Genomics of high molecular weight plasmids isolated from an on-farm biopurification system
- Autores
- Martini, María C; Wibberg, Daniel; Lozano, Mauricio; Torres Tejerizo, Gonzalo; Albicoro, Francisco J; Jaenicke, Sebastian; van Elsas, Jan Dirk; Petroni, Alejandro; Garcillán-Barcia, M Pilar; de la Cruz, Fernando; Schlüter, Andreas; Pühler, Alfred; Pistorio, Mariano; Lagares, Antonio; Del Papa, María F
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Martini, María C. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Wibberg, Daniel. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.
Fil: Lozano, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Torres Tejerizo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Albicoro, Francisco J. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Jaenicke, Sebastian. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.
Fil: van Elsas, Jan Dirk. University of Groningen. Groningen Institute for Evolutionary Life Sciences. Microbial Ecology; Países Bajos.
Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.
Fil: Garcillán-Barcia, M Pilar. Consejo Superior de Investigaciones Científicas. Universidad de Cantabria. Instituto de Biomedicina y Biotecnología de Cantabria; España.
Fil: Cruz, Fernando de la. Consejo Superior de Investigaciones Científicas. Universidad de Cantabria. Instituto de Biomedicina y Biotecnología de Cantabria; España.
Fil: Schlüter, Andreas. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.
Fil: Pühler, Alfred. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
Fil: Papa, María F Del. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.
The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities. - Fuente
- Scientific report 2016; 6:28284
- Materia
-
Escherichia coli
Peso Molecular
Plásmidos
Replicón - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/2044
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Genomics of high molecular weight plasmids isolated from an on-farm biopurification systemMartini, María CWibberg, DanielLozano, MauricioTorres Tejerizo, GonzaloAlbicoro, Francisco JJaenicke, Sebastianvan Elsas, Jan DirkPetroni, AlejandroGarcillán-Barcia, M Pilarde la Cruz, FernandoSchlüter, AndreasPühler, AlfredPistorio, MarianoLagares, AntonioDel Papa, María FEscherichia coliPeso MolecularPlásmidosReplicónFil: Martini, María C. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Wibberg, Daniel. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.Fil: Lozano, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Torres Tejerizo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Albicoro, Francisco J. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Jaenicke, Sebastian. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.Fil: van Elsas, Jan Dirk. University of Groningen. Groningen Institute for Evolutionary Life Sciences. Microbial Ecology; Países Bajos.Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina.Fil: Garcillán-Barcia, M Pilar. Consejo Superior de Investigaciones Científicas. Universidad de Cantabria. Instituto de Biomedicina y Biotecnología de Cantabria; España.Fil: Cruz, Fernando de la. Consejo Superior de Investigaciones Científicas. Universidad de Cantabria. Instituto de Biomedicina y Biotecnología de Cantabria; España.Fil: Schlüter, Andreas. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.Fil: Pühler, Alfred. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania.Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.Fil: Papa, María F Del. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina.The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities.Nature Research2016-06info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://sgc.anlis.gob.ar/handle/123456789/204410.1038/srep28284Scientific report 2016; 6:28284reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLIS#PLACEHOLDER_PARENT_METADATA_VALUE#datasetsScientific reportsenginfo:eu-repo/semantics/openAccess2025-09-11T10:51:38Zoai:sgc.anlis.gob.ar:123456789/2044Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-11 10:51:38.556Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
title |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
spellingShingle |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system Martini, María C Escherichia coli Peso Molecular Plásmidos Replicón |
title_short |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
title_full |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
title_fullStr |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
title_full_unstemmed |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
title_sort |
Genomics of high molecular weight plasmids isolated from an on-farm biopurification system |
dc.creator.none.fl_str_mv |
