Antimicrobial activity of Latin American medicinal plant extracts

Autores
Haag, Griselda Octavia; Valle, María Elena del; Debenedetti, Silvia Laura; Marín, Gustavo; Brignoles, Pedro; Magariños, María del Carmen
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Resistance of pathogenic microorganisms to several drugs has increased due to the widespread use of antibiotics to treat infectious diseases. Hence, development of new antimicrobial drugs from plants is an area of active research in the search for medicinal, veterinary or agricultural industry use. For this work 19 plants species was collected, dried in the shade and oven. 20 g powder plant were macerated in cold either with dichloromethane (DCM) or methanol (MeOH) filtered, evaporated to yield determination expressed in grams per 100g of dry plant. Methods: Antibacterial activity assays of the DCM and MeOH extracts were performed by agar diffusion. Paper disks were then impregnated with 10 ul of extract (range: 340-614mg/disc for MeOH; 300-500mg/disc for DCM). Solvents of DCM or MeOH extracts were used as negative controls (NC) and Gentamicin and Cephazoline were used as positive control (PC). The microorganisms used for testing were Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853. The inoculum was incorporated to Petri plates and paper disks with extracts were added, and incubated for 24 hours at 37°C. Results: All DCM extracts tested except Gentianella parviflora, Baccharis crispa, Bauhinia candicans, Terminalia langiflora and Picrosia australis showed activity against Staphylococcus aureus. The DCM extracts of the three species of Gaillardia showed to be as well active against Escherichia coli. Only the MeOH extracts of Baccharis crispa, Gentianella achalensis, Lippia turbinata, Lippia germinata, Terminalia australis and bicolor adesmia were active against Staphylococcus aureus. Conclusion: dichloromethane extracts of Gaillardia and Protousnea poepiggii species might be important sources for the isolation of compounds with antimicrobial activity with a potential use in the pharmaceutical industry.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Biología
plants
extracts
antimicrobial activity
herbal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/38220

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network_name_str SEDICI (UNLP)
spelling Antimicrobial activity of Latin American medicinal plant extractsHaag, Griselda OctaviaValle, María Elena delDebenedetti, Silvia LauraMarín, GustavoBrignoles, PedroMagariños, María del CarmenCiencias ExactasBiologíaplantsextractsantimicrobial activityherbalResistance of pathogenic microorganisms to several drugs has increased due to the widespread use of antibiotics to treat infectious diseases. Hence, development of new antimicrobial drugs from plants is an area of active research in the search for medicinal, veterinary or agricultural industry use. For this work 19 plants species was collected, dried in the shade and oven. 20 g powder plant were macerated in cold either with dichloromethane (DCM) or methanol (MeOH) filtered, evaporated to yield determination expressed in grams per 100g of dry plant. Methods: Antibacterial activity assays of the DCM and MeOH extracts were performed by agar diffusion. Paper disks were then impregnated with 10 ul of extract (range: 340-614mg/disc for MeOH; 300-500mg/disc for DCM). Solvents of DCM or MeOH extracts were used as negative controls (NC) and Gentamicin and Cephazoline were used as positive control (PC). The microorganisms used for testing were Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853. The inoculum was incorporated to Petri plates and paper disks with extracts were added, and incubated for 24 hours at 37°C. Results: All DCM extracts tested except Gentianella parviflora, Baccharis crispa, Bauhinia candicans, Terminalia langiflora and Picrosia australis showed activity against Staphylococcus aureus. The DCM extracts of the three species of Gaillardia showed to be as well active against Escherichia coli. Only the MeOH extracts of Baccharis crispa, Gentianella achalensis, Lippia turbinata, Lippia germinata, Terminalia australis and bicolor adesmia were active against Staphylococcus aureus. Conclusion: dichloromethane extracts of Gaillardia and Protousnea poepiggii species might be important sources for the isolation of compounds with antimicrobial activity with a potential use in the pharmaceutical industry.Facultad de Ciencias Exactas2014-02-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf128-131http://sedici.unlp.edu.ar/handle/10915/38220enginfo:eu-repo/semantics/altIdentifier/issn/2277‐3290info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Creative Commons Attribution-NonCommercial-NoDerivs 2.5 Argentina (CC BY-NC-ND 2.5)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:00:02Zoai:sedici.unlp.edu.ar:10915/38220Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:00:02.441SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Antimicrobial activity of Latin American medicinal plant extracts
title Antimicrobial activity of Latin American medicinal plant extracts
spellingShingle Antimicrobial activity of Latin American medicinal plant extracts
Haag, Griselda Octavia
Ciencias Exactas
Biología
plants
extracts
antimicrobial activity
herbal
title_short Antimicrobial activity of Latin American medicinal plant extracts
