Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants
- Autores
- Fait, María Elisa; Hermet, Melisa; Comelles, Francesc; Clapés, Pere; Álvarez, Hugo Ariel; Prieto, Eduardo Daniel; Herlax, Vanesa Silvana; Morcelle del Valle, Susana Raquel; Bakás, Laura Susana
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn via atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape observed at different surfactant concentrations led to the conclusion that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by a shedding of microvesicles was responsible for the hemolysis produced by both surfactants at the lower concentrations tested. In contrast, the extraction of membrane lipids upon collisions between HRBCs and surfactant aggregates competes with and prevents microvesicle release at the higher concentrations assayed.
Facultad de Ciencias Exactas
Facultad de Ciencias Médicas
Centro de Investigación de Proteínas Vegetales
Instituto de Física de Líquidos y Sistemas Biológicos
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas
Instituto de Investigaciones Bioquímicas de La Plata - Materia
-
Biología
human red-blood-cell - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/80273
Ver los metadatos del registro completo
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Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactantsFait, María ElisaHermet, MelisaComelles, FrancescClapés, PereÁlvarez, Hugo ArielPrieto, Eduardo DanielHerlax, Vanesa SilvanaMorcelle del Valle, Susana RaquelBakás, Laura SusanaBiologíahuman red-blood-cellTwo novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn via atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape observed at different surfactant concentrations led to the conclusion that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by a shedding of microvesicles was responsible for the hemolysis produced by both surfactants at the lower concentrations tested. In contrast, the extraction of membrane lipids upon collisions between HRBCs and surfactant aggregates competes with and prevents microvesicle release at the higher concentrations assayed.Facultad de Ciencias ExactasFacultad de Ciencias MédicasCentro de Investigación de Proteínas VegetalesInstituto de Física de Líquidos y Sistemas BiológicosInstituto de Investigaciones Fisicoquímicas Teóricas y AplicadasInstituto de Investigaciones Bioquímicas de La Plata2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf37549-37558http://sedici.unlp.edu.ar/handle/10915/80273enginfo:eu-repo/semantics/altIdentifier/issn/2046-2069info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:06:39Zoai:sedici.unlp.edu.ar:10915/80273Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:06:39.753SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| title |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| spellingShingle |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants Fait, María Elisa Biología human red-blood-cell |
| title_short |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| title_full |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| title_fullStr |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| title_full_unstemmed |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| title_sort |
Microvesicle release and micellar attack as the alternative mechanisms involved in the red-bloodcell-membrane solubilization induced by argininebased surfactants |
| dc.creator.none.fl_str_mv |
Fait, María Elisa Hermet, Melisa Comelles, Francesc Clapés, Pere Álvarez, Hugo Ariel Prieto, Eduardo Daniel Herlax, Vanesa Silvana Morcelle del Valle, Susana Raquel Bakás, Laura Susana |
| author |
Fait, María Elisa |
| author_facet |
Fait, María Elisa Hermet, Melisa Comelles, Francesc Clapés, Pere Álvarez, Hugo Ariel Prieto, Eduardo Daniel Herlax, Vanesa Silvana Morcelle del Valle, Susana Raquel Bakás, Laura Susana |
| author_role |
author |
| author2 |
Hermet, Melisa Comelles, Francesc Clapés, Pere Álvarez, Hugo Ariel Prieto, Eduardo Daniel Herlax, Vanesa Silvana Morcelle del Valle, Susana Raquel Bakás, Laura Susana |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Biología human red-blood-cell |
| topic |
Biología human red-blood-cell |
| dc.description.none.fl_txt_mv |
Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn via atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape observed at different surfactant concentrations led to the conclusion that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by a shedding of microvesicles was responsible for the hemolysis produced by both surfactants at the lower concentrations tested. In contrast, the extraction of membrane lipids upon collisions between HRBCs and surfactant aggregates competes with and prevents microvesicle release at the higher concentrations assayed. Facultad de Ciencias Exactas Facultad de Ciencias Médicas Centro de Investigación de Proteínas Vegetales Instituto de Física de Líquidos y Sistemas Biológicos Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas Instituto de Investigaciones Bioquímicas de La Plata |
| description |
Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn via atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape observed at different surfactant concentrations led to the conclusion that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by a shedding of microvesicles was responsible for the hemolysis produced by both surfactants at the lower concentrations tested. In contrast, the extraction of membrane lipids upon collisions between HRBCs and surfactant aggregates competes with and prevents microvesicle release at the higher concentrations assayed. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://sedici.unlp.edu.ar/handle/10915/80273 |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/2046-2069 |
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