Martini, María C Wibberg, Daniel Lozano, Mauricio Torres Tejerizo, Gonzalo Albicoro, Francisco J Jaenicke, Sebastian van Elsas, Jan Dirk Petroni, Alejandro Garcillán-Barcia, M Pilar de la Cruz, Fernando Schlüter, Andreas Pühler, Alfred Pistorio, Mariano Lagares, Antonio Del Papa, María F |
author |
Martini, María C |
author_facet |
Martini, María C Wibberg, Daniel Lozano, Mauricio Torres Tejerizo, Gonzalo Albicoro, Francisco J Jaenicke, Sebastian van Elsas, Jan Dirk Petroni, Alejandro Garcillán-Barcia, M Pilar de la Cruz, Fernando Schlüter, Andreas Pühler, Alfred Pistorio, Mariano Lagares, Antonio Del Papa, María F |
author_role |
author |
author2 |
Wibberg, Daniel Lozano, Mauricio Torres Tejerizo, Gonzalo Albicoro, Francisco J Jaenicke, Sebastian van Elsas, Jan Dirk Petroni, Alejandro Garcillán-Barcia, M Pilar de la Cruz, Fernando Schlüter, Andreas Pühler, Alfred Pistorio, Mariano Lagares, Antonio Del Papa, María F |
author2_role |
author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Escherichia coli Peso Molecular Plásmidos Replicón |
topic |
Escherichia coli Peso Molecular Plásmidos Replicón |
dc.description.none.fl_txt_mv |
Fil: Martini, María C. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Wibberg, Daniel. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania. Fil: Lozano, Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Torres Tejerizo, Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Albicoro, Francisco J. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Jaenicke, Sebastian. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania. Fil: van Elsas, Jan Dirk. University of Groningen. Groningen Institute for Evolutionary Life Sciences. Microbial Ecology; Países Bajos. Fil: Petroni, Alejandro. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Servicio Antimicrobianos; Argentina. Fil: Garcillán-Barcia, M Pilar. Consejo Superior de Investigaciones Científicas. Universidad de Cantabria. Instituto de Biomedicina y Biotecnología de Cantabria; España. Fil: Cruz, Fernando de la. Consejo Superior de Investigaciones Científicas. Universidad de Cantabria. Instituto de Biomedicina y Biotecnología de Cantabria; España. Fil: Schlüter, Andreas. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania. Fil: Pühler, Alfred. Bielefeld University. Institute for Genome Research and Systems Biology. Center for Biotechnology; Alemania. Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Papa, María F Del. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. The use of biopurification systems (BPS) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. BPS environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (MGEs), which play a key role in bacterial adaptation and evolution in such environments. Here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-farm BPS. The high-throughput-sequencing of the plasmid pool yielded a total of several Mb sequence information. Assembly of the sequence data resulted in six complete replicons. Using in silico analyses we identified plasmid replication genes whose encoding proteins represent 13 different Pfam families, as well as proteins involved in plasmid conjugation, indicating a large diversity of plasmid replicons and suggesting the occurrence of horizontal gene transfer (HGT) events within the habitat analyzed. In addition, genes conferring resistance to 10 classes of antimicrobial compounds and those encoding enzymes potentially involved in pesticide and aromatic hydrocarbon degradation were found. Global analysis of the plasmid pool suggest that the analyzed BPS represents a key environment for further studies addressing the dissemination of MGEs carrying catabolic genes and pathway assembly regarding degradation capabilities. |
description |
Fil: Martini, María C. Consejo Nacional de Investigaciones Científicas y Técnicas de Argentina. Instituto de Biotecnología y Biología Molecular; Argentina. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-06 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sgc.anlis.gob.ar/handle/123456789/2044 10.1038/srep28284 |
url |
http://sgc.anlis.gob.ar/handle/123456789/2044 |
identifier_str_mv |
10.1038/srep28284 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# datasets Scientific reports |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
Scientific report 2016; 6:28284 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
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Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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ANLIS |
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ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
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biblioteca@anlis.gov.ar |
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