Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord

Autores
Sisti, María Susana; Zanuzzi, Carolina Natalia; Nishida, Fabián; Cantet, Rodolfo Juan Carlos; Portiansky, Enrique Leo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lidocaine effects in the spinal cord have been extensively investigated over the years. Although the intrathecal route is usually used to treat insults occurring in the spinal cord, the local delivery drug via intraparenchymal infusions has gained increasing favor for the treatment of some neurodegenerative disorders. The aim of the present study was to evaluate the behavioral and tissue effects of the intraparenchymal injection of different concentrations of lidocaine into the rat cervical spinal cord. Young male Sprague-Dawley rats were intraparenchymally injected with 0.5%, 1% or 2% lidocaine at the C5 segment of the spinal cord. Other rats were injected with saline solution (sham group). Hot plate test was determined at 0, 1, 2, 3, 7 and 14 post-injection (pi) days. Rats of each experimental group were euthanized either at 1, 2, 3, 7 or 14 pi days. Intact animals were used as controls. Sections of the C5 segment were used for histological, immunohistochemical or immunofluorescence analysis. Injection of 0.5% lidocaine did not affect neuronal counting, did not evoke an inflammatory reaction, nor induce astrocyte activation. Therefore, a concentration of 0.5% lidocaine is suggested to promote anti-inflammatory effects after injury.
Laboratorio de Análisis de Imágenes
Materia
Ciencias Veterinarias
Spinal cord injury
Local anesthetic
Drug delivery
Toxic effect
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/131399
Acceder
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network_name_str SEDICI (UNLP)
spelling Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cordSisti, María SusanaZanuzzi, Carolina NataliaNishida, FabiánCantet, Rodolfo Juan CarlosPortiansky, Enrique LeoCiencias VeterinariasSpinal cord injuryLocal anestheticDrug deliveryToxic effectLidocaine effects in the spinal cord have been extensively investigated over the years. Although the intrathecal route is usually used to treat insults occurring in the spinal cord, the local delivery drug via intraparenchymal infusions has gained increasing favor for the treatment of some neurodegenerative disorders. The aim of the present study was to evaluate the behavioral and tissue effects of the intraparenchymal injection of different concentrations of lidocaine into the rat cervical spinal cord. Young male Sprague-Dawley rats were intraparenchymally injected with 0.5%, 1% or 2% lidocaine at the C5 segment of the spinal cord. Other rats were injected with saline solution (sham group). Hot plate test was determined at 0, 1, 2, 3, 7 and 14 post-injection (pi) days. Rats of each experimental group were euthanized either at 1, 2, 3, 7 or 14 pi days. Intact animals were used as controls. Sections of the C5 segment were used for histological, immunohistochemical or immunofluorescence analysis. Injection of 0.5% lidocaine did not affect neuronal counting, did not evoke an inflammatory reaction, nor induce astrocyte activation. Therefore, a concentration of 0.5% lidocaine is suggested to promote anti-inflammatory effects after injury.Laboratorio de Análisis de Imágenes2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2072-2080http://sedici.unlp.edu.ar/handle/10915/131399enginfo:eu-repo/semantics/altIdentifier/issn/1573-6903info:eu-repo/semantics/altIdentifier/issn/0364-3190info:eu-repo/semantics/altIdentifier/doi/10.1007/s11064-018-2628-2info:eu-repo/semantics/altIdentifier/pmid/30196348info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:36Zoai:sedici.unlp.edu.ar:10915/131399Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:37.027SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
title Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
spellingShingle Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
Sisti, María Susana
Ciencias Veterinarias
Spinal cord injury
Local anesthetic
Drug delivery
Toxic effect
title_short Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
title_full Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
title_fullStr Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
title_full_unstemmed Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
title_sort Effects of an intraparenchymal injection of lidocaine in the rat cervical spinal cord
dc.creator.none.fl_str_mv Sisti, María Susana
Zanuzzi, Carolina Natalia
Nishida, Fabián
Cantet, Rodolfo Juan Carlos
Portiansky, Enrique Leo
author Sisti, María Susana
author_facet Sisti, María Susana
Zanuzzi, Carolina Natalia
Nishida, Fabián
Cantet, Rodolfo Juan Carlos
Portiansky, Enrique Leo
author_role author
author2 Zanuzzi, Carolina Natalia
Nishida, Fabián
Cantet, Rodolfo Juan Carlos
Portiansky, Enrique Leo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Spinal cord injury
Local anesthetic
Drug delivery
Toxic effect
topic Ciencias Veterinarias
Spinal cord injury
Local anesthetic
Drug delivery
Toxic effect
dc.description.none.fl_txt_mv Lidocaine effects in the spinal cord have been extensively investigated over the years. Although the intrathecal route is usually used to treat insults occurring in the spinal cord, the local delivery drug via intraparenchymal infusions has gained increasing favor for the treatment of some neurodegenerative disorders. The aim of the present study was to evaluate the behavioral and tissue effects of the intraparenchymal injection of different concentrations of lidocaine into the rat cervical spinal cord. Young male Sprague-Dawley rats were intraparenchymally injected with 0.5%, 1% or 2% lidocaine at the C5 segment of the spinal cord. Other rats were injected with saline solution (sham group). Hot plate test was determined at 0, 1, 2, 3, 7 and 14 post-injection (pi) days. Rats of each experimental group were euthanized either at 1, 2, 3, 7 or 14 pi days. Intact animals were used as controls. Sections of the C5 segment were used for histological, immunohistochemical or immunofluorescence analysis. Injection of 0.5% lidocaine did not affect neuronal counting, did not evoke an inflammatory reaction, nor induce astrocyte activation. Therefore, a concentration of 0.5% lidocaine is suggested to promote anti-inflammatory effects after injury.
Laboratorio de Análisis de Imágenes
description Lidocaine effects in the spinal cord have been extensively investigated over the years. Although the intrathecal route is usually used to treat insults occurring in the spinal cord, the local delivery drug via intraparenchymal infusions has gained increasing favor for the treatment of some neurodegenerative disorders. The aim of the present study was to evaluate the behavioral and tissue effects of the intraparenchymal injection of different concentrations of lidocaine into the rat cervical spinal cord. Young male Sprague-Dawley rats were intraparenchymally injected with 0.5%, 1% or 2% lidocaine at the C5 segment of the spinal cord. Other rats were injected with saline solution (sham group). Hot plate test was determined at 0, 1, 2, 3, 7 and 14 post-injection (pi) days. Rats of each experimental group were euthanized either at 1, 2, 3, 7 or 14 pi days. Intact animals were used as controls. Sections of the C5 segment were used for histological, immunohistochemical or immunofluorescence analysis. Injection of 0.5% lidocaine did not affect neuronal counting, did not evoke an inflammatory reaction, nor induce astrocyte activation. Therefore, a concentration of 0.5% lidocaine is suggested to promote anti-inflammatory effects after injury.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/131399
url http://sedici.unlp.edu.ar/handle/10915/131399
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1573-6903
info:eu-repo/semantics/altIdentifier/issn/0364-3190
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11064-018-2628-2
info:eu-repo/semantics/altIdentifier/pmid/30196348
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
2072-2080
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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