Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells

Autores
Riedel, Rodrigo Nicolas; Pérez Amodio, Soledad; Cabo Zabala, Laura; Velasco Ortega, Eugenio; Maymo, Julieta Lorena; Gil, Javier; Monsalve Guil, Loreto; Ortiz Garcia, Iván; Pérez Pérez, Antonio; Sánchez Margalet, Victor; Jiménez Guerra, Alvaro
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The dental implant surface plays a crucial role in osseointegration. The topography and physicochemical properties will affect the cellular functions. In this research, four distinct titanium surfaces have been studied: machined acting (MACH), acid etched (AE), grit blasting (GBLAST), and a combination of grit blasting and subsequent acid etching (GBLAST + AE). Human amniotic mesenchymal (hAMSCs) and epithelial stem cells (hAECs) isolated from the amniotic membrane have attractive stem-cell properties. They were cultured on titanium surfaces to analyze their impact on biological behavior. The surface roughness, microhardness, wettability, and surface energy were analyzed using interferometric microscopy, Vickers indentation, and drop-sessile techniques. The GBLAST and GBLAST + AE surfaces showed higher roughness, reduced hydrophilicity, and lower surface energy with significant differences. Increased microhardness values for GBLAST and GBLAST + AE implants were attributed to surface compression. Cell viability was higher for hAMSCs, particularly on GBLAST and GBLAST + AE surfaces. Alkaline phosphatase activity enhanced in hAMSCs cultured on GBLAST and GBLAST + AE surfaces, while hAECs showed no mineralization signals. Osteogenic gene expression was upregulated in hAMSCs on GBLAST surfaces. Moreover, α2 and β1 integrin expression enhanced in hAMSCs, suggesting a surface-integrin interaction. Consequently, hAMSCs would tend toward osteoblastic differentiation on grit-blasted surfaces conducive to osseointegration, a phenomenon not observed in hAECs.
Fil: Riedel, Rodrigo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pérez Amodio, Soledad. Universidad Internacional de Cataluña; España
Fil: Cabo Zabala, Laura. Instituto de Investigacion Biomédica de Malaga; España
Fil: Velasco Ortega, Eugenio. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gil, Javier. Universidad Internacional de Cataluña; España
Fil: Monsalve Guil, Loreto. Universidad de Sevilla; España
Fil: Ortiz Garcia, Iván. Universidad de Sevilla; España
Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
Fil: Sánchez Margalet, Victor. Universidad de Sevilla; España
Fil: Jiménez Guerra, Alvaro. Universidad de Sevilla; España
Materia
DENTAL IMPLANTS
AMNIOTIC MEMBRANE
OSTEOBLASTIC DIFFERENTIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266201
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/266201
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem CellsRiedel, Rodrigo NicolasPérez Amodio, SoledadCabo Zabala, LauraVelasco Ortega, EugenioMaymo, Julieta LorenaGil, JavierMonsalve Guil, LoretoOrtiz Garcia, IvánPérez Pérez, AntonioSánchez Margalet, VictorJiménez Guerra, AlvaroDENTAL IMPLANTSAMNIOTIC MEMBRANEOSTEOBLASTIC DIFFERENTIATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The dental implant surface plays a crucial role in osseointegration. The topography and physicochemical properties will affect the cellular functions. In this research, four distinct titanium surfaces have been studied: machined acting (MACH), acid etched (AE), grit blasting (GBLAST), and a combination of grit blasting and subsequent acid etching (GBLAST + AE). Human amniotic mesenchymal (hAMSCs) and epithelial stem cells (hAECs) isolated from the amniotic membrane have attractive stem-cell properties. They were cultured on titanium surfaces to analyze their impact on biological behavior. The surface roughness, microhardness, wettability, and surface energy were analyzed using interferometric microscopy, Vickers indentation, and drop-sessile techniques. The GBLAST and GBLAST + AE surfaces showed higher roughness, reduced hydrophilicity, and lower surface energy with significant differences. Increased microhardness values for GBLAST and GBLAST + AE implants were attributed to surface compression. Cell viability was higher for hAMSCs, particularly on GBLAST and GBLAST + AE surfaces. Alkaline phosphatase activity enhanced in hAMSCs cultured on GBLAST and GBLAST + AE surfaces, while hAECs showed no mineralization signals. Osteogenic gene expression was upregulated in hAMSCs on GBLAST surfaces. Moreover, α2 and β1 integrin expression enhanced in hAMSCs, suggesting a surface-integrin interaction. Consequently, hAMSCs would