Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord

Autores
Nishida, Fabián; Zanuzzi, Carolina Natalia; Martinez, Agustin; Barbeito, Claudio Gustavo; Portiansky, Enrique Leo
Año de publicación
2015
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25 mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KAtreated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1 mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.
Estación Experimental Agropecuaria Bariloche
Fil: Nishida, Fabián. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Aropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
NeuroToxicology 49 : 68–78 (2015)
Materia
Sanidad Animal
Rata
Animal Health
Rats
Ácido Kaínico (KA)
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6708
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6708
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cordNishida, FabiánZanuzzi, Carolina NataliaMartinez, AgustinBarbeito, Claudio GustavoPortiansky, Enrique LeoSanidad AnimalRataAnimal HealthRatsÁcido Kaínico (KA)Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25 mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KAtreated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1 mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.Estación Experimental Agropecuaria BarilocheFil: Nishida, Fabián. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Agustin. Instituto Nacional de Tecnología Aropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; ArgentinaFil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2020-02-10T14:19:42Z2020-02-10T14:19:42Z2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6708https://www.sciencedirect.com/science/article/pii/S0161813X150008200161-813Xhttps://doi.org/10.1016/j.neuro.2015.05.006NeuroToxicology 49 : 68–78 (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:52Zoai:localhost:20.500.12123/6708instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:52.979INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
spellingShingle Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
Nishida, Fabián
Sanidad Animal
Rata
Animal Health
Rats
Ácido Kaínico (KA)
title_short Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_full Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_fullStr Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_full_unstemmed Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
title_sort Functional and histopathological changes induced by intraparenchymal injection of kainic acid in the rat cervical spinal cord
dc.creator.none.fl_str_mv Nishida, Fabián
Zanuzzi, Carolina Natalia
Martinez, Agustin
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author Nishida, Fabián
author_facet Nishida, Fabián
Zanuzzi, Carolina Natalia
Martinez, Agustin
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author_role author
author2 Zanuzzi, Carolina Natalia
Martinez, Agustin
Barbeito, Claudio Gustavo
Portiansky, Enrique Leo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sanidad Animal
Rata
Animal Health
Rats
Ácido Kaínico (KA)
topic Sanidad Animal
Rata
Animal Health
Rats
Ácido Kaínico (KA)
dc.description.none.fl_txt_mv Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25 mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KAtreated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1 mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.
Estación Experimental Agropecuaria Bariloche
Fil: Nishida, Fabián. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zanuzzi, Carolina Natalia. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Agustin. Instituto Nacional de Tecnología Aropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área Producción Animal. Grupo Sanidad Animal; Argentina
Fil: Barbeito, Claudio Gustavo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias; Argentina. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Departamento de Histología y Embriología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Portiansky, Enrique Leo. Universidad Nacional de La Plata. Escuela de Ciencias Veterinarias. Laboratorio de Análisis de Imágenes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Kainic acid (KA) is an analog of the neurotransmitter glutamate and is widely used as an excitotoxic agent to lesion spinal cord networks, thus, providing an interesting model to learn basic mechanisms of spinal cord injury. The present work was aimed to evaluate motor and sensory performance of rats and analyze morphometric parameters of spinal cord neurons after KA injection. Animals were injected either with 0.75, 1 or 1.25 mM of KA at the C5 segment of the cervical spinal cord. Motor and sensory performance of the rats were evaluate at day 0 (before injection) and at days 1, 2, 3 and 7 post-injection (pi) and compared with those of saline-treated and non-operated animals. Animals were sacrificed at each time point for morphometric and histopathological analysis and compared among groups. All KAtreated animals showed a significant impairment at the motor and sensory tests for the ipsilateral forelimb in a concentration-dependent manner in comparison to saline-treated and non-operated animals. Neuronal cell count showed a significant loss of neurons at C4, C5 and C6 cervical segments when compared with those of saline-treated and non-operated animals. The contralateral side of the cervical segments in KA-treated rats remained unchanged. Some improvement at the motor and sensory tests was observed in animals injected with 0.75 and 1 mM KA. Moreover, a mild increase in the neuronal count of the damaged segments was also recorded. The improvement recorded in the motor and sensory tests by day 7 pi may be a consequence of a neuron repairing mechanism triggered soon after the KA excitotoxic effect.
publishDate 2015
dc.date.none.fl_str_mv 2015-05
2020-02-10T14:19:42Z
2020-02-10T14:19:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6708
https://www.sciencedirect.com/science/article/pii/S0161813X15000820
0161-813X
https://doi.org/10.1016/j.neuro.2015.05.006
url http://hdl.handle.net/20.500.12123/6708
https://www.sciencedirect.com/science/article/pii/S0161813X15000820
https://doi.org/10.1016/j.neuro.2015.05.006
identifier_str_mv 0161-813X
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv NeuroToxicology 49 : 68–78 (2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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