Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation

Autores
Gregorutti, Ricardo Walter; Grau, Jorge Enrique; Castrogiovanni, Daniel Cayetano; Parisi, Julieta Marcia; Reigosa, Miguel Antonio; Elsner, Cecilia Ines
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Body fluids are highly corrosive as they contain chlorides and hydroxides ions, as well as salts, bacteria, proteins and dissolved oxygen. The pH of the body is usually around 7.4, although this value can vary in a range of 4 to 9 after surgery or because of haematomas, inflammations and infections. ASTM F745 (type 316L) stainless steel has been used for load bearing partial and total joint replacements and post trauma reconstructive surgeries. However, long exposure to the aggressive effect of chloride ion present in the human body, may increase the susceptibility to suffer localized corrosion. Although UNS S32750 has greater corrosion resistance to chloride ion, its magnetic characteristics inhibit its use in implantable devices. Nevertheless, this stainless steel could be used in temporary implants and orthodontic appliances such as brackets, wire arches and bands, due to its high resistance to corrosion, the greater mechanical resistance and the high capacity of plastic forming. Objectives: The objective was to evaluate the susceptibility to localized corrosion in simulated body fluid, in the pH range of 4 to 9. Another objective was to evaluate the cytotoxicity of Cr and Ni present in the chemical composition of both stainless steels. Cytocompatibility was also analysed by seeding cells on the surfaces of both stainless steels. Methods: Cyclic polariation test was performed to evaluate the susceptibility to localized corrosion in 0.9 wt% NaCl aqueous solution, at pH between 4 and 9, maintained at 37°C. For cytotoxicity evaluation, neutral red, MTT and collagen assays were performed using UMR-106 cell line. Cytocompatibility was analysed by seeding UMR-106 cells on the surfaces of both stainless steels. Results: F745-SS was more susceptible to suffer localized corrosion than UNS S32750. Although it showed a tendency to develop transpassive reactions at low pH, galvanostatic tests did not reveal the onset of localized corrosion. The results from the cytotoxicity assays indicated that no adverse effects were observed. UMR-106 osteoblastic cells showed high viability, however, a slight reduction in the collagen production was observed. The cytocompatibility was also satisfactory, since the cells seeded on the surfaces had adequate proliferation. Conclusion: F745-SS is more susceptible to suffer localized corrosion than UNS S32750 in the pH range between 4 and 9. UNS S32750 showed an extensive passive region, however, transpassive reactions were observed at lower pH. On the other hand, no cytotoxic effects were promoted by both stainless steels, although a slight reduction in collagen production was observed. Cells seeded on F745-SS and UNS S32750 surfaces had an acceptable proliferation, without evidence of changes in their morphology.
Fil: Gregorutti, Ricardo Walter. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Laboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológica; Argentina
Fil: Grau, Jorge Enrique. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Laboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológica; Argentina
Fil: Castrogiovanni, Daniel Cayetano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Parisi, Julieta Marcia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Reigosa, Miguel Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Elsner, Cecilia Ines. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Materia
BIOMATERIALS
STAINLESS STEELS
LOCALIZED CORROSION
TRANSPASSIVE REACTIONS CYTOTOXICITY
CYTOCOMPATIBILITY
PROLIFERATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/146757

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oai_identifier_str oai:ri.conicet.gov.ar:11336/146757
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluationGregorutti, Ricardo WalterGrau, Jorge EnriqueCastrogiovanni, Daniel CayetanoParisi, Julieta MarciaReigosa, Miguel AntonioElsner, Cecilia InesBIOMATERIALSSTAINLESS STEELSLOCALIZED CORROSIONTRANSPASSIVE REACTIONS CYTOTOXICITYCYTOCOMPATIBILITYPROLIFERATIONhttps://purl.org/becyt/ford/2.5https://purl.org/becyt/ford/2Background: Body fluids are highly corrosive as they contain chlorides and hydroxides ions, as well as salts, bacteria, proteins and dissolved oxygen. The pH of the body is usually around 7.4, although this value can vary in a range of 4 to 9 after surgery or because of haematomas, inflammations and infections. ASTM F745 (type 316L) stainless steel has been used for load bearing partial and total joint replacements and post trauma reconstructive surgeries. However, long exposure to the aggressive effect of chloride ion present in the human body, may increase the susceptibility to suffer localized corrosion. Although UNS S32750 has greater corrosion resistance to chloride ion, its magnetic characteristics inhibit its use in implantable devices. Nevertheless, this stainless steel could be used in temporary implants and orthodontic appliances such as brackets, wire arches and bands, due to its high resistance to corrosion, the greater mechanical resistance and the high capacity of plastic forming. Objectives: The objective was to evaluate the susceptibility to localized corrosion in simulated body fluid, in the pH range of 4 to 9. Another objective was to evaluate the cytotoxicity of Cr and Ni present in the chemical composition of both stainless steels. Cytocompatibility was also analysed by seeding cells on the surfaces of both stainless steels. Methods: Cyclic polariation test was performed to evaluate the susceptibility to localized corrosion in 0.9 wt% NaCl aqueous solution, at pH between 4 and 9, maintained at 37°C. For cytotoxicity evaluation, neutral red, MTT and collagen assays were performed using UMR-106 cell line. Cytocompatibility was analysed by seeding UMR-106 cells on the surfaces of both stainless steels. Results: F745-SS was more susceptible to suffer localized corrosion than UNS S32750. Although it showed a tendency to develop transpassive reactions at low pH, galvanostatic tests did not reveal the onset of localized corrosion. The results from the cytotoxicity assays indicated that no adverse effects were observed. UMR-106 osteoblastic cells showed high viability, however, a slight reduction in the collagen production was observed. The cytocompatibility was also satisfactory, since the cells seeded on the surfaces had adequate proliferation. Conclusion: F745-SS is more susceptible to suffer localized corrosion than UNS S32750 in the pH range between 4 and 9. UNS S32750 showed an extensive passive region, however, transpassive reactions were observed at lower pH. On the other hand, no cytotoxic effects were promoted by both stainless steels, although a slight reduction in collagen production was observed. Cells seeded on F745-SS and UNS S32750 surfaces had an acceptable proliferation, without evidence of changes in their morphology.Fil: Gregorutti, Ricardo Walter. