Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord

Autores
Nishida, Fabián; Zanuzzi, Carolina Natalia; Martínez, Agustín; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25. mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KA-treated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1. mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.
Facultad de Ciencias Veterinarias
Laboratorio de Análisis de Imágenes
Materia
Veterinaria
Cervical segments
Kainic acid
Morphometric analysis
Rat
Spinal cord
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/99799
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/99799
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cordNishida, FabiánZanuzzi, Carolina NataliaMartínez, AgustínBarbeito, Claudio GustavoPortiansky, Enrique LeoVeterinariaCervical segmentsKainic acidMorphometric analysisRatSpinal cordKainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25. mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KA-treated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1. mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.Facultad de Ciencias VeterinariasLaboratorio de Análisis de Imágenes2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf68-78http://sedici.unlp.edu.ar/handle/10915/99799enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/53401info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0161813X15000820info:eu-repo/semantics/altIdentifier/issn/0161-813Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuro.2015.05.006info:eu-repo/semantics/altIdentifier/hdl/11336/53401info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:20:06Zoai:sedici.unlp.edu.ar:10915/99799Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:20:07.111SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
spellingShingle Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
Nishida, Fabián
Veterinaria
Cervical segments
Kainic acid
Morphometric analysis
Rat
Spinal cord
title_short Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_full Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_fullStr Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_full_unstemmed Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_sort Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
dc.creator.none.fl_str_mv Nishida, Fabián
Zanuzzi, Carolina Natalia
Martínez, Agustín
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author Nishida, Fabián
author_facet Nishida, Fabián
Zanuzzi, Carolina Natalia
Martínez, Agustín
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author_role author
author2 Zanuzzi, Carolina Natalia
Martínez, Agustín
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Veterinaria
Cervical segments
Kainic acid
Morphometric analysis
Rat
Spinal cord
topic Veterinaria
Cervical segments
Kainic acid
Morphometric analysis
Rat
Spinal cord
dc.description.none.fl_txt_mv Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25. mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KA-treated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1. mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.
Facultad de Ciencias Veterinarias
Laboratorio de Análisis de Imágenes
description Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25. mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KA-treated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1. mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/99799
url http://sedici.unlp.edu.ar/handle/10915/99799
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/53401
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0161813X15000820
info:eu-repo/semantics/altIdentifier/issn/0161-813X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neuro.2015.05.006
info:eu-repo/semantics/altIdentifier/hdl/11336/53401
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
68-78
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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