Antonio
Ciencias Exactas
Biología
cytotoxicity
cytocompatibility
genotoxicity
dental implants
osteoblasts
title_short Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
title_full Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
title_fullStr Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
title_full_unstemmed Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
title_sort Cytocompatibility, cytotoxicity and genotoxicity analysis of dental implants
dc.creator.none.fl_str_mv Reigosa, Miguel Antonio
Labarta, Verónica
Molinari, Gabriela
Bernales, D.
author Reigosa, Miguel Antonio
author_facet Reigosa, Miguel Antonio
Labarta, Verónica
Molinari, Gabriela
Bernales, D.
author_role author
author2 Labarta, Verónica
Molinari, Gabriela
Bernales, D.
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
cytotoxicity
cytocompatibility
genotoxicity
dental implants
osteoblasts
topic Ciencias Exactas
Biología
cytotoxicity
cytocompatibility
genotoxicity
dental implants
osteoblasts
dc.description.none.fl_txt_mv Several types of materials are frequently used for dental prostheses in dental medicine. Different treatments with titanium are the most used. The aim of the present study was to analyze by means of cytotoxicity and cytocompatibility techniques the capacity of dental implants to integrate to the bone tissue. Cultures of UMR 106 cell line derived from an osteosarcoma were used for bioassays mainly because they show many of the properties of osteoblasts. Dental implant samples provided by B&W company were compared with others of recognized trademarks. The first ones contain ASTM titanium (8348 GR2) with acid printing. Cytotoxicity was analyzed by means of lysosome activity, using the neutral red technique and alkaline phosphatase enzyme activity. Cell variability was determined by means of the acridine ethidium-orange bromide technique. One-way ANOVA and Bonferroni and Duncan post-ANOVA tests were used for the statistical analysis. The assays did not show significant differences among the dental implants analyzed. Our findings show that the dental prostheses studied present high biocompatibility, quantified by the bioassays performed. The techniques employed revealed that they can be a useful tool for the analysis of other materials for dental medicine use.
Instituto Multidisciplinario de Biología Celular
description Several types of materials are frequently used for dental prostheses in dental medicine. Different treatments with titanium are the most used. The aim of the present study was to analyze by means of cytotoxicity and cytocompatibility techniques the capacity of dental implants to integrate to the bone tissue. Cultures of UMR 106 cell line derived from an osteosarcoma were used for bioassays mainly because they show many of the properties of osteoblasts. Dental implant samples provided by B&W company were compared with others of recognized trademarks. The first ones contain ASTM titanium (8348 GR2) with acid printing. Cytotoxicity was analyzed by means of lysosome activity, using the neutral red technique and alkaline phosphatase enzyme activity. Cell variability was determined by means of the acridine ethidium-orange bromide technique. One-way ANOVA and Bonferroni and Duncan post-ANOVA tests were used for the statistical analysis. The assays did not show significant differences among the dental implants analyzed. Our findings show that the dental prostheses studied present high biocompatibility, quantified by the bioassays performed. The techniques employed revealed that they can be a useful tool for the analysis of other materials for dental medicine use.
publishDate 2007
dc.date.none.fl_str_mv 2007-11-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127391
url http://sedici.unlp.edu.ar/handle/10915/127391
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1742-6596
info:eu-repo/semantics/altIdentifier/doi/10.1088/1742-6596/90/1/012044
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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