Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal

Autores
Martini, María Carla; Albicoro, Francisco Javier; Nour, Eman; Schlüter, Andreas; van Elsas,Jan Dirk; Springael, Dirk; Smalla, Kornelia; Pistorio, Mariano; Lagares, Antonio; del Papa, Maria Florencia
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biopurification systems (BPS) are complex soil-related and artificially-generated environmentsusually designed for the removal of toxic compounds from contaminatedwastewaters.The present study has been conducted to isolate and characterize a collection of cultivableplasmid-carrying bacterial isolates recovered from a BPS established for thedecontamination of wastewater generated in a farmyard.Out of 1400 isolates, a collection of 75 plasmid-containing bacteria was obtained, ofwhich 35 representative isolates comprising in total at least 50 plasmids were chosen forfurther characterization. Bacterial hosts were taxonomically assigned by 16S ribosomal RNAgene sequencing and phenotypically characterized according to their ability to grow in presenceof different antibiotics and heavy metals. The study demonstrated that a high proportionof the isolates was tolerant to antibiotics and/or heavy metals, highlighting the on-farmBPS enrichment in such genetic traits. Several plasmids conferring such resistances in thebacterial collection were detected to be either mobilizable or selftransmissible. Occurrenceof broad host range plasmids of the incompatibility groups IncP, IncQ, IncN and IncWwas examined with positive results only for the first group. Presence of the IS1071 insertionsequence, frequently associated with xenobiotics degradation genes, was detected inDNA obtained from 24 of these isolates, strongly suggesting the presence of yet-hiddencatabolic activities in the collection of isolates. The results showed a remarkable diversityin the plasmid mobilome of cultivable bacteria in the BPS with the presence of abundantresistance markers of different types, thus providing a suitable environment to investigatethe genetic structure of the mobile genetic pool in a model on-farm biofilter forwastewater decontamination in intensive agricultural production.
Fil: Martini, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Albicoro, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Nour, Eman. Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen; Alemania
Fil: Schlüter, Andreas. Universitat Bielefeld; Alemania
Fil: van Elsas,Jan Dirk. University of Agriculture Wageningen; Países Bajos
Fil: Springael, Dirk. Katholikie Universiteit Leuven; Bélgica
Fil: Smalla, Kornelia. Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen; Alemania
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: del Papa, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Materia
Plasmid
Biopurification System
Resistance
Mobile Genetic Element
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47983
Acceder
id CONICETDig_efb1081b662c4c57dc2bdd36111aae56
oai_identifier_str oai:ri.conicet.gov.ar:11336/47983
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removalMartini, María CarlaAlbicoro, Francisco JavierNour, EmanSchlüter, Andreasvan Elsas,Jan DirkSpringael, DirkSmalla, KorneliaPistorio, MarianoLagares, Antoniodel Papa, Maria FlorenciaPlasmidBiopurification SystemResistanceMobile Genetic Elementhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Biopurification systems (BPS) are complex soil-related and artificially-generated environmentsusually designed for the removal of toxic compounds from contaminatedwastewaters.The present study has been conducted to isolate and characterize a collection of cultivableplasmid-carrying bacterial isolates recovered from a BPS established for thedecontamination of wastewater generated in a farmyard.Out of 1400 isolates, a collection of 75 plasmid-containing bacteria was obtained, ofwhich 35 representative isolates comprising in total at least 50 plasmids were chosen forfurther characterization. Bacterial hosts were taxonomically assigned by 16S ribosomal RNAgene sequencing and phenotypically characterized according to their ability to grow in presenceof different antibiotics and heavy metals. The study demonstrated that a high proportionof the isolates was tolerant to antibiotics and/or heavy metals, highlighting the on-farmBPS enrichment in such genetic traits. Several plasmids conferring such resistances in thebacterial collection were detected to be either mobilizable or selftransmissible. Occurrenceof broad host range plasmids of the incompatibility groups IncP, IncQ, IncN and IncWwas examined with positive results only for the first group. Presence of the IS1071 insertionsequence, frequently associated with xenobiotics degradation genes, was detected inDNA obtained from 24 of these isolates, strongly suggesting the presence of yet-hiddencatabolic activities in the collection of isolates. The results showed a remarkable diversityin the plasmid mobilome of cultivable bacteria in the BPS with the presence of abundantresistance markers of different types, thus providing a suitable environment to investigatethe genetic structure of the mobile genetic pool in a model on-farm biofilter forwastewater decontamination in intensive agricultural production.Fil: Martini, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Albicoro, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Nour, Eman. Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen; AlemaniaFil: Schlüter, Andreas. Universitat Bielefeld; AlemaniaFil: van Elsas,Jan Dirk. University of Agriculture Wageningen; Países BajosFil: Springael, Dirk. Katholikie Universiteit Leuven; BélgicaFil: Smalla, Kornelia. Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen; AlemaniaFil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: del Papa, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaAcademic Press Inc Elsevier Science2015-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47983Martini, María Carla; Albicoro, Francisco Javier; Nour, Eman; Schlüter, Andreas; van Elsas,Jan Dirk; et al.; Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal; Academic Press Inc Elsevier Science; Plasmid; 80; 7-2015; 16-230147-619XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plasmid.2015.05.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147619X15000438info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:40:49Zoai:ri.conicet.gov.ar:11336/47983instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:40:50.191CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
title Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
spellingShingle Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
Martini, María Carla
Plasmid
