A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba

Autores
González, N.V.; Nikoloff, Noelia; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this study was to further investigate the cytotoxic and genotoxic effects of dicamba and Banvel® employing the cytokinesis-block micronucleus cytome (CBMN-cyt) assay estimated by the analysis of the nuclear division index (NDI), the frequency of micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs). Besides, for mechanism of MN induction CREST anti-kinetochore antibody analysis was performed. The activities of both compounds were tested within the range of 50–500 μg/ml on Chinese hamster ovary (CHO-K1) cells. Overall, dicamba and Banvel® produced a NDI dose-dependent decrease but the response was statistically significant only in cultures treated with Banvel® at a 100–500 μg/ml concentration range. A dose-dependent induction of MN was observed after dicamba- and Banvel®-treatments within the 50–400 μg/ml and 50–500 μg/ml concentration-ranges, respectively. Induction of NPBs and NBUDs was significantly enhanced by both test compounds. The NPBs/MN ratio values found for dicamba and Banvel® were 0.04–0.11 and 0.05–0.18, respectively. Results clearly demonstrated that dicamba and Banvel® exerted both cyto- and genotoxic damage on CHO-K1 cells. Furthermore, the CBMN-cyt assay employed confirmed our previous investigations concerning the cellular and DNA damaging capabilities of dicamba and highlights that both clastogenic and aneugenic mechanisms are implicated in the MN induction.
Fil: González, N.V.. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
GENOTOXICITY
CYTOTOXICITY
DICAMBA
BANVEL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/95667
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/95667
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicambaGonzález, N.V.Nikoloff, NoeliaSoloneski, Sonia Maria ElsaLarramendy, Marcelo LuisGENOTOXICITYCYTOTOXICITYDICAMBABANVELhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The purpose of this study was to further investigate the cytotoxic and genotoxic effects of dicamba and Banvel® employing the cytokinesis-block micronucleus cytome (CBMN-cyt) assay estimated by the analysis of the nuclear division index (NDI), the frequency of micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs). Besides, for mechanism of MN induction CREST anti-kinetochore antibody analysis was performed. The activities of both compounds were tested within the range of 50–500 μg/ml on Chinese hamster ovary (CHO-K1) cells. Overall, dicamba and Banvel® produced a NDI dose-dependent decrease but the response was statistically significant only in cultures treated with Banvel® at a 100–500 μg/ml concentration range. A dose-dependent induction of MN was observed after dicamba- and Banvel®-treatments within the 50–400 μg/ml and 50–500 μg/ml concentration-ranges, respectively. Induction of NPBs and NBUDs was significantly enhanced by both test compounds. The NPBs/MN ratio values found for dicamba and Banvel® were 0.04–0.11 and 0.05–0.18, respectively. Results clearly demonstrated that dicamba and Banvel® exerted both cyto- and genotoxic damage on CHO-K1 cells. Furthermore, the CBMN-cyt assay employed confirmed our previous investigations concerning the cellular and DNA damaging capabilities of dicamba and highlights that both clastogenic and aneugenic mechanisms are implicated in the MN induction.Fil: González, N.V.. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; ArgentinaFil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Ireland2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/95667González, N.V.; Nikoloff, Noelia; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba; Elsevier Ireland; Toxicology Letters; 207; 3; 12-2011; 204-2120378-4274CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378427411015323info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxlet.2011.09.013info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:20Zoai:ri.conicet.gov.ar:11336/95667instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:20.247CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
title A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
spellingShingle A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
González, N.V.
GENOTOXICITY
CYTOTOXICITY
DICAMBA
BANVEL
title_short A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
title_full A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
title_fullStr A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
title_full_unstemmed A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
title_sort A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba
dc.creator.none.fl_str_mv González, N.V.
Nikoloff, Noelia
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author González, N.V.
author_facet González, N.V.
Nikoloff, Noelia
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author_role author
author2 Nikoloff, Noelia
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
author2_role author
author
author
dc.subject.none.fl_str_mv GENOTOXICITY
CYTOTOXICITY
DICAMBA
BANVEL
topic GENOTOXICITY
CYTOTOXICITY
DICAMBA
BANVEL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The purpose of this study was to further investigate the cytotoxic and genotoxic effects of dicamba and Banvel® employing the cytokinesis-block micronucleus cytome (CBMN-cyt) assay estimated by the analysis of the nuclear division index (NDI), the frequency of micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs). Besides, for mechanism of MN induction CREST anti-kinetochore antibody analysis was performed. The activities of both compounds were tested within the range of 50–500 μg/ml on Chinese hamster ovary (CHO-K1) cells. Overall, dicamba and Banvel® produced a NDI dose-dependent decrease but the response was statistically significant only in cultures treated with Banvel® at a 100–500 μg/ml concentration range. A dose-dependent induction of MN was observed after dicamba- and Banvel®-treatments within the 50–400 μg/ml and 50–500 μg/ml concentration-ranges, respectively. Induction of NPBs and NBUDs was significantly enhanced by both test compounds. The NPBs/MN ratio values found for dicamba and Banvel® were 0.04–0.11 and 0.05–0.18, respectively. Results clearly demonstrated that dicamba and Banvel® exerted both cyto- and genotoxic damage on CHO-K1 cells. Furthermore, the CBMN-cyt assay employed confirmed our previous investigations concerning the cellular and DNA damaging capabilities of dicamba and highlights that both clastogenic and aneugenic mechanisms are implicated in the MN induction.
Fil: González, N.V.. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Nikoloff, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Soloneski, Sonia Maria Elsa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina
Fil: Larramendy, Marcelo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Cátedra de Citología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The purpose of this study was to further investigate the cytotoxic and genotoxic effects of dicamba and Banvel® employing the cytokinesis-block micronucleus cytome (CBMN-cyt) assay estimated by the analysis of the nuclear division index (NDI), the frequency of micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs). Besides, for mechanism of MN induction CREST anti-kinetochore antibody analysis was performed. The activities of both compounds were tested within the range of 50–500 μg/ml on Chinese hamster ovary (CHO-K1) cells. Overall, dicamba and Banvel® produced a NDI dose-dependent decrease but the response was statistically significant only in cultures treated with Banvel® at a 100–500 μg/ml concentration range. A dose-dependent induction of MN was observed after dicamba- and Banvel®-treatments within the 50–400 μg/ml and 50–500 μg/ml concentration-ranges, respectively. Induction of NPBs and NBUDs was significantly enhanced by both test compounds. The NPBs/MN ratio values found for dicamba and Banvel® were 0.04–0.11 and 0.05–0.18, respectively. Results clearly demonstrated that dicamba and Banvel® exerted both cyto- and genotoxic damage on CHO-K1 cells. Furthermore, the CBMN-cyt assay employed confirmed our previous investigations concerning the cellular and DNA damaging capabilities of dicamba and highlights that both clastogenic and aneugenic mechanisms are implicated in the MN induction.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/95667
González, N.V.; Nikoloff, Noelia; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba; Elsevier Ireland; Toxicology Letters; 207; 3; 12-2011; 204-212
0378-4274
CONICET Digital
CONICET
url http://hdl.handle.net/11336/95667
identifier_str_mv González, N.V.; Nikoloff, Noelia; Soloneski, Sonia Maria Elsa; Larramendy, Marcelo Luis; A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba; Elsevier Ireland; Toxicology Letters; 207; 3; 12-2011; 204-212
0378-4274
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378427411015323
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxlet.2011.09.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269279449251840
score 13.13397

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