Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells

Autores
Castro, Marisa Silvia; Molina, Matías Alejandro; Azpiroz, María Belén; Díaz, Ailén Magalí; Ponzio, R.; Sparo, Mónica Delfina; Manghi, Marcela Alejandra; Canellada, Andrea Mercedes
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To analyse the effect of Enterococcus faecalis CECT7121 on intestinalepithelial cells (IECs) and its effects on the mucosal immune response. Methods and Results: Enterococcus faecalis CECT7121 showed a high adhesioncapacity to completely and heterogeneously differentiated human intestinalepithelial cell line (Caco-2 cells). In addition, the contact of this bacteriumwith Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA⁺ and IL-6⁺ cells in the small intestine, as well as theproduction of inflammatory cytokines (TNFa, IL-6 and IL-12) in the gut, wasdetermined after intragastric inoculation of Ent. faecalis CECT7121 in BALB/cmice. The administration of Ent. faecalis CECT7121 increased the number ofIgA⁺ cells in the intestinal lamina propria without modifying the percentage ofIL-6⁺ cells. No differences were observed in the cytokines measured in theintestinal extracts between probiotic-treated and control mice. Conclusions: Enterococcus faecalis CECT7121 stimulates local mucosalimmunity and adheres to IECs without inducing inflammatory signals. Significance and Impact of the Study: Our results indicate that, apart from itsalready reported systemic immune activity, Ent. faecalis CECT7121 has amodulatory effect at a local level.
Centro Universitario de Estudios Microbiológicos y Parasitológicos
Materia
Medicina
Ciencias Exactas
adhesion to intestinal epithelial cells
Enterococcus faecalis CECT7121
immunomodulation
mucosal immune response
probiotic activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/101711
Acceder
id SEDICI_e50be3620101674cb50fc4cecfd1848c
oai_identifier_str oai:sedici.unlp.edu.ar:10915/101711
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cellsCastro, Marisa SilviaMolina, Matías AlejandroAzpiroz, María BelénDíaz, Ailén MagalíPonzio, R.Sparo, Mónica DelfinaManghi, Marcela AlejandraCanellada, Andrea MercedesMedicinaCiencias Exactasadhesion to intestinal epithelial cellsEnterococcus faecalis CECT7121immunomodulationmucosal immune responseprobiotic activityAims: To analyse the effect of <i>Enterococcus faecalis</i> CECT7121 on intestinalepithelial cells (IECs) and its effects on the mucosal immune response. Methods and Results: <i>Enterococcus faecalis</i> CECT7121 showed a high adhesioncapacity to completely and heterogeneously differentiated human intestinalepithelial cell line (Caco-2 cells). In addition, the contact of this bacteriumwith Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA⁺ and IL-6⁺ cells in the small intestine, as well as theproduction of inflammatory cytokines (TNFa, IL-6 and IL-12) in the gut, wasdetermined after intragastric inoculation of <i>Ent. faecalis</i> CECT7121 in BALB/cmice. The administration of <i>Ent. faecalis</i> CECT7121 increased the number ofIgA⁺ cells in the intestinal lamina propria without modifying the percentage ofIL-6⁺ cells. No differences were observed in the cytokines measured in theintestinal extracts between probiotic-treated and control mice. Conclusions: <i>Enterococcus faecalis</i> CECT7121 stimulates local mucosalimmunity and adheres to IECs without inducing inflammatory signals. Significance and Impact of the Study: Our results indicate that, apart from itsalready reported systemic immune activity, <i>Ent. faecalis</i> CECT7121 has amodulatory effect at a local level.Centro Universitario de Estudios Microbiológicos y Parasitológicos2016-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1117-1129http://sedici.unlp.edu.ar/handle/10915/101711enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/47117info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13226info:eu-repo/semantics/altIdentifier/issn/1364-5072info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.13226info:eu-repo/semantics/altIdentifier/hdl/11336/47117info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:21:00Zoai:sedici.unlp.edu.ar:10915/101711Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:21:00.692SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
title Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
spellingShingle Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
Castro, Marisa Silvia
Medicina
Ciencias Exactas
adhesion to intestinal epithelial cells
Enterococcus faecalis CECT7121
immunomodulation
mucosal immune response
probiotic activity
title_short Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
title_full Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
title_fullStr Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
title_full_unstemmed Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
title_sort Probiotic activity of <i>Enterococcus faecalis</i> CECT7121: effects on mucosal immunity and intestinal epithelial cells
dc.creator.none.fl_str_mv Castro, Marisa Silvia
Molina, Matías Alejandro
Azpiroz, María Belén
Díaz, Ailén Magalí
Ponzio, R.
