Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)

Autores
Núñez, María; Torres, Carola Analía; Aguado, María Inés; Bela, Alberto; Dudik, Nestor Hugo; Bregni, Carlos
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Total polyphenols content was evaluated in ethanolic and aqueous extracts of leaves and inflorescences of Lippia alba (Mill.), from plants cultivated in the Chaco province, northeastern of Argentina. The extracts were obtained by percolation, infusion and decoction. Total phenols content was determined by Folin Ciocalteu reagent, flavonoids content was determined in Al-complex form and the free radical scavenging activity was determined by DPPH radical. The antimicrobial screening was carried out by bioautography and in the active extracts the minimal inhibitory concentration and minimal bactericidal concentration was determined by the disc diffusion method. Results in terms of phenols content, flavonoids content and DPPH radical scavenging were higher in ethanolic extracts than in the aqueous ones. There were differences statistically significant in phenols and flavonoids contents of the extracts obtained with extraction solvents of different polarity. The aqueous extracts obtained by infusion presented smaller phenols content, higher flavonoids content and better free radical scavenging activity in comparison with the extracts obtained by decoction. Only ethanolic extracts presented antimicrobial activity and they were more active against Gram-positive bacteria.
Fil: Núñez, María. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Torres, Carola Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas. Laboratorio de Microbiología de Alimentos; Argentina
Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Bela, Alberto. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Dudik, Nestor Hugo. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Bregni, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
Materia
Antibacterial activity
Antioxidant activity
Plants extracts
Total phenols
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274314
Acceder
id CONICETDig_db206b2273607f47a17969ca853a5376
oai_identifier_str oai:ri.conicet.gov.ar:11336/274314
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)Núñez, MaríaTorres, Carola AnalíaAguado, María InésBela, AlbertoDudik, Nestor HugoBregni, CarlosAntibacterial activityAntioxidant activityPlants extractsTotal phenolshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Total polyphenols content was evaluated in ethanolic and aqueous extracts of leaves and inflorescences of Lippia alba (Mill.), from plants cultivated in the Chaco province, northeastern of Argentina. The extracts were obtained by percolation, infusion and decoction. Total phenols content was determined by Folin Ciocalteu reagent, flavonoids content was determined in Al-complex form and the free radical scavenging activity was determined by DPPH radical. The antimicrobial screening was carried out by bioautography and in the active extracts the minimal inhibitory concentration and minimal bactericidal concentration was determined by the disc diffusion method. Results in terms of phenols content, flavonoids content and DPPH radical scavenging were higher in ethanolic extracts than in the aqueous ones. There were differences statistically significant in phenols and flavonoids contents of the extracts obtained with extraction solvents of different polarity. The aqueous extracts obtained by infusion presented smaller phenols content, higher flavonoids content and better free radical scavenging activity in comparison with the extracts obtained by decoction. Only ethanolic extracts presented antimicrobial activity and they were more active against Gram-positive bacteria.Fil: Núñez, María. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Torres, Carola Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas. Laboratorio de Microbiología de Alimentos; ArgentinaFil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Bela, Alberto. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Dudik, Nestor Hugo. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; ArgentinaFil: Bregni, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaOpen Access Science Research Publisher2012-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274314Núñez, María; Torres, Carola Analía; Aguado, María Inés; Bela, Alberto; Dudik, Nestor Hugo; et al.; Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.); Open Access Science Research Publisher; International Journal of Medicinal and Aromatic Plants; 2; 3; 9-2012; 361-3682249-4340CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.cabidigitallibrary.org/doi/pdf/10.5555/20123332296info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T09:46:01Zoai:ri.conicet.gov.ar:11336/274314instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 09:46:01.675CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
title Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
spellingShingle Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
Núñez, María
Antibacterial activity
Antioxidant activity
Plants extracts
Total phenols
title_short Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
title_full Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
title_fullStr Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
title_full_unstemmed Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
title_sort Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.)