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Laboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológica; ArgentinaFil: Grau, Jorge Enrique. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Laboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológica; ArgentinaFil: Castrogiovanni, Daniel Cayetano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Parisi, Julieta Marcia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Reigosa, Miguel Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; ArgentinaFil: Elsner, Cecilia Ines. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaBenthan Science2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/146757Gregorutti, Ricardo Walter; Grau, Jorge Enrique; Castrogiovanni, Daniel Cayetano; Parisi, Julieta Marcia; Reigosa, Miguel Antonio; et al.; Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation; Benthan Science; Innovations in Corrosion and Materials Science; 10; 1; 5-2020; 47-572352-0949CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/182219/articleinfo:eu-repo/semantics/altIdentifier/doi/10.2174/2352094910999200523153527info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:57Zoai:ri.conicet.gov.ar:11336/146757instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:57.354CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
title Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
spellingShingle Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
Gregorutti, Ricardo Walter
BIOMATERIALS
STAINLESS STEELS
LOCALIZED CORROSION
TRANSPASSIVE REACTIONS CYTOTOXICITY
CYTOCOMPATIBILITY
PROLIFERATION
title_short Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
title_full Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
title_fullStr Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
title_full_unstemmed Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
title_sort Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation
dc.creator.none.fl_str_mv Gregorutti, Ricardo Walter
Grau, Jorge Enrique
Castrogiovanni, Daniel Cayetano
Parisi, Julieta Marcia
Reigosa, Miguel Antonio
Elsner, Cecilia Ines
author Gregorutti, Ricardo Walter
author_facet Gregorutti, Ricardo Walter
Grau, Jorge Enrique
Castrogiovanni, Daniel Cayetano
Parisi, Julieta Marcia
Reigosa, Miguel Antonio
Elsner, Cecilia Ines
author_role author
author2 Grau, Jorge Enrique
Castrogiovanni, Daniel Cayetano
Parisi, Julieta Marcia
Reigosa, Miguel Antonio
Elsner, Cecilia Ines
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOMATERIALS
STAINLESS STEELS
LOCALIZED CORROSION
TRANSPASSIVE REACTIONS CYTOTOXICITY
CYTOCOMPATIBILITY
PROLIFERATION
topic BIOMATERIALS
STAINLESS STEELS
LOCALIZED CORROSION
TRANSPASSIVE REACTIONS CYTOTOXICITY
CYTOCOMPATIBILITY
PROLIFERATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.5
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Body fluids are highly corrosive as they contain chlorides and hydroxides ions, as well as salts, bacteria, proteins and dissolved oxygen. The pH of the body is usually around 7.4, although this value can vary in a range of 4 to 9 after surgery or because of haematomas, inflammations and infections. ASTM F745 (type 316L) stainless steel has been used for load bearing partial and total joint replacements and post trauma reconstructive surgeries. However, long exposure to the aggressive effect of chloride ion present in the human body, may increase the susceptibility to suffer localized corrosion. Although UNS S32750 has greater corrosion resistance to chloride ion, its magnetic characteristics inhibit its use in implantable devices. Nevertheless, this stainless steel could be used in temporary implants and orthodontic appliances such as brackets, wire arches and bands, due to its high resistance to corrosion, the greater mechanical resistance and the high capacity of plastic forming. Objectives: The objective was to evaluate the susceptibility to localized corrosion in simulated body fluid, in the pH range of 4 to 9. Another objective was to evaluate the cytotoxicity of Cr and Ni present in the chemical composition of both stainless steels. Cytocompatibility was also analysed by seeding cells on the surfaces of both stainless steels. Methods: Cyclic polariation test was performed to evaluate the susceptibility to localized corrosion in 0.9 wt% NaCl aqueous solution, at pH between 4 and 9, maintained at 37°C. For cytotoxicity evaluation, neutral red, MTT and collagen assays were performed using UMR-106 cell line. Cytocompatibility was analysed by seeding UMR-106 cells on the surfaces of both stainless steels. Results: F745-SS was more susceptible to suffer localized corrosion than UNS S32750. Although it showed a tendency to develop transpassive reactions at low pH, galvanostatic tests did not reveal the onset of localized corrosion. The results from the cytotoxicity assays indicated that no adverse effects were observed. UMR-106 osteoblastic cells showed high viability, however, a slight reduction in the collagen production was observed. The cytocompatibility was also satisfactory, since the cells seeded on the surfaces had adequate proliferation. Conclusion: F745-SS is more susceptible to suffer localized corrosion than UNS S32750 in the pH range between 4 and 9. UNS S32750 showed an extensive passive region, however, transpassive reactions were observed at lower pH. On the other hand, no cytotoxic effects were promoted by both stainless steels, although a slight reduction in collagen production was observed. Cells seeded on F745-SS and UNS S32750 surfaces had an acceptable proliferation, without evidence of changes in their morphology.