Sparo, Mónica Delfina
Manghi, Marcela Alejandra
Canellada, Andrea Mercedes
author Castro, Marisa Silvia
author_facet Castro, Marisa Silvia
Molina, Matías Alejandro
Azpiroz, María Belén
Díaz, Ailén Magalí
Ponzio, R.
Sparo, Mónica Delfina
Manghi, Marcela Alejandra
Canellada, Andrea Mercedes
author_role author
author2 Molina, Matías Alejandro
Azpiroz, María Belén
Díaz, Ailén Magalí
Ponzio, R.
Sparo, Mónica Delfina
Manghi, Marcela Alejandra
Canellada, Andrea Mercedes
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Ciencias Exactas
adhesion to intestinal epithelial cells
Enterococcus faecalis CECT7121
immunomodulation
mucosal immune response
probiotic activity
topic Medicina
Ciencias Exactas
adhesion to intestinal epithelial cells
Enterococcus faecalis CECT7121
immunomodulation
mucosal immune response
probiotic activity
dc.description.none.fl_txt_mv Aims: To analyse the effect of <i>Enterococcus faecalis</i> CECT7121 on intestinalepithelial cells (IECs) and its effects on the mucosal immune response. Methods and Results: <i>Enterococcus faecalis</i> CECT7121 showed a high adhesioncapacity to completely and heterogeneously differentiated human intestinalepithelial cell line (Caco-2 cells). In addition, the contact of this bacteriumwith Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA⁺ and IL-6⁺ cells in the small intestine, as well as theproduction of inflammatory cytokines (TNFa, IL-6 and IL-12) in the gut, wasdetermined after intragastric inoculation of <i>Ent. faecalis</i> CECT7121 in BALB/cmice. The administration of <i>Ent. faecalis</i> CECT7121 increased the number ofIgA⁺ cells in the intestinal lamina propria without modifying the percentage ofIL-6⁺ cells. No differences were observed in the cytokines measured in theintestinal extracts between probiotic-treated and control mice. Conclusions: <i>Enterococcus faecalis</i> CECT7121 stimulates local mucosalimmunity and adheres to IECs without inducing inflammatory signals. Significance and Impact of the Study: Our results indicate that, apart from itsalready reported systemic immune activity, <i>Ent. faecalis</i> CECT7121 has amodulatory effect at a local level.
Centro Universitario de Estudios Microbiológicos y Parasitológicos
description Aims: To analyse the effect of <i>Enterococcus faecalis</i> CECT7121 on intestinalepithelial cells (IECs) and its effects on the mucosal immune response. Methods and Results: <i>Enterococcus faecalis</i> CECT7121 showed a high adhesioncapacity to completely and heterogeneously differentiated human intestinalepithelial cell line (Caco-2 cells). In addition, the contact of this bacteriumwith Caco-2 cells did not induce inflammatory chemokines (IL-8 and CCL-20). The presence of IgA⁺ and IL-6⁺ cells in the small intestine, as well as theproduction of inflammatory cytokines (TNFa, IL-6 and IL-12) in the gut, wasdetermined after intragastric inoculation of <i>Ent. faecalis</i> CECT7121 in BALB/cmice. The administration of <i>Ent. faecalis</i> CECT7121 increased the number ofIgA⁺ cells in the intestinal lamina propria without modifying the percentage ofIL-6⁺ cells. No differences were observed in the cytokines measured in theintestinal extracts between probiotic-treated and control mice. Conclusions: <i>Enterococcus faecalis</i> CECT7121 stimulates local mucosalimmunity and adheres to IECs without inducing inflammatory signals. Significance and Impact of the Study: Our results indicate that, apart from itsalready reported systemic immune activity, <i>Ent. faecalis</i> CECT7121 has amodulatory effect at a local level.
publishDate 2016
dc.date.none.fl_str_mv 2016-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/101711
url http://sedici.unlp.edu.ar/handle/10915/101711
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/47117
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/jam.13226
info:eu-repo/semantics/altIdentifier/issn/1364-5072
info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.13226
info:eu-repo/semantics/altIdentifier/hdl/11336/47117
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1117-1129
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1844616084978663424
score 13.069144

Publicaciones similares