tend toward osteoblastic differentiation on grit-blasted surfaces conducive to osseointegration, a phenomenon not observed in hAECs.Fil: Riedel, Rodrigo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Pérez Amodio, Soledad. Universidad Internacional de Cataluña; EspañaFil: Cabo Zabala, Laura. Instituto de Investigacion Biomédica de Malaga; EspañaFil: Velasco Ortega, Eugenio. Universidad de Sevilla; EspañaFil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; ArgentinaFil: Gil, Javier. Universidad Internacional de Cataluña; EspañaFil: Monsalve Guil, Loreto. Universidad de Sevilla; EspañaFil: Ortiz Garcia, Iván. Universidad de Sevilla; EspañaFil: Pérez Pérez, Antonio. Universidad de Sevilla; EspañaFil: Sánchez Margalet, Victor. Universidad de Sevilla; EspañaFil: Jiménez Guerra, Alvaro. Universidad de Sevilla; EspañaMolecular Diversity Preservation International2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266201Riedel, Rodrigo Nicolas; Pérez Amodio, Soledad; Cabo Zabala, Laura; Velasco Ortega, Eugenio; Maymo, Julieta Lorena; et al.; Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 13; 7-2024; 1-171422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/13/7416info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25137416info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:27:10Zoai:ri.conicet.gov.ar:11336/266201instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:27:10.452CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
title Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
spellingShingle Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
Riedel, Rodrigo Nicolas
DENTAL IMPLANTS
AMNIOTIC MEMBRANE
OSTEOBLASTIC DIFFERENTIATION
title_short Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
title_full Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
title_fullStr Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
title_full_unstemmed Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
title_sort Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells
dc.creator.none.fl_str_mv Riedel, Rodrigo Nicolas
Pérez Amodio, Soledad
Cabo Zabala, Laura
Velasco Ortega, Eugenio
Maymo, Julieta Lorena
Gil, Javier
Monsalve Guil, Loreto
Ortiz Garcia, Iván
Pérez Pérez, Antonio
Sánchez Margalet, Victor
Jiménez Guerra, Alvaro
author Riedel, Rodrigo Nicolas
author_facet Riedel, Rodrigo Nicolas
Pérez Amodio, Soledad
Cabo Zabala, Laura
Velasco Ortega, Eugenio
Maymo, Julieta Lorena
Gil, Javier
Monsalve Guil, Loreto
Ortiz Garcia, Iván
Pérez Pérez, Antonio
Sánchez Margalet, Victor
Jiménez Guerra, Alvaro
author_role author
author2 Pérez Amodio, Soledad
Cabo Zabala, Laura
Velasco Ortega, Eugenio
Maymo, Julieta Lorena
Gil, Javier
Monsalve Guil, Loreto
Ortiz Garcia, Iván
Pérez Pérez, Antonio
Sánchez Margalet, Victor
Jiménez Guerra, Alvaro
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DENTAL IMPLANTS
AMNIOTIC MEMBRANE
OSTEOBLASTIC DIFFERENTIATION
topic DENTAL IMPLANTS
AMNIOTIC MEMBRANE
OSTEOBLASTIC DIFFERENTIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The dental implant surface plays a crucial role in osseointegration. The topography and physicochemical properties will affect the cellular functions. In this research, four distinct titanium surfaces have been studied: machined acting (MACH), acid etched (AE), grit blasting (GBLAST), and a combination of grit blasting and subsequent acid etching (GBLAST + AE). Human amniotic mesenchymal (hAMSCs) and epithelial stem cells (hAECs) isolated from the amniotic membrane have attractive stem-cell properties. They were cultured on titanium surfaces to analyze their impact on biological behavior. The surface roughness, microhardness, wettability, and surface energy were analyzed using interferometric microscopy, Vickers indentation, and drop-sessile techniques. The GBLAST and GBLAST + AE surfaces showed higher roughness, reduced hydrophilicity, and lower surface energy with significant differences. Increased microhardness values for GBLAST and GBLAST + AE implants were attributed to surface compression. Cell viability was higher for hAMSCs, particularly on GBLAST and GBLAST + AE surfaces. Alkaline phosphatase activity enhanced in hAMSCs cultured on GBLAST and GBLAST + AE surfaces, while hAECs showed no mineralization signals. Osteogenic gene expression was upregulated in hAMSCs on GBLAST surfaces. Moreover, α2 and β1 integrin expression enhanced in hAMSCs, suggesting a surface-integrin interaction. Consequently, hAMSCs would tend toward osteoblastic differentiation on grit-blasted surfaces conducive to osseointegration, a phenomenon not observed in hAECs.