Fil: Gregorutti, Ricardo Walter. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Laboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológica; Argentina
Fil: Grau, Jorge Enrique. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Laboratorio de Entrenamiento Multidisciplinario para la Investigación Tecnológica; Argentina
Fil: Castrogiovanni, Daniel Cayetano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Parisi, Julieta Marcia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Reigosa, Miguel Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina
Fil: Elsner, Cecilia Ines. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
description Background: Body fluids are highly corrosive as they contain chlorides and hydroxides ions, as well as salts, bacteria, proteins and dissolved oxygen. The pH of the body is usually around 7.4, although this value can vary in a range of 4 to 9 after surgery or because of haematomas, inflammations and infections. ASTM F745 (type 316L) stainless steel has been used for load bearing partial and total joint replacements and post trauma reconstructive surgeries. However, long exposure to the aggressive effect of chloride ion present in the human body, may increase the susceptibility to suffer localized corrosion. Although UNS S32750 has greater corrosion resistance to chloride ion, its magnetic characteristics inhibit its use in implantable devices. Nevertheless, this stainless steel could be used in temporary implants and orthodontic appliances such as brackets, wire arches and bands, due to its high resistance to corrosion, the greater mechanical resistance and the high capacity of plastic forming. Objectives: The objective was to evaluate the susceptibility to localized corrosion in simulated body fluid, in the pH range of 4 to 9. Another objective was to evaluate the cytotoxicity of Cr and Ni present in the chemical composition of both stainless steels. Cytocompatibility was also analysed by seeding cells on the surfaces of both stainless steels. Methods: Cyclic polariation test was performed to evaluate the susceptibility to localized corrosion in 0.9 wt% NaCl aqueous solution, at pH between 4 and 9, maintained at 37°C. For cytotoxicity evaluation, neutral red, MTT and collagen assays were performed using UMR-106 cell line. Cytocompatibility was analysed by seeding UMR-106 cells on the surfaces of both stainless steels. Results: F745-SS was more susceptible to suffer localized corrosion than UNS S32750. Although it showed a tendency to develop transpassive reactions at low pH, galvanostatic tests did not reveal the onset of localized corrosion. The results from the cytotoxicity assays indicated that no adverse effects were observed. UMR-106 osteoblastic cells showed high viability, however, a slight reduction in the collagen production was observed. The cytocompatibility was also satisfactory, since the cells seeded on the surfaces had adequate proliferation. Conclusion: F745-SS is more susceptible to suffer localized corrosion than UNS S32750 in the pH range between 4 and 9. UNS S32750 showed an extensive passive region, however, transpassive reactions were observed at lower pH. On the other hand, no cytotoxic effects were promoted by both stainless steels, although a slight reduction in collagen production was observed. Cells seeded on F745-SS and UNS S32750 surfaces had an acceptable proliferation, without evidence of changes in their morphology.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/146757
Gregorutti, Ricardo Walter; Grau, Jorge Enrique; Castrogiovanni, Daniel Cayetano; Parisi, Julieta Marcia; Reigosa, Miguel Antonio; et al.; Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation; Benthan Science; Innovations in Corrosion and Materials Science; 10; 1; 5-2020; 47-57
2352-0949
CONICET Digital
CONICET
url http://hdl.handle.net/11336/146757
identifier_str_mv Gregorutti, Ricardo Walter; Grau, Jorge Enrique; Castrogiovanni, Daniel Cayetano; Parisi, Julieta Marcia; Reigosa, Miguel Antonio; et al.; Susceptibility to localized corrosion of ASTM F745 and UNS S32750 stainless steels. influence of pH and cytotoxicity evaluation; Benthan Science; Innovations in Corrosion and Materials Science; 10; 1; 5-2020; 47-57
2352-0949
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.eurekaselect.com/182219/article
info:eu-repo/semantics/altIdentifier/doi/10.2174/2352094910999200523153527
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Benthan Science
publisher.none.fl_str_mv Benthan Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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