Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall
- Autores
- Confalonieri, Alejandra; Sparo, Mónica Delfina; Urbizu, Lucia Paola; Rivulgo, Virginia Margarita; Sanchez Bruni, Sergio Fabian
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se.
Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Rivulgo, Virginia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental
Rosario
Argentina
Sociedad Argentina de Farmacología Experimental - Materia
-
ENTEROCOCCUS FAECALIS CECT7121
CELL WALL EXTRACT
IMMUNOMODULATION
SALMONELLA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/243093
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Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell WallConfalonieri, AlejandraSparo, Mónica DelfinaUrbizu, Lucia PaolaRivulgo, Virginia MargaritaSanchez Bruni, Sergio FabianENTEROCOCCUS FAECALIS CECT7121CELL WALL EXTRACTIMMUNOMODULATIONSALMONELLAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se.Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Rivulgo, Virginia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaFil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; ArgentinaLXI Reunión Anual de Sociedad Argentina de Farmacología ExperimentalRosarioArgentinaSociedad Argentina de Farmacología ExperimentalSociedad Argentina de Farmacología Experimental2009info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/243093Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall; LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:50Zoai:ri.conicet.gov.ar:11336/243093instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:50.767CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
title |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
spellingShingle |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall Confalonieri, Alejandra ENTEROCOCCUS FAECALIS CECT7121 CELL WALL EXTRACT IMMUNOMODULATION SALMONELLA |
title_short |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
title_full |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
title_fullStr |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
title_full_unstemmed |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
title_sort |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall |
dc.creator.none.fl_str_mv |
Confalonieri, Alejandra Sparo, Mónica Delfina Urbizu, Lucia Paola Rivulgo, Virginia Margarita Sanchez Bruni, Sergio Fabian |
author |
Confalonieri, Alejandra |
author_facet |
Confalonieri, Alejandra Sparo, Mónica Delfina Urbizu, Lucia Paola Rivulgo, Virginia Margarita Sanchez Bruni, Sergio Fabian |
author_role |
author |
author2 |
Sparo, Mónica Delfina Urbizu, Lucia Paola Rivulgo, Virginia Margarita Sanchez Bruni, Sergio Fabian |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
ENTEROCOCCUS FAECALIS CECT7121 CELL WALL EXTRACT IMMUNOMODULATION SALMONELLA |
topic |
ENTEROCOCCUS FAECALIS CECT7121 CELL WALL EXTRACT IMMUNOMODULATION SALMONELLA |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.3 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se. Fil: Confalonieri, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina Fil: Sparo, Mónica Delfina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina Fil: Urbizu, Lucia Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina Fil: Rivulgo, Virginia Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina Fil: Sanchez Bruni, Sergio Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental Rosario Argentina Sociedad Argentina de Farmacología Experimental |
description |
Several studies have demonstrated the utility of the bacterial cell wall extracts (CWE) as immunomodulators on the treatment of some infectious diseases. Enterococcus faecalis (Ef) CECT7121, a probiotic strain, also demonstrated immunomodulation in some animal models. The goal of this work was to obtain an extract of CWE of E. faecalis CECT7121 and to evaluate its efficacy on Salmonella enteritidis (Se) in mice model. Culture died by heat Ef CECT7121 was sonicated during 30 minutes and was put under three successive centrifugations to 27,000g during 1 h. The obtained CWE was freeze-dried. For the efficacy study, thirty Balb-c mice were divided in 3 groups (n=10) and treated as follows: Group I Control: challenged with 2 doses of Se 5x107 CFU/ml (LD99) q12 h and 5 doses of physiological solution q 24h. Group II: received only 5 doses of 1000 μg of CWE q 24h. Experimental Group III: was challenged as Group II and treated with 1000 μg of CWE q 24h. The survival rate was observed during 15 days post- challenge. Thirty percent of the animals of the Group III survived, compared with those of the Group I Control (100% of death). There was not observed changes on health of animals of the Group II, by which demonstrate the safety of the CWE. We conclude that CWE compound would display some immunomodulator properties responsible of the prolongation of the animal survival after challenging with a DL99 of Se. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Book http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/243093 Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall; LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/243093 |
identifier_str_mv |
Efficacy of an immunomodulator compound obtained from Enterococcus faecalis CECT7121 Cell Wall; LXI Reunión Anual de Sociedad Argentina de Farmacología Experimental; Rosario; Argentina; 2009; 32-32 CONICET Digital CONICET |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/url/https://aafeargentina.org/congresos-aafe/ |
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info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
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application/pdf application/msword application/pdf |
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Sociedad Argentina de Farmacología Experimental |
publisher.none.fl_str_mv |
Sociedad Argentina de Farmacología Experimental |
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reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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