title_full Antimicrobial activity of Latin American medicinal plant extracts
title_fullStr Antimicrobial activity of Latin American medicinal plant extracts
title_full_unstemmed Antimicrobial activity of Latin American medicinal plant extracts
title_sort Antimicrobial activity of Latin American medicinal plant extracts
dc.creator.none.fl_str_mv Haag, Griselda Octavia
Valle, María Elena del
Debenedetti, Silvia Laura
Marín, Gustavo
Brignoles, Pedro
Magariños, María del Carmen
author Haag, Griselda Octavia
author_facet Haag, Griselda Octavia
Valle, María Elena del
Debenedetti, Silvia Laura
Marín, Gustavo
Brignoles, Pedro
Magariños, María del Carmen
author_role author
author2 Valle, María Elena del
Debenedetti, Silvia Laura
Marín, Gustavo
Brignoles, Pedro
Magariños, María del Carmen
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
plants
extracts
antimicrobial activity
herbal
topic Ciencias Exactas
Biología
plants
extracts
antimicrobial activity
herbal
dc.description.none.fl_txt_mv Resistance of pathogenic microorganisms to several drugs has increased due to the widespread use of antibiotics to treat infectious diseases. Hence, development of new antimicrobial drugs from plants is an area of active research in the search for medicinal, veterinary or agricultural industry use. For this work 19 plants species was collected, dried in the shade and oven. 20 g powder plant were macerated in cold either with dichloromethane (DCM) or methanol (MeOH) filtered, evaporated to yield determination expressed in grams per 100g of dry plant. Methods: Antibacterial activity assays of the DCM and MeOH extracts were performed by agar diffusion. Paper disks were then impregnated with 10 ul of extract (range: 340-614mg/disc for MeOH; 300-500mg/disc for DCM). Solvents of DCM or MeOH extracts were used as negative controls (NC) and Gentamicin and Cephazoline were used as positive control (PC). The microorganisms used for testing were Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853. The inoculum was incorporated to Petri plates and paper disks with extracts were added, and incubated for 24 hours at 37°C. Results: All DCM extracts tested except Gentianella parviflora, Baccharis crispa, Bauhinia candicans, Terminalia langiflora and Picrosia australis showed activity against Staphylococcus aureus. The DCM extracts of the three species of Gaillardia showed to be as well active against Escherichia coli. Only the MeOH extracts of Baccharis crispa, Gentianella achalensis, Lippia turbinata, Lippia germinata, Terminalia australis and bicolor adesmia were active against Staphylococcus aureus. Conclusion: dichloromethane extracts of Gaillardia and Protousnea poepiggii species might be important sources for the isolation of compounds with antimicrobial activity with a potential use in the pharmaceutical industry.
Facultad de Ciencias Exactas
description Resistance of pathogenic microorganisms to several drugs has increased due to the widespread use of antibiotics to treat infectious diseases. Hence, development of new antimicrobial drugs from plants is an area of active research in the search for medicinal, veterinary or agricultural industry use. For this work 19 plants species was collected, dried in the shade and oven. 20 g powder plant were macerated in cold either with dichloromethane (DCM) or methanol (MeOH) filtered, evaporated to yield determination expressed in grams per 100g of dry plant. Methods: Antibacterial activity assays of the DCM and MeOH extracts were performed by agar diffusion. Paper disks were then impregnated with 10 ul of extract (range: 340-614mg/disc for MeOH; 300-500mg/disc for DCM). Solvents of DCM or MeOH extracts were used as negative controls (NC) and Gentamicin and Cephazoline were used as positive control (PC). The microorganisms used for testing were Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, and Pseudomonas aeruginosa ATCC 27853. The inoculum was incorporated to Petri plates and paper disks with extracts were added, and incubated for 24 hours at 37°C. Results: All DCM extracts tested except Gentianella parviflora, Baccharis crispa, Bauhinia candicans, Terminalia langiflora and Picrosia australis showed activity against Staphylococcus aureus. The DCM extracts of the three species of Gaillardia showed to be as well active against Escherichia coli. Only the MeOH extracts of Baccharis crispa, Gentianella achalensis, Lippia turbinata, Lippia germinata, Terminalia australis and bicolor adesmia were active against Staphylococcus aureus. Conclusion: dichloromethane extracts of Gaillardia and Protousnea poepiggii species might be important sources for the isolation of compounds with antimicrobial activity with a potential use in the pharmaceutical industry.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-26
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/38220
url http://sedici.unlp.edu.ar/handle/10915/38220
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2277‐3290
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Creative Commons Attribution-NonCommercial-NoDerivs 2.5 Argentina (CC BY-NC-ND 2.5)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Creative Commons Attribution-NonCommercial-NoDerivs 2.5 Argentina (CC BY-NC-ND 2.5)
dc.format.none.fl_str_mv application/pdf
128-131
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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