Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats

Autores
Salto, Ileana Paula; Torres Tejerizo, Gonzalo Arturo; Wibberg, Daniel; Pühler, Alfred; Schlüter, Andreas; Pistorio, Mariano
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bacteria belonging to the genus Acinetobacter have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among Acinetobacter strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental Acinetobacter spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all Acinetobacter Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQAci, composed exclusively of Acinetobacter relaxases. Functional analysis of proteins belonging to this group showed that they behaved differently when mobilized using helper plasmids belonging to different incompatibility groups.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Exactas
Biología
Acinetobacter
plasmids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/104526
Acceder
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network_name_str SEDICI (UNLP)
spelling Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitatsSalto, Ileana PaulaTorres Tejerizo, Gonzalo ArturoWibberg, DanielPühler, AlfredSchlüter, AndreasPistorio, MarianoCiencias ExactasBiologíaAcinetobacterplasmidsBacteria belonging to the genus <i>Acinetobacter</i> have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among <i>Acinetobacter</i> strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental <i>Acinetobacter</i> spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all <i>Acinetobacter</i> Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQ<sub>Aci</sub>, composed exclusively of <i>Acinetobacter</i> relaxases. Functional analysis of proteins belonging to this group showed that they behaved differently when mobilized using helper plasmids belonging to different incompatibility groups.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/104526enginfo:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/96690info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-26180-3info:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-26180-3info:eu-repo/semantics/altIdentifier/hdl/11336/96690info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:14:39Zoai:sedici.unlp.edu.ar:10915/104526Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:14:40.205SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
spellingShingle Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
Salto, Ileana Paula
Ciencias Exactas
Biología
Acinetobacter
plasmids
title_short Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_full Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_fullStr Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_full_unstemmed Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
title_sort Comparative genomic analysis of <i>Acinetobacter</i> spp. plasmids originating from clinical settings and environmental habitats
dc.creator.none.fl_str_mv Salto, Ileana Paula
Torres Tejerizo, Gonzalo Arturo
Wibberg, Daniel
Pühler, Alfred
Schlüter, Andreas
Pistorio, Mariano
author Salto, Ileana Paula
author_facet Salto, Ileana Paula
Torres Tejerizo, Gonzalo Arturo
Wibberg, Daniel
Pühler, Alfred
Schlüter, Andreas
Pistorio, Mariano
author_role author
author2 Torres Tejerizo, Gonzalo Arturo
Wibberg, Daniel
Pühler, Alfred
Schlüter, Andreas
Pistorio, Mariano
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
Acinetobacter
plasmids
topic Ciencias Exactas
Biología
Acinetobacter
plasmids
dc.description.none.fl_txt_mv Bacteria belonging to the genus <i>Acinetobacter</i> have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among <i>Acinetobacter</i> strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental <i>Acinetobacter</i> spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all <i>Acinetobacter</i> Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQ<sub>Aci</sub>, composed exclusively of <i>Acinetobacter</i> relaxases. Functional analysis of proteins belonging to this group showed that they behaved differently when mobilized using helper plasmids belonging to different incompatibility groups.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
description Bacteria belonging to the genus <i>Acinetobacter</i> have become of clinical importance over the last decade due to the development of a multi-resistant phenotype and their ability to survive under multiple environmental conditions. The development of these traits among <i>Acinetobacter</i> strains occurs frequently as a result of plasmid-mediated horizontal gene transfer. In this work, plasmids from nosocomial and environmental <i>Acinetobacter</i> spp. collections were separately sequenced and characterized. Assembly of the sequenced data resulted in 19 complete replicons in the nosocomial collection and 77 plasmid contigs in the environmental collection. Comparative genomic analysis showed that many of them had conserved backbones. Plasmid coding sequences corresponding to plasmid specific functions were bioinformatically and functionally analyzed. Replication initiation protein analysis revealed the predominance of the Rep_3 superfamily. The phylogenetic tree constructed from all <i>Acinetobacter</i> Rep_3 superfamily plasmids showed 16 intermingled clades originating from nosocomial and environmental habitats. Phylogenetic analysis of relaxase proteins revealed the presence of a new sub-clade named MOBQ<sub>Aci</sub>, composed exclusively of <i>Acinetobacter</i> relaxases. Functional analysis of proteins belonging to this group showed that they behaved differently when mobilized using helper plasmids belonging to different incompatibility groups.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/104526
url http://sedici.unlp.edu.ar/handle/10915/104526
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://hdl.handle.net/11336/96690
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41598-018-26180-3
info:eu-repo/semantics/altIdentifier/issn/2045-2322
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-26180-3
info:eu-repo/semantics/altIdentifier/hdl/11336/96690
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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