dc.creator.none.fl_str_mv Núñez, María
Torres, Carola Analía
Aguado, María Inés
Bela, Alberto
Dudik, Nestor Hugo
Bregni, Carlos
author Núñez, María
author_facet Núñez, María
Torres, Carola Analía
Aguado, María Inés
Bela, Alberto
Dudik, Nestor Hugo
Bregni, Carlos
author_role author
author2 Torres, Carola Analía
Aguado, María Inés
Bela, Alberto
Dudik, Nestor Hugo
Bregni, Carlos
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Antibacterial activity
Antioxidant activity
Plants extracts
Total phenols
topic Antibacterial activity
Antioxidant activity
Plants extracts
Total phenols
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Total polyphenols content was evaluated in ethanolic and aqueous extracts of leaves and inflorescences of Lippia alba (Mill.), from plants cultivated in the Chaco province, northeastern of Argentina. The extracts were obtained by percolation, infusion and decoction. Total phenols content was determined by Folin Ciocalteu reagent, flavonoids content was determined in Al-complex form and the free radical scavenging activity was determined by DPPH radical. The antimicrobial screening was carried out by bioautography and in the active extracts the minimal inhibitory concentration and minimal bactericidal concentration was determined by the disc diffusion method. Results in terms of phenols content, flavonoids content and DPPH radical scavenging were higher in ethanolic extracts than in the aqueous ones. There were differences statistically significant in phenols and flavonoids contents of the extracts obtained with extraction solvents of different polarity. The aqueous extracts obtained by infusion presented smaller phenols content, higher flavonoids content and better free radical scavenging activity in comparison with the extracts obtained by decoction. Only ethanolic extracts presented antimicrobial activity and they were more active against Gram-positive bacteria.
Fil: Núñez, María. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Torres, Carola Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Chaco Austral. Departamento de Ciencias Basicas y Aplicadas. Laboratorio de Microbiología de Alimentos; Argentina
Fil: Aguado, María Inés. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Bela, Alberto. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Dudik, Nestor Hugo. Universidad Nacional del Chaco Austral. Departamento de Ciencias Básicas y Aplicadas; Argentina
Fil: Bregni, Carlos. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina
description Total polyphenols content was evaluated in ethanolic and aqueous extracts of leaves and inflorescences of Lippia alba (Mill.), from plants cultivated in the Chaco province, northeastern of Argentina. The extracts were obtained by percolation, infusion and decoction. Total phenols content was determined by Folin Ciocalteu reagent, flavonoids content was determined in Al-complex form and the free radical scavenging activity was determined by DPPH radical. The antimicrobial screening was carried out by bioautography and in the active extracts the minimal inhibitory concentration and minimal bactericidal concentration was determined by the disc diffusion method. Results in terms of phenols content, flavonoids content and DPPH radical scavenging were higher in ethanolic extracts than in the aqueous ones. There were differences statistically significant in phenols and flavonoids contents of the extracts obtained with extraction solvents of different polarity. The aqueous extracts obtained by infusion presented smaller phenols content, higher flavonoids content and better free radical scavenging activity in comparison with the extracts obtained by decoction. Only ethanolic extracts presented antimicrobial activity and they were more active against Gram-positive bacteria.
publishDate 2012
dc.date.none.fl_str_mv 2012-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274314
Núñez, María; Torres, Carola Analía; Aguado, María Inés; Bela, Alberto; Dudik, Nestor Hugo; et al.; Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.); Open Access Science Research Publisher; International Journal of Medicinal and Aromatic Plants; 2; 3; 9-2012; 361-368
2249-4340
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274314
identifier_str_mv Núñez, María; Torres, Carola Analía; Aguado, María Inés; Bela, Alberto; Dudik, Nestor Hugo; et al.; Polyphenols and antimicrobial activity in extracts of Lippia alba (Mill.); Open Access Science Research Publisher; International Journal of Medicinal and Aromatic Plants; 2; 3; 9-2012; 361-368
2249-4340
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.cabidigitallibrary.org/doi/pdf/10.5555/20123332296
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Open Access Science Research Publisher
publisher.none.fl_str_mv Open Access Science Research Publisher
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847977101030850560
score 13.084122

Publicaciones similares