Fil: Riedel, Rodrigo Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Pérez Amodio, Soledad. Universidad Internacional de Cataluña; España
Fil: Cabo Zabala, Laura. Instituto de Investigacion Biomédica de Malaga; España
Fil: Velasco Ortega, Eugenio. Universidad de Sevilla; España
Fil: Maymo, Julieta Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
Fil: Gil, Javier. Universidad Internacional de Cataluña; España
Fil: Monsalve Guil, Loreto. Universidad de Sevilla; España
Fil: Ortiz Garcia, Iván. Universidad de Sevilla; España
Fil: Pérez Pérez, Antonio. Universidad de Sevilla; España
Fil: Sánchez Margalet, Victor. Universidad de Sevilla; España
Fil: Jiménez Guerra, Alvaro. Universidad de Sevilla; España
description The dental implant surface plays a crucial role in osseointegration. The topography and physicochemical properties will affect the cellular functions. In this research, four distinct titanium surfaces have been studied: machined acting (MACH), acid etched (AE), grit blasting (GBLAST), and a combination of grit blasting and subsequent acid etching (GBLAST + AE). Human amniotic mesenchymal (hAMSCs) and epithelial stem cells (hAECs) isolated from the amniotic membrane have attractive stem-cell properties. They were cultured on titanium surfaces to analyze their impact on biological behavior. The surface roughness, microhardness, wettability, and surface energy were analyzed using interferometric microscopy, Vickers indentation, and drop-sessile techniques. The GBLAST and GBLAST + AE surfaces showed higher roughness, reduced hydrophilicity, and lower surface energy with significant differences. Increased microhardness values for GBLAST and GBLAST + AE implants were attributed to surface compression. Cell viability was higher for hAMSCs, particularly on GBLAST and GBLAST + AE surfaces. Alkaline phosphatase activity enhanced in hAMSCs cultured on GBLAST and GBLAST + AE surfaces, while hAECs showed no mineralization signals. Osteogenic gene expression was upregulated in hAMSCs on GBLAST surfaces. Moreover, α2 and β1 integrin expression enhanced in hAMSCs, suggesting a surface-integrin interaction. Consequently, hAMSCs would tend toward osteoblastic differentiation on grit-blasted surfaces conducive to osseointegration, a phenomenon not observed in hAECs.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266201
Riedel, Rodrigo Nicolas; Pérez Amodio, Soledad; Cabo Zabala, Laura; Velasco Ortega, Eugenio; Maymo, Julieta Lorena; et al.; Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 13; 7-2024; 1-17
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266201
identifier_str_mv Riedel, Rodrigo Nicolas; Pérez Amodio, Soledad; Cabo Zabala, Laura; Velasco Ortega, Eugenio; Maymo, Julieta Lorena; et al.; Influence of the Surface Topography of Titanium Dental Implants on the Behavior of Human Amniotic Stem Cells; Molecular Diversity Preservation International; International Journal of Molecular Sciences; 25; 13; 7-2024; 1-17
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/25/13/7416
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms25137416
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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