Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall

Autores
Confalonieri, Alejandra; Sparo, Mónica Delfina; Urbizu, Lucia Paola; Rivulgo, Virginia Margarita; Sanchez Bruni, Sergio Fabian
Año de publicación
2009
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se.
Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Rivulgo, Virginia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental
Materia
ENTEROCOCCUS FAECALIS CECT7121
CELL WALL EXTRACT
IMMUNOMODULATION
SALMONELLA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/243093
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell WallConfalonieri, AlejandraSparo, Mónica DelfinaUrbizu, Lucia PaolaRivulgo, Virginia MargaritaSanchez Bruni, Sergio FabianENTEROCOCCUS FAECALIS CECT7121CELL WALL EXTRACTIMMUNOMODULATIONSALMONELLAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se.Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Rivulgo, Virginia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaLXI Reunión Anual de Sociedad Argentina de Farmacología ExperimentalRosarioArgentinaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de Farmacología Experimental2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/243093Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall; LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:43Zoai:ri.conicet.gov.ar:11336/243093instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:43.286CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
title Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
spellingShingle Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
Confalonieri, Alejandra
ENTEROCOCCUS FAECALIS CECT7121
CELL WALL EXTRACT
IMMUNOMODULATION
SALMONELLA
title_short Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
title_full Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
title_fullStr Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
title_full_unstemmed Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
title_sort Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
dc.creator.none.fl_str_mv Confalonieri, Alejandra
Sparo, Mónica Delfina
Urbizu, Lucia Paola
Rivulgo, Virginia Margarita
Sanchez Bruni, Sergio Fabian
author Confalonieri, Alejandra
author_facet Confalonieri, Alejandra
Sparo, Mónica Delfina
Urbizu, Lucia Paola
Rivulgo, Virginia Margarita
Sanchez Bruni, Sergio Fabian
author_role author
author2 Sparo, Mónica Delfina
Urbizu, Lucia Paola
Rivulgo, Virginia Margarita
Sanchez Bruni, Sergio Fabian
author2_role author
author
author
author
dc.subject.none.fl_str_mv ENTEROCOCCUS FAECALIS CECT7121
CELL WALL EXTRACT
IMMUNOMODULATION
SALMONELLA
topic ENTEROCOCCUS FAECALIS CECT7121
CELL WALL EXTRACT
IMMUNOMODULATION
SALMONELLA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se.
Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Rivulgo, Virginia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental
description Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se.
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/243093
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall; LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32
CONICET Digital
CONICET
url http://hdl.handle.net/11336/243093
identifier_str_mv Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall; LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
publisher.none.fl_str_mv Sociedad Argentina de Farmacología Experimental
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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