Genomics of high molecular weight plasmids isolated from an on-farm biopurification system

Autores
Martini, María Carla; Wibberg, Daniel; Lozano, Mauricio Javier; Torres Tejerizo, Gonzalo Arturo; Albicoro, Francisco Javier; Jaenicke, Sebastián; van Elsas, Jan Dirk; Petroni, Alejandro; Garcillan Barcia, María Pilar; De La Cruz, Fernando; Schlüter, Andreas; Pühler, Alfred; Pistorio, Mariano; Lagares, Antonio; Del Papa, María Florencia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.
Instituto de Biotecnologia y Biologia Molecular
Materia
Biología
Mobile genetic
Plasmids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/86244
Acceder
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network_name_str SEDICI (UNLP)
spelling Genomics of high molecular weight plasmids isolated from an on-farm biopurification systemMartini, María CarlaWibberg, DanielLozano, Mauricio JavierTorres Tejerizo, Gonzalo ArturoAlbicoro, Francisco JavierJaenicke, Sebastiánvan Elsas, Jan DirkPetroni, AlejandroGarcillan Barcia, María PilarDe La Cruz, FernandoSchlüter, AndreasPühler, AlfredPistorio, MarianoLagares, AntonioDel Papa, María FlorenciaBiologíaMobile geneticPlasmidsThe use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.Instituto de Biotecnologia y Biologia Molecular2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86244enginfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/doi/10.1038/srep28284info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T09:59:32Zoai:sedici.unlp.edu.ar:10915/86244Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 09:59:32.241SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
title Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
spellingShingle Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
Martini, María Carla
Biología
Mobile genetic
Plasmids
title_short Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
title_full Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
title_fullStr Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
title_full_unstemmed Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
title_sort Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
dc.creator.none.fl_str_mv Martini, María Carla
Wibberg, Daniel
Lozano, Mauricio Javier
Torres Tejerizo, Gonzalo Arturo
Albicoro, Francisco Javier
Jaenicke, Sebastián
van Elsas, Jan Dirk
Petroni, Alejandro
Garcillan Barcia, María Pilar
De La Cruz, Fernando
Schlüter, Andreas
Pühler, Alfred
Pistorio, Mariano
Lagares, Antonio
Del Papa, María Florencia
author Martini, María Carla
author_facet Martini, María Carla
Wibberg, Daniel
Lozano, Mauricio Javier
Torres Tejerizo, Gonzalo Arturo
Albicoro, Francisco Javier
Jaenicke, Sebastián
van Elsas, Jan Dirk
Petroni, Alejandro
Garcillan Barcia, María Pilar
De La Cruz, Fernando
Schlüter, Andreas
Pühler, Alfred
Pistorio, Mariano
Lagares, Antonio
Del Papa, María Florencia
author_role author
author2 Wibberg, Daniel
Lozano, Mauricio Javier
Torres Tejerizo, Gonzalo Arturo
Albicoro, Francisco Javier
Jaenicke, Sebastián
van Elsas, Jan Dirk
Petroni, Alejandro
Garcillan Barcia, María Pilar
De La Cruz, Fernando
Schlüter, Andreas
Pühler, Alfred
Pistorio, Mariano
Lagares, Antonio
Del Papa, María Florencia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Mobile genetic
Plasmids
topic Biología
Mobile genetic
Plasmids
dc.description.none.fl_txt_mv The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.
Instituto de Biotecnologia y Biologia Molecular
description The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/86244
url http://sedici.unlp.edu.ar/handle/10915/86244
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/doi/10.1038/srep28284
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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