Biopurification System
Resistance
Mobile Genetic Element
title_short Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
title_full Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
title_fullStr Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
title_full_unstemmed Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
title_sort Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal
dc.creator.none.fl_str_mv Martini, María Carla
Albicoro, Francisco Javier
Nour, Eman
Schlüter, Andreas
van Elsas,Jan Dirk
Springael, Dirk
Smalla, Kornelia
Pistorio, Mariano
Lagares, Antonio
del Papa, Maria Florencia
author Martini, María Carla
author_facet Martini, María Carla
Albicoro, Francisco Javier
Nour, Eman
Schlüter, Andreas
van Elsas,Jan Dirk
Springael, Dirk
Smalla, Kornelia
Pistorio, Mariano
Lagares, Antonio
del Papa, Maria Florencia
author_role author
author2 Albicoro, Francisco Javier
Nour, Eman
Schlüter, Andreas
van Elsas,Jan Dirk
Springael, Dirk
Smalla, Kornelia
Pistorio, Mariano
Lagares, Antonio
del Papa, Maria Florencia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plasmid
Biopurification System
Resistance
Mobile Genetic Element
topic Plasmid
Biopurification System
Resistance
Mobile Genetic Element
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Biopurification systems (BPS) are complex soil-related and artificially-generated environmentsusually designed for the removal of toxic compounds from contaminatedwastewaters.The present study has been conducted to isolate and characterize a collection of cultivableplasmid-carrying bacterial isolates recovered from a BPS established for thedecontamination of wastewater generated in a farmyard.Out of 1400 isolates, a collection of 75 plasmid-containing bacteria was obtained, ofwhich 35 representative isolates comprising in total at least 50 plasmids were chosen forfurther characterization. Bacterial hosts were taxonomically assigned by 16S ribosomal RNAgene sequencing and phenotypically characterized according to their ability to grow in presenceof different antibiotics and heavy metals. The study demonstrated that a high proportionof the isolates was tolerant to antibiotics and/or heavy metals, highlighting the on-farmBPS enrichment in such genetic traits. Several plasmids conferring such resistances in thebacterial collection were detected to be either mobilizable or selftransmissible. Occurrenceof broad host range plasmids of the incompatibility groups IncP, IncQ, IncN and IncWwas examined with positive results only for the first group. Presence of the IS1071 insertionsequence, frequently associated with xenobiotics degradation genes, was detected inDNA obtained from 24 of these isolates, strongly suggesting the presence of yet-hiddencatabolic activities in the collection of isolates. The results showed a remarkable diversityin the plasmid mobilome of cultivable bacteria in the BPS with the presence of abundantresistance markers of different types, thus providing a suitable environment to investigatethe genetic structure of the mobile genetic pool in a model on-farm biofilter forwastewater decontamination in intensive agricultural production.
Fil: Martini, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Albicoro, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Nour, Eman. Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen; Alemania
Fil: Schlüter, Andreas. Universitat Bielefeld; Alemania
Fil: van Elsas,Jan Dirk. University of Agriculture Wageningen; Países Bajos
Fil: Springael, Dirk. Katholikie Universiteit Leuven; Bélgica
Fil: Smalla, Kornelia. Julius Kühn-Institut – Bundesforschungsinstitut für Kulturpflanzen; Alemania
Fil: Pistorio, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Lagares, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: del Papa, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
description Biopurification systems (BPS) are complex soil-related and artificially-generated environmentsusually designed for the removal of toxic compounds from contaminatedwastewaters.The present study has been conducted to isolate and characterize a collection of cultivableplasmid-carrying bacterial isolates recovered from a BPS established for thedecontamination of wastewater generated in a farmyard.Out of 1400 isolates, a collection of 75 plasmid-containing bacteria was obtained, ofwhich 35 representative isolates comprising in total at least 50 plasmids were chosen forfurther characterization. Bacterial hosts were taxonomically assigned by 16S ribosomal RNAgene sequencing and phenotypically characterized according to their ability to grow in presenceof different antibiotics and heavy metals. The study demonstrated that a high proportionof the isolates was tolerant to antibiotics and/or heavy metals, highlighting the on-farmBPS enrichment in such genetic traits. Several plasmids conferring such resistances in thebacterial collection were detected to be either mobilizable or selftransmissible. Occurrenceof broad host range plasmids of the incompatibility groups IncP, IncQ, IncN and IncWwas examined with positive results only for the first group. Presence of the IS1071 insertionsequence, frequently associated with xenobiotics degradation genes, was detected inDNA obtained from 24 of these isolates, strongly suggesting the presence of yet-hiddencatabolic activities in the collection of isolates. The results showed a remarkable diversityin the plasmid mobilome of cultivable bacteria in the BPS with the presence of abundantresistance markers of different types, thus providing a suitable environment to investigatethe genetic structure of the mobile genetic pool in a model on-farm biofilter forwastewater decontamination in intensive agricultural production.
publishDate 2015
dc.date.none.fl_str_mv 2015-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47983
Martini, María Carla; Albicoro, Francisco Javier; Nour, Eman; Schlüter, Andreas; van Elsas,Jan Dirk; et al.; Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal; Academic Press Inc Elsevier Science; Plasmid; 80; 7-2015; 16-23
0147-619X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47983
identifier_str_mv Martini, María Carla; Albicoro, Francisco Javier; Nour, Eman; Schlüter, Andreas; van Elsas,Jan Dirk; et al.; Characterization of a collection of plasmid-containing bacteria isolated from an on-farm biopurification system used for pesticide removal; Academic Press Inc Elsevier Science; Plasmid; 80; 7-2015; 16-23
0147-619X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plasmid.2015.05.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0147619X15000438
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.221938

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